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Here, we present a mechanism by which Salmonella can rapidly respond to the external environment at the translational level; namely, the dynamic acetylation changes at the K247 site of ribosomal protein S1 could modulate the different mRNAs translation to adapt to distinct infection stages. We uncovered that S1K247 preferentially recruits mRNAs associated with flagellum assembly, sulfur metabolism, and SPI-1 T3SS. Conversely, S1K247Ac catalyzed by Pat favors the mRNAs linked to arginine biosynthesis, contributing to the activation of ArgR regulating SPI-2 virulence factors and enabling survival and replication within macrophages. Notably, a K247 acetyl-mimetic mutant strain exhibited increased virulence both ex vivo and in vivo. This mechanism not only aids in further understanding how the pathogen survives in complex environment but also facilitates in identifying new targets and pathways to eliminating pathogenic bacteria.<\/jats:p>","DOI":"10.1093\/nar\/gkaf252","type":"journal-article","created":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T16:05:12Z","timestamp":1742486712000},"source":"Crossref","is-referenced-by-count":4,"title":["Reversible acetylation of ribosomal protein S1 serves as a smart switch for <i>Salmonella<\/i> to rapidly adapt to host stress"],"prefix":"10.1093","volume":"53","author":[{"given":"Yi-Lin","family":"Shen","sequence":"first","affiliation":[{"name":"Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai\u00a0 200237 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9368-4293","authenticated-orcid":false,"given":"Tian-Xian","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai\u00a0 200237 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Xu","sequence":"additional","affiliation":[{"name":"Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai\u00a0 200237 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5555-5359","authenticated-orcid":false,"given":"Bang-Ce","family":"Ye","sequence":"additional","affiliation":[{"name":"Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai\u00a0 200237 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9241-1293","authenticated-orcid":false,"given":"Ying","family":"Zhou","sequence":"additional","affiliation":[{"name":"Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai\u00a0 200237 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,4,1]]},"reference":[{"key":"2025041601015776500_B1","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1111\/j.1365-2958.2011.07612.x","article-title":"How does the oxidative burst of macrophages kill bacteria? 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