{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T22:47:46Z","timestamp":1792104466133,"version":"4.4.0"},"reference-count":74,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T00:00:00Z","timestamp":1748390400000},"content-version":"vor","delay-in-days":6,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006095","name":"Helse S\u00f8r-\u00d8st RHF","doi-asserted-by":"publisher","award":["2020043"],"award-info":[{"award-number":["2020043"]}],"id":[{"id":"10.13039\/501100006095","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006095","name":"Helse S\u00f8r-\u00d8st RHF","doi-asserted-by":"publisher","award":["2019022"],"award-info":[{"award-number":["2019022"]}],"id":[{"id":"10.13039\/501100006095","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Pasteurlegatet and Professor Th. Thj\u00f8ttas legat"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Fluoroquinolones induce double-strand breaks in bacterial DNA, triggering the SOS response, a major DNA damage response that ensures the expression of repair proteins but also promotes the emergence and spread of antibiotic resistance. Fluoroquinolone resistance, particularly in Escherichia coli, is a growing global health concern. Understanding bacterial responses to these antibiotics is critical for developing preventive strategies and novel treatments to combat resistance development. This study investigates DNA morphology in E. coli following exposure to ciprofloxacin (CIP), a fluoroquinolone antibiotic. We show that CIP induces a stepwise DNA reorganization, culminating in a highly dense nucleoid structure at midcell\u2014a process we term DNA supercompaction. This phenomenon occurred also with other genotoxic agents. Live-cell imaging revealed that RecN, a structural maintenance of chromosomes (SMC)-like protein, is required for DNA supercompaction, and that RecN\u2019s dynamics and activity in this response depend on RecA. Additionally, RecN and RecA frequently colocalized at nucleoid-associated positions. We suggest that RecN and RecA play active roles in DNA supercompaction following severe DNA damage, that their interplay is part of a prompt universal survival response to DNA double-strand breaks in E. coli, and that the extent of the compaction response depends on the DNA damage severity.<\/jats:p>","DOI":"10.1093\/nar\/gkaf437","type":"journal-article","created":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T08:07:15Z","timestamp":1747123635000},"source":"Crossref","is-referenced-by-count":15,"title":["RecN and RecA orchestrate an ordered DNA supercompaction response following ciprofloxacin-induced DNA damage in\n                    <i>Escherichia coli<\/i>"],"prefix":"10.1093","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7475-4636","authenticated-orcid":false,"given":"Krister","family":"Vikedal","sequence":"first","affiliation":[{"name":"University of Oslo and Oslo University Hospital, Rikshospitalet Department of Microbiology, , 0373 Oslo ,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1562-6343","authenticated-orcid":false,"given":"Synn\u00f8ve Brandt","family":"R\u00e6der","sequence":"additional","affiliation":[{"name":"University of Oslo and Oslo University Hospital, Rikshospitalet Department of Microbiology, , 0373 Oslo ,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida Mathilde\u00a0Marstein","family":"Riisn\u00e6s","sequence":"additional","affiliation":[{"name":"University of Oslo and Oslo University Hospital, Rikshospitalet Department of Microbiology, , 0373 Oslo ,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8759-1170","authenticated-orcid":false,"given":"Magnar","family":"Bj\u00f8r\u00e5s","sequence":"additional","affiliation":[{"name":"University of Oslo and Oslo University Hospital, Rikshospitalet Department of Microbiology, , 0373 Oslo ,","place":["Norway"]},{"name":"Norwegian University of Science and Technology Department of Clinical and Molecular Medicine, , 7030 Trondheim ,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9452-3424","authenticated-orcid":false,"given":"James Alexander","family":"Booth","sequence":"additional","affiliation":[{"name":"University of Oslo and Oslo University Hospital, Rikshospitalet Department of Microbiology, , 0373 Oslo ,","place":["Norway"]},{"name":"Norwegian University of Science and Technology Department of Clinical and Molecular Medicine, , 7030 Trondheim ,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Skarstad","sequence":"additional","affiliation":[{"name":"University of Oslo and Oslo University Hospital, Rikshospitalet Department of Microbiology, , 0373 Oslo 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