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The requirement of the RNA chaperone Hfq for the virulence of B. pertussis suggests that Hfq-dependent small regulatory RNAs (sRNAs) are involved in the virulence of this pathogen. To identify their potential mRNA targets, we applied a method combining experimental and computational approaches called RIL-seq. The majority of putative mRNA targets, including several virulence factors, interact with two sRNAs, CT_433 and CT_521, suggesting that these sRNAs may represent central riboregulatory nodes of B. pertussis. Furthermore, our data suggest that CT_532 sRNA can base pair with the 5\u2032UTR region of ompA mRNA encoding outer membrane protein BP0943 (OmpA) and that CT_532, RNase III and Hfq are involved in the control of ompA expression. The CT_532 sRNA shares 60% identity with the E. coli sRNA MicA and its expression is also modulated by Hfq and stress conditions such as heat and cold shocks. Overall, these results suggest that CT_532 represents a MicA homolog. Importantly, the mutant lacking the first 22 nucleotides of CT_532 exhibits reduced cytotoxicity towards human macrophages and impaired biofilm production but increased resistance to complement compared to the wild type strain.<\/jats:p>","DOI":"10.1093\/nar\/gkaf614","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T07:45:15Z","timestamp":1749800715000},"source":"Crossref","is-referenced-by-count":3,"title":["Global analysis of the Hfq-mediated RNA interactome discovers a MicA homolog that affects the cytotoxicity, biofilm formation, and resistance to complement of\u00a0\n                    <i>Bordetella pertussis<\/i>"],"prefix":"10.1093","volume":"53","author":[{"given":"Dilip","family":"Kumar","sequence":"first","affiliation":[{"name":"Laboratory of post-transcriptional control of gene expression, Institute of Microbiology of the Czech Academy of Sciences , Prague 14200 ,","place":["Czech 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