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However, the methods that are traditionally used to detect these repair products in human cells have several limitations, including the presence of other contaminating large DNAs and labeling efficiency. We therefore developed a novel microplate-based competitive immunoassay platform to precisely measure sedDNAs containing pyrimidine (6\u20134) pyrimidone photoproducts [(6\u20134)PPs] and cyclobutane pyrimidine dimer (CPD) following UV exposure. The immunoassay employs polyethylene glycol to separate sedDNAs from larger contaminating DNA molecules along with immobilized UV-irradiated oligonucleotides and damage-specific antibodies. Our results demonstrate that this method is highly sensitive and specific, enabling the detection of NER-dependent sedDNAs generated by minimal UV doses and within a short time frame after UV exposure. Moreover, quantification of (6\u20134)PP- and CPD-containing sedDNAs in this assay allowed the detection of up to 22-fold more (6\u20134)PP-sedDNAs and up to 7-fold more CPD-sedDNAs than by conventional 3\u2032-end labeling. Thus, this novel immunoassay shows remarkable sensitivity and accuracy for quantifying sedDNAs in UV-irradiated cells and provides a convenient platform for advancing our understanding of the molecular mechanisms of the NER system.<\/jats:p>","DOI":"10.1093\/nar\/gkag180","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T12:55:13Z","timestamp":1771505713000},"source":"Crossref","is-referenced-by-count":0,"title":["Development of a competitive immunoassay platform for quantifying the oligonucleotide products of nucleotide excision repair in UV-irradiated cells"],"prefix":"10.1093","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6855-653X","authenticated-orcid":false,"given":"Geun Hoe","family":"Kim","sequence":"first","affiliation":[{"name":"Division of Biomedical Metrology, Korea Research Institute of Standards and Science , Daejeon 305-340 ,","place":["Republic of Korea"]},{"name":"Department of Bio-Analytical Science, University of Science & Technology , Daejeon 305-340 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7133-5401","authenticated-orcid":false,"given":"Ha-Jeong","family":"Kwon","sequence":"additional","affiliation":[{"name":"Division of Chemical and Material Metrology, Korea Research Institute of Standards and Science , Daejeon 305-340 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2094-4268","authenticated-orcid":false,"given":"Youngmin","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Biomedical Metrology, Korea Research Institute of Standards and Science , Daejeon 305-340 ,","place":["Republic of Korea"]},{"name":"Department of Bio-Analytical Science, University of Science & Technology , Daejeon 305-340 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8203-0745","authenticated-orcid":false,"given":"Michael G","family":"Kemp","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Wright State University Boonshoft School of Medicine , Dayton, OH 45435 ,","place":["United States"]},{"name":"Research Service, Dayton Veterans Administration Medical Center , Dayton, OH 45428 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2271-949X","authenticated-orcid":false,"given":"Jun-Hyuk","family":"Choi","sequence":"additional","affiliation":[{"name":"Division of Biomedical Metrology, Korea Research Institute of Standards and Science , Daejeon 305-340 ,","place":["Republic of Korea"]},{"name":"Department of Bio-Analytical Science, University of Science & Technology , Daejeon 305-340 ,","place":["Republic of 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