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Its activity is determined by the combined effects of adaptation and interference. However, the dynamic regulation of these two processes remains not fully understood. In this study, we identify the LysR-type transcriptional regulator LrhA, which is differentially expressed in various Escherichia coli strains, as a novel CRISPR-Cas activator that plays a critical role in modulating host defense levels. In a representative strain expressing a high level of LrhA, the regulator enhances CRISPR-Cas-mediated adaptive immunity against bacteriophage infection by promoting cas gene transcription through direct interaction with the promoter of the cas operon. Moderate activation of cas genes by weakly expressed LrhA in another representative strain efficiently accelerates the clearance of horizontally transferred CRISPR-targeted plasmids by enhancing spacer acquisition via interference-driven adaptation. This divergence, likely a result of genome evolution, suggests that adaptive immunity is optimized with intermediate transcription levels of cas genes by triggering positive feedback between adaptation and interference. Collectively, our findings highlight the crucial role of LrhA in fine-tuning host defense responses.<\/jats:p>","DOI":"10.1093\/nar\/gkag204","type":"journal-article","created":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T12:44:37Z","timestamp":1772023477000},"source":"Crossref","is-referenced-by-count":2,"title":["LysR-type regulator LrhA promotes CRISPR-Cas immunity in\n                    <i>Escherichia coli<\/i>"],"prefix":"10.1093","volume":"54","author":[{"given":"Mengdie","family":"Fang","sequence":"first","affiliation":[{"name":"College of Biotechnology and Bioengineering, Zhejiang University of Technology , Hangzhou, Zhejiang 310014 ,","place":["China"]},{"name":"School of Laboratory Medicine and Bioengineering, Zhejiang Provincial People\u2019s Hospital (Affiliated People\u2019s Hospital), Hangzhou Medical College , Hangzhou, Zhejiang 310013 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