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Here, we uncover an RNA-based mechanism that controls translation initiation in the enterobacterial timPR system. Unlike most T1TAs, which typically rely on ribonucleolytic messenger RNA (mRNA) processing to relieve ribosome binding site sequestration, the primary timP toxin mRNA is activated through a purely structural RNA switch. Using a FASTBAC-Seq loss-of-function screen with biochemical and phenotypic assays, we here identify key RNA interactions that govern this switch. Translation initiation at timP requires formation of (i) a pseudoknot in the 5\u2032 untranslated region, and (ii) a long-range interaction that destabilizes the ribosome-binding-site-sequestering stem-loop, rendering the Shine\u2013Dalgarno sequence accessible for pre-initiation complex formation. Conversely, an alternative interaction locks the mRNA in an inactive state. Our findings reveal a structural RNA switch that controls toxin expression without the need for enzymatic processing and demonstrate an alternative mechanism for translation initiation in bacteria.<\/jats:p>","DOI":"10.1093\/nar\/gkag240","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T09:28:17Z","timestamp":1773912497000},"source":"Crossref","is-referenced-by-count":0,"title":["An RNA structural switch controlling bacterial toxin translation"],"prefix":"10.1093","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9424-0314","authenticated-orcid":false,"given":"Athina","family":"Eleftheraki","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Biology, Biomedical Center, Uppsala University , Uppsala S-75124 ,","place":["Sweden"]},{"name":"Uppsala Antibiotic Center, Uppsala University , Uppsala S-75123 ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2660-262X","authenticated-orcid":false,"given":"Andr\u00e9s","family":"Escalera-Maurer","sequence":"additional","affiliation":[{"name":"Univ. Bordeaux, CNRS, INSERM, ARNA, UMR 5320, U1212 , Bordeaux F-33000 ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4318-5643","authenticated-orcid":false,"given":"Elsa D M","family":"Hien","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Biomedical Center, Uppsala University , Uppsala S-75124 ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3812-4919","authenticated-orcid":false,"given":"Alice","family":"Virciglio","sequence":"additional","affiliation":[{"name":"Univ. Bordeaux, CNRS, INSERM, ARNA, UMR 5320, U1212 , Bordeaux F-33000 ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6065-8965","authenticated-orcid":false,"given":"Ma\u00e9va","family":"Conangle","sequence":"additional","affiliation":[{"name":"Univ. Bordeaux, CNRS, INSERM, ARNA, UMR 5320, U1212 , Bordeaux F-33000 ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7379-8729","authenticated-orcid":false,"given":"Nicolas J","family":"Tourasse","sequence":"additional","affiliation":[{"name":"Univ. Bordeaux, CNRS, INSERM, ARNA, UMR 5320, U1212 , Bordeaux F-33000 ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2211-2153","authenticated-orcid":false,"given":"Ana\u00efs","family":"Le\u00a0Rhun","sequence":"additional","affiliation":[{"name":"Univ. 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