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In mammals, these modifications are key to a transcript surveillance system that regulates the interferon response, but the broader functions of cOMe remain poorly understood. To understand the role of cOMe in Caenorhabditis elegans, we functionally characterized the methyltransferases (CMTR-1 and CMTR-2) responsible for installing these modifications. These enzymes have distinct expression patterns, protein interaction partners, and loss-of-function phenotypes. Loss of CMTR-1 causes dramatic reductions in cOMe, impaired growth, and sterility. In contrast, animals lacking CMTR-2 are superficially wild-type, though CMTR-2 loss enhances the severity of the cmtr-1 mutant phenotype. Depletion of CMTR-1 causes downregulation of transcripts associated with germline sex determination and upregulation of those involved in the intracellular pathogen response (IPR). We show that the absence of the decapping exonuclease, EOL-1, an IPR component, completely suppresses the sterility and growth defects caused by CMTR-1 loss, and reverses the associated steady-state transcript changes. Our work shows the physiological relevance of cOMe in protecting transcripts from decapping exonucleases, raising the possibility that cOMe plays a role in RNA-mediated immune surveillance beyond the vertebrates.<\/jats:p>","DOI":"10.1093\/nar\/gkag355","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T11:58:51Z","timestamp":1775649531000},"source":"Crossref","is-referenced-by-count":4,"title":["Cap-adjacent 2\u2032-\n                    <i>O<\/i>\n                    -ribose methylation of RNA in\n                    <i>C. elegans<\/i>\n                    is required for postembryonic growth and germline development in the presence of the decapping exonuclease EOL-1"],"prefix":"10.1093","volume":"54","author":[{"given":"Eileen","family":"Clemens","sequence":"first","affiliation":[{"name":"School of Medicine, Medical Sciences and Nutrition, Institute of Medical Sciences, University of Aberdeen , Aberdeen AB25 2ZD ,","place":["United 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