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As a result, residues whose native interaction partners lie downstream in sequence cannot immediately form their native contacts and remain transiently unsatisfied until those partners are synthesized. These unsatisfied residues are vulnerable to non-native interactions and often require the engagement of co-translational chaperones. We previously developed the Native Fold Delay (NFD) metric to quantify the time lag between the synthesis of a residue and the point at which it can form all its native contacts. Here, we present the FoldDelay web server, a freely accessible platform that extends the NFD concept into a more comprehensive framework for analyzing native residue\u2013residue contact formation during translation. Starting from user-submitted AlphaFold or PDB structures, the site identifies all N- to C-terminal residue\u2013residue contacts, estimates their earliest possible formation times, and integrates domain annotations to distinguish between intra- and inter-domain contacts. The server provides a suite of linked interactive visualizations that allows users to explore native contact formation dynamics and detect transiently unsatisfied regions. The FoldDelay web server is freely accessible at https:\/\/folddelay.switchlab.org.<\/jats:p>","DOI":"10.1093\/nar\/gkag402","type":"journal-article","created":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T11:16:26Z","timestamp":1776510986000},"page":"W266-W271","source":"Crossref","is-referenced-by-count":0,"title":["FoldDelay web server: an online tool to quantify translation-driven delays in protein native contact formation"],"prefix":"10.1093","volume":"54","author":[{"given":"Ramon","family":"Duran-Roma\u00f1a","sequence":"first","affiliation":[{"name":"Switch Laboratory, VIB-KU Leuven Center for Neuroscience , Herestraat 49, 3000Leuven, \u00a0","place":["Belgium"]},{"name":"Department of Cellular and Molecular Medicine, KU Leuven , Herestraat 49, 3000 Leuven ,","place":["Belgium"]},{"name":"VIB Center for AI & Computational Biology , Herestraat 49,3000Leuven 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