{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T08:57:42Z","timestamp":1781254662094,"version":"3.54.1"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"vor","delay-in-days":4,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM152032"],"award-info":[{"award-number":["R35GM152032"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NIH COBREs"},{"name":"Holland Computing Center of the University of Nebraska"},{"name":"Office of Research and Economic Development"},{"name":"Nebraska Research Initiative"},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM152032"],"award-info":[{"award-number":["R35GM152032"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Herpes simplex virus type 1 (HSV-1) causes lifelong infections in human cells and is associated with a range of diseases. HSV-1 DNA replication requires seven viral proteins, including the major DNA-binding protein ICP8, the origin-binding protein UL9, and proteins that comprise the helicase\u2013primase and DNA polymerase complexes. UL9 functions as a DNA helicase that specifically recognizes and binds to the viral origins of replication, OriS and OriL. Here we report the cryo-EM structure of the UL9\/ICP8\/DNA\/ATP\u03b3S complex at an overall resolution of 3.18 \u00c5. This structure revealed that UL9 employs an \u03b1-helix to separate the DNA strands and captures the initial step of OriS unwinding, in which the C-terminal domain of UL9 specifically binds to the major groove of a DNA double helix, while the N-terminal helicase domain engages the unwound leading and lagging strands. ICP8 interacts with the extreme C-terminal region of UL9, preventing UL9 dimerization. Simultaneously, it binds and stabilizes the leading-strand DNA adjacent to UL9. Together, these findings provide mechanistic insight into UL9-driven DNA unwinding and the cooperative action of UL9 and ICP8 at HSV-1 replication origins, establishing a structural framework for the rational interpretation of prior biochemical data and for the design of new antiviral drugs.<\/jats:p>","DOI":"10.1093\/nar\/gkag580","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T11:40:02Z","timestamp":1780141202000},"source":"Crossref","is-referenced-by-count":0,"title":["Structural insights into HSV-1 origin unwinding by the viral proteins UL9 and ICP8"],"prefix":"10.1093","volume":"54","author":[{"given":"Andrey G","family":"Baranovskiy","sequence":"first","affiliation":[{"name":"Eppley Institute for Research in Cancer and Allied Diseases, Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center , Omaha, NE 68198 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia M","family":"Morstadt","sequence":"additional","affiliation":[{"name":"Eppley Institute for Research in Cancer and Allied Diseases, Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center , Omaha, NE 68198 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo E","family":"Romero","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Nebraska-Lincoln , Lincoln, NE 68503 ,","place":["United States"]},{"name":"Nebraska Center for Biotechnology, University of Nebraska-Lincoln , Lincoln, NE 68503 ,","place":["United States"]},{"name":"Nebraska Center for Biomolecular Communication (CIBC), University of Nebraska-Lincoln , Lincoln, NE 68508 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigar D","family":"Babayeva","sequence":"additional","affiliation":[{"name":"Eppley Institute for Research in Cancer and Allied Diseases, Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center , Omaha, NE 68198 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1238-0069","authenticated-orcid":false,"given":"Tahir\u00a0H","family":"Tahirov","sequence":"additional","affiliation":[{"name":"Eppley Institute for Research in Cancer and Allied Diseases, Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center , Omaha, NE 68198 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,6,12]]},"reference":[{"key":"2026061204202236500_B1","doi-asserted-by":"publisher","first-page":"22766","DOI":"10.3402\/jom.v5i0.22766","article-title":"Herpesviruses: latency and reactivation \u2013 viral strategies and host response","volume":"5","author":"Grinde","year":"2013","journal-title":"J Oral 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