{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:53:06Z","timestamp":1790668386322,"version":"4.1.0"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"vor","delay-in-days":10,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Bacteriophage exclusion (BREX) defense systems restrict phage infection via inhibition of phage DNA replication, while also modifying and protecting the bacterial genome. Type I BREX systems encode six conserved proteins, including a site-specific DNA methyltransferase. Host methylation requires a subset of BREX proteins, whereas phage restriction generally requires them all, suggesting that distinct but overlapping complexes mediate these activities. Full details of the mechanism and regulation of BREX remain to be understood. Here, we characterize the behavior and structures of the conserved BrxC AAA+ ATPase protein. BrxC forms multiple assemblages\u2014various self-associating multimers, as well as a complex with BrxB-PglZ\u2014that can be uncoupled via distinct point mutations, leading to differing effects on host methylation versus phage restriction. BrxC\u2019s self-association, as well as its ability to bind DNA, is regulated by ATP binding and hydrolysis; BrxA and BrxB appear to also regulate those behaviors. These collective results suggest that BrxC may play a key role in controlling the two activities of BREX, with BrxB, BrxC, and PglZ forming a core complex, and the equilibrium among competing assemblies containing those proteins modulating the balance between idling and activated restrictive states.<\/jats:p>","DOI":"10.1093\/nar\/gkag651","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:35:01Z","timestamp":1782866101000},"source":"Crossref","is-referenced-by-count":1,"title":["Multiple forms of protein\u2013protein and DNA binding are exhibited by BrxC from the BREX phage restriction system"],"prefix":"10.1093","volume":"54","author":[{"given":"Alexander J","family":"Kaiser","sequence":"first","affiliation":[{"name":"Division of Basic Sciences, Fred Hutchinson Cancer Center , 1100 Fairview Ave. N., Seattle, WA 98109 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer 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