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The Ku70\/Ku80 heterodimer (Ku) initiates NHEJ by encircling dsDNA ends and recruiting DNA-PKcs. Ku70 in plants and mammals acquired a C-terminal SAP domain implicated in nucleic acid binding. Here, we show that in murine models, the SAP domain is dispensable for Ku stability and recruitment to DNA breaks. Unlike\u00a0Ku70\u2212\/\u2212 mice, Ku70\u0394SAP\/\u0394SAP mice exhibit normal lymphocyte development despite mild radiation sensitivity. Structural modeling places the SAP domain in adjacent DNA grooves, where it can restrict Ku\u2019s lateral movement along dsDNA. Correspondingly, in mice lacking DNA-PKcs that caps the ends, Ku70\u0394SAP reduces T cell counts and deletion sizes, consistent with Ku translocating off DNA. Moreover, SAP deletion reduced DNA-end affinity, increased dissociation, and exchange of purified Ku at low concentrations, and increased multiple-loading at high concentrations, consistent with increased lateral movement. In DNA-PKcs\u2212\/\u2212 murine fibroblasts, deletion or lysine mutation (K593\/4A, corresponding to K595\/6A in human Ku70) in the SAP domain decreased the relative intensity of laser-induced Ku spots, revealing a role of the SAP domain in constraining Ku lateral movement on dsDNA in the absence of DNA-PKcs (or in the short-range complex).<\/jats:p>","DOI":"10.1093\/nar\/gkag670","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T11:15:17Z","timestamp":1783595717000},"source":"Crossref","is-referenced-by-count":1,"title":["Ku70-SAP domain has an overlapping function with DNA-PKcs in limiting the lateral movement of the Ku ring along DNA"],"prefix":"10.1093","volume":"54","author":[{"given":"Yimeng","family":"Zhu","sequence":"first","affiliation":[{"name":"Institute for Cancer Genetics, Vagelos College of Physicians and Surgeons, Columbia University , New York, NY 10032 ,","place":["United 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for Cancer Genetics, Vagelos College of Physicians and Surgeons, Columbia University , New York, NY 10032 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shingo","family":"Fujii","sequence":"additional","affiliation":[{"name":"Department of Genome Integrity, Cancer Research Center of Marseille, CNRS UMR7258, Inserm U1068, Institut Paoli-Calmettes, Aix Marseille University , 13273 Marseille ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelina","family":"Li","sequence":"additional","affiliation":[{"name":"Institute for Cancer Genetics, Vagelos College of Physicians and Surgeons, Columbia University , New York, NY 10032 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duc-Duy","family":"Vu","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Chimie, LBM, CNRS UMR 7203, \u00c9cole Normale Sup\u00e9rieure, PSL University, Sorbonne University , 75005 Paris ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle J","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute for Cancer Genetics, Vagelos College of Physicians and Surgeons, Columbia University , New York, NY 10032 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eli","family":"Rothenberg","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Pharmacology, School of Medicine, New York University , New York, NY 10016 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4964-331X","authenticated-orcid":false,"given":"Mauro","family":"Modesti","sequence":"additional","affiliation":[{"name":"Department of Genome Integrity, Cancer Research Center of Marseille, CNRS UMR7258, Inserm U1068, Institut Paoli-Calmettes, Aix Marseille University , 13273 Marseille 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PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair","volume":"347","author":"Ochi","year":"2015","journal-title":"Science"},{"key":"2026081001112721900_B6","doi-asserted-by":"publisher","first-page":"6233","DOI":"10.1038\/ncomms7233","article-title":"Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway","volume":"6","author":"Xing","year":"2015","journal-title":"Nat Commun"},{"key":"2026081001112721900_B7","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1101\/gad.353407.125","article-title":"Nonhomologous end joining-the importance of end tethering and beyond","volume":"40","author":"Zha","year":"2026","journal-title":"Genes Dev"},{"key":"2026081001112721900_B8","doi-asserted-by":"publisher","first-page":"18597","DOI":"10.1073\/pnas.0609061103","article-title":"Dynamic assembly of end-joining complexes requires interaction between Ku70\/80 and 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