{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:59:10Z","timestamp":1785427150512,"version":"3.56.0"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"14","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":13,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35NS111631"],"award-info":[{"award-number":["R35NS111631"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["S10 OD030335"],"award-info":[{"award-number":["S10 OD030335"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RM1 HG011563"],"award-info":[{"award-number":["RM1 HG011563"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Efficient purification of specific RNAs from lysates remains a major challenge. Existing methods, such as MS2 tagging or oligonucleotide hybridization, require protein immobilization or sequence-specific hybridization, limiting scalability and compatibility with diverse RNAs. Current approaches often have low recovery, exhibit slow kinetics, and generate background contamination, limiting their use in RNA\u2013protein interaction studies. To overcome these limitations, we developed FS2, an RNA sequence that binds Sephadex beads with high affinity, thus acting as an affinity tag for protein-free, rapid, and simple RNA purification. FS2 consists of two copies of the dextran-binding D8 aptamer embedded within the highly stable F30 three-way junction RNA scaffold, which promotes aptamer folding and enhances avidity. Systematic optimization revealed that FS2 exhibits rapid binding kinetics, efficient purification, and efficient elution under mild conditions (50\u00b0C, 10 mM EDTA), representing substantial improvements over existing RNA purification systems. We validated the utility of FS2 by purifying an FS2-tagged MYC mRNA fragment from HEK293T cells and demonstrating that N6-methyladenosine (m6A)-containing mRNAs can be pulled down along with the m6A-binding protein YTHDF2. Overall, FS2 provides a highly simple and efficient RNA-based affinity tag for recovering RNA from complex mixtures and lysates for diverse applications.<\/jats:p>","DOI":"10.1093\/nar\/gkag735","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T11:25:19Z","timestamp":1783941919000},"source":"Crossref","is-referenced-by-count":0,"title":["A multivalent RNA affinity tag enables selective purification of tagged RNAs and bound proteins"],"prefix":"10.1093","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2636-1675","authenticated-orcid":false,"given":"Ki Sung","family":"Park","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Weill Cornell Medicine, Cornell University , New York, NY 10065 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sourav Kumar","family":"Dey","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Weill Cornell Medicine, Cornell 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