{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T08:01:39Z","timestamp":1784966499315,"version":"3.55.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"14","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":8,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82372351"],"award-info":[{"award-number":["82372351"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82572673"],"award-info":[{"award-number":["82572673"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005230","name":"Natural Science Foundation of Chongqing","doi-asserted-by":"publisher","award":["CSTB2024NSCQ-MSX0521"],"award-info":[{"award-number":["CSTB2024NSCQ-MSX0521"]}],"id":[{"id":"10.13039\/501100005230","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005230","name":"Natural Science Foundation of Chongqing","doi-asserted-by":"publisher","award":["CSTB2024NSCQ-MSX1223"],"award-info":[{"award-number":["CSTB2024NSCQ-MSX1223"]}],"id":[{"id":"10.13039\/501100005230","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2024M763898"],"award-info":[{"award-number":["2024M763898"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chongqing Postdoctoral Special Funding Project","award":["2024CQBSHTB3005"],"award-info":[{"award-number":["2024CQBSHTB3005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>CRISPR\u2013Cas13a is mainly known as an RNA-guided RNA endonuclease. Recent studies show that Leptotrichia buccalis Cas13a (LbuCas13a) can interact with DNA substrates too, without PAM or PFS constraints, but current understanding of DNA-mediated activation is largely based on continuous target strands. Here, we define a structural accessibility principle for LbuCas13a activation by noncontiguous DNA. We show that activation occurs only when overhang positioning creates an accessible protein\u2013DNA interface. Outer overhangs near the crRNA repeat-adjacent side restore strong trans-cleavage activity by stabilizing key LbuCas13a\u2013DNA contacts, whereas distal outer overhangs support only weak activation. In contrast, inner overhangs cause steric mismatch, destabilize the complex, and block formation of an active conformation. Molecular modeling and molecular dynamics simulations support this structure-dependent rule. Noncontiguous DNA also broadens the single-nucleotide discrimination window of LbuCas13a and enables accurate IDH1 R132H detection in glioma tissues. We further develop a one-step APE1-activated CRISPR\u2013LbuCas13a reaction (ACROSS) for sensitive APE1 detection. Because activated LbuCas13a cleaves RNA reporters but not DNA-triggering products, ACROSS preserves the activating structure and supports stable signaling in vitro, in live cells, and in breast cancer serum samples.<\/jats:p>","DOI":"10.1093\/nar\/gkag740","type":"journal-article","created":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T07:57:27Z","timestamp":1784966247000},"source":"Crossref","is-referenced-by-count":0,"title":["A structural accessibility principle for LbuCas13a activation by noncontiguous DNA"],"prefix":"10.1093","volume":"54","author":[{"given":"Weitao","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University , No.1 Youyi Road, Chongqing 400016 ,","place":["P.R. China"]},{"name":"Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016 ,","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhan","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016 ,","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyun","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016 ,","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016 ,","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Gui","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016 ,","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016 ,","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Yin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016 ,","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaole","family":"Han","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016 ,","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016 ,","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiling","family":"Lu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016 ,","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016 ,","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"The Center for Clinical Molecular Medical Detection, Engineering Research Center of Chongqing Education Commission of China for IVD Technology Innovation and Translation, Laboratory Medicine Center, The First Affiliated Hospital of Chongqing Medical University , Chongqing 400016 ,","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3340-7133","authenticated-orcid":false,"given":"Guoming","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University , No.1 Youyi Road, Chongqing 400016 ,","place":["P.R. China"]},{"name":"Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016 ,","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"2026072503571941400_B1","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1038\/nature15386","article-title":"CRISPR\u2013Cas immunity in prokaryotes","volume":"526","author":"Marraffini","year":"2015","journal-title":"Nature"},{"key":"2026072503571941400_B2","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1016\/j.molcel.2015.10.008","article-title":"Discovery and functional characterization of diverse class 2 CRISPR\u2013Cas systems","volume":"60","author":"Shmakov","year":"2015","journal-title":"Mol Cell"},{"key":"2026072503571941400_B3","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1038\/nrmicro.2016.184","article-title":"Diversity and evolution of class 2 CRISPR\u2013Cas systems","volume":"15","author":"Shmakov","year":"2017","journal-title":"Nat Rev Microbiol"},{"key":"2026072503571941400_B4","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1038\/nature19802","article-title":"Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection","volume":"538","author":"East-Seletsky","year":"2016","journal-title":"Nature"},{"key":"2026072503571941400_B5","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1126\/science.aam9321","article-title":"Nucleic acid detection with CRISPR\u2013Cas13a\/C2c2","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"key":"2026072503571941400_B6","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1016\/j.molcel.2019.10.024","article-title":"Dynamic imaging of RNA in living cells by CRISPR\u2013Cas13 systems","volume":"76","author":"Yang","year":"2019","journal-title":"Mol Cell"},{"key":"2026072503571941400_B7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41589-025-02136-3","article-title":"De novo design of potent CRISPR\u2013Cas13 inhibitors","author":"Taveneau","year":"2026","journal-title":"Nat Chem Biol"},{"key":"2026072503571941400_B8","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1038\/nature24049","article-title":"RNA targeting with CRISPR\u2013Cas13","volume":"550","author":"Abudayyeh","year":"2017","journal-title":"Nature"},{"key":"2026072503571941400_B9","doi-asserted-by":"publisher","first-page":"1301","DOI":"10.1126\/science.aax7852","article-title":"CRISPR-mediated live imaging of genome editing and transcription","volume":"365","author":"Wang","year":"2019","journal-title":"Science"},{"key":"2026072503571941400_B10","doi-asserted-by":"publisher","first-page":"3005","DOI":"10.1016\/j.scib.2025.07.015","article-title":"RNA target-independent non-canonical activation (RINCA) of Cas13 trans-nuclease activity","volume":"70","author":"Liu","year":"2025","journal-title":"Sci Bull"},{"key":"2026072503571941400_B11","doi-asserted-by":"publisher","first-page":"906","DOI":"10.1093\/nar\/gkad1127","article-title":"Unveiling the RNA-mediated allosteric activation discloses functional hotspots in CRISPR\u2013Cas13a","volume":"52","author":"Sinha","year":"2024","journal-title":"Nucleic Acids Res"},{"key":"2026072503571941400_B12","doi-asserted-by":"publisher","first-page":"2141","DOI":"10.1038\/s41551-025-01424-6","article-title":"LbuCas13a directly targets DNA and elicits strong trans-cleavage activity","volume":"9","author":"Wu","year":"2025","journal-title":"Nat Biomed Eng"},{"key":"2026072503571941400_B13","doi-asserted-by":"publisher","first-page":"117760","DOI":"10.1016\/j.bios.2025.117760","article-title":"Single-tube lambda exonuclease-mediated LbuCas13a detect of ssDNA for single-nucleotide polymorphisms genotyping","volume":"288","author":"Luo","year":"2025","journal-title":"Biosens Bioelectron"},{"key":"2026072503571941400_B14","doi-asserted-by":"publisher","first-page":"866","DOI":"10.1126\/science.aat5011","article-title":"CRISPR\u2013Cas guides the future of genetic engineering","volume":"361","author":"Knott","year":"2018","journal-title":"Science"},{"key":"2026072503571941400_B15","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1038\/s41592-021-01124-4","article-title":"Programmable RNA editing with compact CRISPR\u2013Cas13 systems from uncultivated microbes","volume":"18","author":"Xu","year":"2021","journal-title":"Nat Methods"},{"key":"2026072503571941400_B16","doi-asserted-by":"publisher","first-page":"1967","DOI":"10.1038\/s41564-022-01258-x","article-title":"Broad-spectrum CRISPR\u2013Cas13a enables efficient phage genome editing","volume":"7","author":"Adler","year":"2022","journal-title":"Nat Microbiol"},{"key":"2026072503571941400_B17","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1038\/s41592-020-0866-0","article-title":"CRISPR-assisted detection of RNA\u2013protein interactions in living cells","volume":"17","author":"Yi","year":"2020","journal-title":"Nat Methods"},{"key":"2026072503571941400_B18","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1038\/s41586-025-09570-2","article-title":"Heat-rechargeable computation in DNA logic circuits and neural networks","volume":"646","author":"Song","year":"2025","journal-title":"Nature"},{"key":"2026072503571941400_B19","doi-asserted-by":"publisher","first-page":"4722","DOI":"10.1093\/nar\/gkq240","article-title":"WRN helicase unwinds Okazaki fragment-like hybrids in a reaction stimulated by the human DHX9 helicase","volume":"38","author":"Chakraborty","year":"2010","journal-title":"Nucleic Acids Res"},{"key":"2026072503571941400_B20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41587-025-02868-6","article-title":"RNA structure modulates Cas13 activity and enables mismatch detection","author":"Larsen","year":"2025","journal-title":"Nat Biotechnol"},{"key":"2026072503571941400_B21","doi-asserted-by":"publisher","first-page":"2901","DOI":"10.1038\/s41467-024-47281-w","article-title":"The CRISPR\u2013Cas13a Gemini System for noncontiguous target RNA activation","volume":"15","author":"Zhao","year":"2024","journal-title":"Nat Commun"},{"key":"2026072503571941400_B22","doi-asserted-by":"publisher","first-page":"7384","DOI":"10.1093\/nar\/gkae470","article-title":"Modular CRISPR\/Cas12a synergistic activation platform for detection and logic operations","volume":"52","author":"Hu","year":"2024","journal-title":"Nucleic Acids Res"},{"key":"2026072503571941400_B23","doi-asserted-by":"publisher","first-page":"11148","DOI":"10.1093\/nar\/gkae766","article-title":"Synergistic effect of split DNA activators of Cas12a with exon-unwinding and induced targeting effect","volume":"52","author":"Huang","year":"2024","journal-title":"Nucleic Acids Res"},{"key":"2026072503571941400_B24","doi-asserted-by":"publisher","first-page":"gkaf117","DOI":"10.1093\/nar\/gkaf117","article-title":"Regulation of CRISPR trans-cleavage activity by an overhanging activator","volume":"53","author":"Yin","year":"2025","journal-title":"Nucleic Acids Res"},{"key":"2026072503571941400_B25","doi-asserted-by":"publisher","first-page":"921","DOI":"10.1093\/nar\/gkad1132","article-title":"New design strategies for ultra-specific CRISPR\u2013Cas13a-based RNA detection with single-nucleotide mismatch sensitivity","volume":"52","author":"Molina\u00a0Vargas","year":"2024","journal-title":"Nucleic Acids Res"},{"key":"2026072503571941400_B26","doi-asserted-by":"publisher","first-page":"9826","DOI":"10.1021\/acs.analchem.2c01670","article-title":"Enzyme kinetics and detector sensitivity determine limits of detection of amplification-free CRISPR\u2013Cas12 and CRISPR\u2013Cas13 diagnostics","volume":"94","author":"Huyke","year":"2022","journal-title":"Anal Chem"},{"key":"2026072503571941400_B27","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/j.cell.2020.12.001","article-title":"Amplification-free detection of SARS-CoV-2 with CRISPR\u2013Cas13a and mobile phone microscopy","volume":"184","author":"Fozouni","year":"2021","journal-title":"Cell"},{"key":"2026072503571941400_B28","doi-asserted-by":"publisher","first-page":"eadl0731","DOI":"10.1126\/sciadv.adl0731","article-title":"Principles of CRISPR\u2013Cas13 mismatch intolerance enable selective silencing of point-mutated oncogenic RNA with single-base precision","volume":"10","author":"Shembrey","year":"2024","journal-title":"Sci Adv"},{"key":"2026072503571941400_B29","doi-asserted-by":"publisher","first-page":"gkag161","DOI":"10.1093\/nar\/gkag161","article-title":"A novel dual-guide CRISPR\u2013Cas13 strategy improves specificity for single-nucleotide variant detection","volume":"54","author":"Aguilar-Gonz\u00e1lez","year":"2026","journal-title":"Nucleic Acids Res"},{"key":"2026072503571941400_B30","doi-asserted-by":"publisher","first-page":"e06716","DOI":"10.1002\/advs.202506716","article-title":"One-pot isothermal nucleic acid amplification assisted CRISPR\/Cas detection technology: challenges, strategies, and perspectives","volume":"12","author":"Liu","year":"2025","journal-title":"Adv Sci"},{"key":"2026072503571941400_B31","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1016\/j.tibtech.2024.07.007","article-title":"Trends in developing one-pot CRISPR diagnostics strategies","volume":"43","author":"Chen","year":"2025","journal-title":"Trends Biotechnol"},{"key":"2026072503571941400_B32","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1016\/j.trecan.2018.05.006","article-title":"Applications of CRISPR\u2013Cas enzymes in cancer therapeutics and detection","volume":"4","author":"Huang","year":"2018","journal-title":"Trends Cancer"},{"key":"2026072503571941400_B33","doi-asserted-by":"publisher","first-page":"5390","DOI":"10.1038\/s41467-022-33057-7","article-title":"Structural basis for APE1 processing DNA damage in the nucleosome","volume":"13","author":"Weaver","year":"2022","journal-title":"Nat Commun"},{"key":"2026072503571941400_B34","doi-asserted-by":"publisher","first-page":"4711","DOI":"10.1093\/nar\/gki781","article-title":"Isolation of a small molecule inhibitor of DNA base excision repair","volume":"33","author":"Madhusudan","year":"2005","journal-title":"Nucleic Acids Res"},{"key":"2026072503571941400_B35","doi-asserted-by":"publisher","first-page":"e202417165","DOI":"10.1002\/anie.202417165","article-title":"Spherical DNA nanomotors enable ultrasensitive detection of active enzymes in extracellular vesicles for cancer diagnosis","volume":"64","author":"Deng","year":"2025","journal-title":"Angew Chem Int Ed Engl"},{"key":"2026072503571941400_B36","doi-asserted-by":"publisher","first-page":"4695","DOI":"10.1039\/D4CC00304G","article-title":"Enhancing APE1 detection through apurinic\/apyrimidinic site inhibition of DNA polymerase: an innovative, highly sensitive approach","volume":"60","author":"Liu","year":"2024","journal-title":"Chem Commun"},{"key":"2026072503571941400_B37","doi-asserted-by":"publisher","first-page":"1312","DOI":"10.1038\/s42003-024-07000-z","article-title":"Harnessing noncanonical trans-cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnostics","volume":"7","author":"Wu","year":"2024","journal-title":"Commun Biol"},{"key":"2026072503571941400_B38","doi-asserted-by":"publisher","first-page":"5845","DOI":"10.1038\/s41467-023-41501-5","article-title":"Structural basis for the activation of a compact CRISPR\u2013Cas13 nuclease","volume":"14","author":"Deng","year":"2023","journal-title":"Nat Commun"},{"key":"2026072503571941400_B39","doi-asserted-by":"publisher","first-page":"111226","DOI":"10.1016\/j.celrep.2022.111226","article-title":"Negative autoregulation mitigates collateral RNase activity of repeat-targeting CRISPR\u2013Cas13d in mammalian cells","volume":"40","author":"Kelley","year":"2022","journal-title":"Cell Rep"},{"key":"2026072503571941400_B40","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1038\/s41564-022-01318-2","article-title":"Restriction endonuclease cleavage of phage DNA enables resuscitation from Cas13-induced bacterial dormancy","volume":"8","author":"Williams","year":"2023","journal-title":"Nat Microbiol"},{"key":"2026072503571941400_B41","doi-asserted-by":"publisher","first-page":"gkaf596","DOI":"10.1093\/nar\/gkaf596","article-title":"PAM-free hairpin target binding activates trans-cleavage activity of Cas12a","volume":"53","author":"Li","year":"2025","journal-title":"Nucleic Acids Res"},{"key":"2026072503571941400_B42","doi-asserted-by":"publisher","first-page":"gkaf311","DOI":"10.1093\/nar\/gkaf311","article-title":"Exploring the effect of activator topology on CRISPR\u2013Cas12a trans-cleavage activity","volume":"53","author":"Zhu","year":"2025","journal-title":"Nucleic Acids Res"},{"key":"2026072503571941400_B43","doi-asserted-by":"publisher","first-page":"e202515521","DOI":"10.1002\/anie.202515521","article-title":"Allosteric activation of Cas12a via PAM topological engineering for direct and rapid detection of nucleases","volume":"64","author":"Tao","year":"2025","journal-title":"Angew Chem Int Ed"},{"key":"2026072503571941400_B44","doi-asserted-by":"publisher","first-page":"1818","DOI":"10.1038\/s41467-024-46001-8","article-title":"Topological barrier to Cas12a activation by circular DNA nanostructures facilitates autocatalysis and transforms DNA\/RNA sensing","volume":"15","author":"Deng","year":"2024","journal-title":"Nat Commun"},{"key":"2026072503571941400_B45","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1038\/nmeth.3733","article-title":"Editing the epigenome: technologies for programmable transcription and epigenetic modulation","volume":"13","author":"Thakore","year":"2016","journal-title":"Nat Methods"},{"key":"2026072503571941400_B46","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1038\/s41467-024-44867-2","article-title":"Programmable RNA base editing with photoactivatable CRISPR\u2013Cas13","volume":"15","author":"Yu","year":"2024","journal-title":"Nat Commun"},{"key":"2026072503571941400_B47","doi-asserted-by":"publisher","first-page":"5561","DOI":"10.1021\/jacs.3c01087","article-title":"A split CRISPR\/Cas13b system for conditional RNA regulation and editing","volume":"145","author":"Xu","year":"2023","journal-title":"J Am Chem Soc"}],"container-title":["Nucleic Acids Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/nar\/article-pdf\/54\/14\/gkag740\/70009230\/gkag740.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/nar\/article-pdf\/54\/14\/gkag740\/70009230\/gkag740.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T07:57:28Z","timestamp":1784966248000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/nar\/article\/doi\/10.1093\/nar\/gkag740\/8741783"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,17]]},"references-count":47,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2026,7,17]]}},"URL":"https:\/\/doi.org\/10.1093\/nar\/gkag740","relation":{},"ISSN":["0305-1048","1362-4962"],"issn-type":[{"value":"0305-1048","type":"print"},{"value":"1362-4962","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,8,12]]},"published":{"date-parts":[[2026,7,17]]},"article-number":"gkag740"}}