{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T02:10:18Z","timestamp":1791511818584,"version":"4.3.4"},"reference-count":73,"publisher":"Oxford University Press (OUP)","issue":"14","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":18,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DP2GM149750"],"award-info":[{"award-number":["DP2GM149750"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001368","name":"V Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001368","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Pew Biomedical Scholars"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A cell\u2019s fate is shaped by its inherited state, or lineage, and the ever-shifting context of its environment. CRISPR-based recording technologies are a promising solution for mapping the lineage of a developing system; however, challenges remain regarding single-cell recovery, engineering complexity, and scale. Here, we introduce BASELINE, which uses base editing to generate high-resolution lineage trees in conjunction with single-cell profiling. BASELINE uses the Cas12a adenine base editor to irreversibly edit nucleotides across target arrays built from 50 synthetic target sites, which are integrated multiple times into a cell\u2019s genome. We demonstrate that BASELINE accumulates lineage-specific marks over a wide range of biologically relevant intervals, recording more than 4300 bits of information in a model of pancreatic cancer, a 50-fold increase over existing technologies. Single-cell sequencing reveals high-fidelity capture of these recorders, averaging 29 cell divisions captured per lineage, within the estimated range of mammalian development. We expect BASELINE to apply to a wide range of lineage-tracing projects in development and disease, especially those in which cellular engineering makes small, more distributed systems challenging.<\/jats:p>","DOI":"10.1093\/nar\/gkag769","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T11:38:52Z","timestamp":1784893132000},"source":"Crossref","is-referenced-by-count":4,"title":["BASELINE: a CRISPR base editing platform for mammalian-scale single-cell lineage tracing"],"prefix":"10.1093","volume":"54","author":[{"given":"Evan","family":"Winter","sequence":"first","affiliation":[{"name":"Molecular and Systems Biology, Dartmouth College , Hanover, NH, 03755 ,","place":["USA"]},{"name":"Molecular and Cellular Biology Program, Dartmouth College , Hanover, NH, 03756 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Emiliani","sequence":"additional","affiliation":[{"name":"Molecular and Systems Biology, Dartmouth College , Hanover, NH, 03755 ,","place":["USA"]},{"name":"Molecular and Cellular Biology Program, Dartmouth College , Hanover, NH, 03756 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aidan","family":"Cook","sequence":"additional","affiliation":[{"name":"Molecular and Systems Biology, Dartmouth College , Hanover, NH, 03755 ,","place":["USA"]},{"name":"Quantitative Biomedical Science Program, Dartmouth College , Lebanon, NH, 03756 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Abderrahim","sequence":"additional","affiliation":[{"name":"POWERED Program, Dartmouth Cancer Center, Dartmouth College , Lebanon, NH, 03756 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8277-6512","authenticated-orcid":false,"given":"Aaron","family":"McKenna","sequence":"additional","affiliation":[{"name":"Molecular and Systems Biology, Dartmouth College , Hanover, NH, 03755 ,","place":["USA"]},{"name":"Dartmouth Cancer Center, Dartmouth College , Lebanon, NH, 03755 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,4]]},"reference":[{"key":"2026080404430151500_B1","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.cell.2012.01.002","article-title":"Lineage tracing","volume":"148","author":"Kretzschmar","year":"2012","journal-title":"Cell"},{"key":"2026080404430151500_B2","first-page":"1","article-title":"The organization and cell-lineage of the ascidian egg","volume":"13","author":"Conklin","year":"1905","journal-title":"J Acad Nat Sci Phila"},{"key":"2026080404430151500_B3","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1016\/0012-1606(83)90201-4","article-title":"The embryonic cell lineage of the nematode Caenorhabditis elegans","volume":"100","author":"Sulston","year":"1983","journal-title":"Dev 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