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Here, we present Antigen Affinity BCR-seq (AAB-seq), a high-throughput single-cell sequencing platform that can obtain the relative antibody\u2013antigen affinity of thousands of paired native BCR sequences. AAB-seq employs dual-labeled antigens and DNA-barcoded anti-light-chain antibodies to compute an AAB score that is proportional to antibody\u2013antigen binding strength. Integrated with a rapid, low-cost direct cloning workflow, it enables affinity-guided antibody retrieval without de novo antibody gene synthesis. Validated against ovalbumin and SARS\u2013COV\u20132 RBD, AAB-seq discovered potent antibodies whose AAB score correlates strongly with ELISA, including novel SARS\u2013COV\u20132 neutralizing antibodies with potent effector functions. 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