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Despite its well-established function in the animal germline, piRNAs and PIWI proteins are expressed in somatic tissues across arthropod species, and their functions outside the gonads remain poorly understood.\u00a0Aedes albopictus mosquitoes express four PIWI genes, Piwi4, Piwi5, Piwi6, and\u00a0Ago3, in both gonadal and somatic tissues. Here, we generated Piwi6 knockout (KO) Ae. albopictus cell lines and observed a substantial upregulation of long terminal repeat retrotransposons, including a full-length endogenous retrovirus that we named Aedes albopictus Endogenous Retrovirus-1 (AalERV1). Nascent RNA sequencing and Cleavage Under Targets and Tagmentation (CUT&amp;Tag) analyses revealed that Piwi6 silences\u00a0AalERV1 transcriptionally by guiding the deposition of the repressive H3K9me3 histone mark. Consistently, Piwi6 localized to both the cytoplasm and nucleus, with sequences in the intrinsically disordered region guiding nuclear translocation. Reintroduction of full-length GFP-Piwi6, but not a mutant GFP-Piwi6 defective in nuclear localization, rescued AalERV1 repression in Piwi6 KO cells. Importantly, Piwi6-mediated control of AalERV1 was recapitulated in vivo as Piwi6 knockdown increased AalERV1 expression in both ovaries and somatic tissues of Ae. albopictus mosquitoes. These results establish Aedes mosquitoes as a model to study nuclear PIWI functions and suggest that somatic piRNA-mediated transposon silencing is evolutionarily conserved across arthropod species.<\/jats:p>","DOI":"10.1093\/nar\/gkag834","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T11:34:42Z","timestamp":1786448082000},"source":"Crossref","is-referenced-by-count":0,"title":["The piRNA pathway mediates transcriptional silencing of LTR retrotransposons in ovaries and somatic tissues of\u00a0\n                    <i>Aedes<\/i>\n                    mosquitoes"],"prefix":"10.1093","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0427-2874","authenticated-orcid":false,"given":"Ezgi","family":"Ta\u015fk\u00f6pr\u00fc","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Center , 6525 GANijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nynke B","family":"van\u00a0Eijk","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Center , 6525 GANijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Femke A H","family":"van\u00a0Hout","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Center , 6525 GANijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianna","family":"Bacchi","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Center , 6525 GANijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8356-8165","authenticated-orcid":false,"given":"Gijs J","family":"Overheul","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Center , 6525 GANijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Linthout","sequence":"additional","affiliation":[{"name":"Laboratory of Entomology, Wageningen University and Research , 6708 PBWageningen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constantianus J M","family":"Koenraadt","sequence":"additional","affiliation":[{"name":"Laboratory of Entomology, Wageningen University and Research , 6708 PBWageningen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieqiong","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Center , 6525 GANijmegen ,","place":["The 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