{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T09:02:35Z","timestamp":1789722155299,"version":"4.0.1"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"17","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":11,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Excellence Strategy of the German Federal"},{"DOI":"10.13039\/100010663","name":"European Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Union's Horizon"},{"name":"Consolidator Grant GemOmics","award":["101044142"],"award-info":[{"award-number":["101044142"]}]},{"name":"Consolidator Grant GemOmics","award":["PRO-ZHOU-2023-20"],"award-info":[{"award-number":["PRO-ZHOU-2023-20"]}]},{"DOI":"10.13039\/501100002345","name":"University of T\u00fcbingen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002345","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Membraneless organelles (MLOs) in eukaryotic cells, such as the nuclear Cajal body (CB), have been notoriously difficult to biochemically isolate due to their dynamic and delicate nature. CBs, formed via liquid\u2013liquid phase separation, mediate RNA processing and ribonucleoprotein assembly, essential for nuclear functions. In plants, this challenge has prevented any direct molecular characterization to date. Here, we report a successful isolation of plant CBs, achieved through a combination of biochemical purification and fluorescence-activated nuclei and particle sorting, enabling a previously unattainable biochemical access to this organelle. Mass spectrometry analysis defined a high-confidence set of plant CB-associated proteins, identifying 15 and 79 proteins uniquely localized and enriched in CBs, respectively, of which 18 are also labelled by CB markers in proximity labelling assays. While a partial overlap with known animal CB proteins exists, our data suggests a partially divergent composition of CB-associated proteins across kingdoms. This work breaks a major technical challenge, enabling detailed characterization of plant CBs and establishing a versatile platform adaptable for the isolation and molecular dissection of other MLOs.<\/jats:p>","DOI":"10.1093\/nar\/gkag867","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T08:16:31Z","timestamp":1789719391000},"source":"Crossref","is-referenced-by-count":0,"title":["Proteome profiling of plant Cajal bodies uncovers kingdom-specific components"],"prefix":"10.1093","volume":"54","author":[{"given":"Yu","family":"Zhou","sequence":"first","affiliation":[{"name":"Plant Biochemistry Department, Center for Plant Molecular Biology (ZMBP), Eberhard Karls University T\u00fcbingen , T\u00fcbingen,D-72076 ,","place":["Germany"]},{"name":"Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University,\u00a0518060 , Shenzhen ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huang","family":"Tan","sequence":"additional","affiliation":[{"name":"Plant Biochemistry Department, Center for Plant Molecular Biology (ZMBP), Eberhard Karls University T\u00fcbingen , T\u00fcbingen,D-72076 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4496-7091","authenticated-orcid":false,"given":"Axel","family":"Giudicatti","sequence":"additional","affiliation":[{"name":"Plant Biochemistry Department, Center for Plant Molecular Biology (ZMBP), Eberhard Karls University T\u00fcbingen , T\u00fcbingen,D-72076 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Vicente-Luque","sequence":"additional","affiliation":[{"name":"Plant Biochemistry Department, Center for Plant Molecular Biology (ZMBP), Eberhard Karls University T\u00fcbingen , T\u00fcbingen,D-72076 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marvin","family":"Weis","sequence":"additional","affiliation":[{"name":"Plant Biochemistry Department, Center for Plant Molecular Biology (ZMBP), Eberhard Karls University T\u00fcbingen , T\u00fcbingen,D-72076 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lena","family":"Wehinger","sequence":"additional","affiliation":[{"name":"Plant Biochemistry Department, Center for Plant Molecular Biology (ZMBP), Eberhard Karls University T\u00fcbingen , T\u00fcbingen,D-72076 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Schreidl","sequence":"additional","affiliation":[{"name":"Plant Biochemistry Department, Center for Plant Molecular Biology (ZMBP), Eberhard Karls University T\u00fcbingen , T\u00fcbingen,D-72076 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5491-7162","authenticated-orcid":false,"given":"Irina","family":"Droste-Borel","sequence":"additional","affiliation":[{"name":"Proteome Center T\u00fcbingen,\u00a0Eberhard Karls University T\u00fcbingen , T\u00fcbingen,\u00a0 D-72076 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Macek","sequence":"additional","affiliation":[{"name":"Proteome Center T\u00fcbingen,\u00a0Eberhard Karls University T\u00fcbingen , T\u00fcbingen,\u00a0 D-72076 ,","place":["Germany"]},{"name":"Cluster of Excellence GreenRobust,\u00a0Eberhard Karls University T\u00fcbingen, T\u00fcbingen , D-72076 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9870-6164","authenticated-orcid":false,"given":"Kenneth\u00a0Wayne","family":"Berendzen","sequence":"additional","affiliation":[{"name":"Plant Biochemistry Department, Center for Plant Molecular Biology (ZMBP), Eberhard Karls University T\u00fcbingen , T\u00fcbingen,D-72076 ,","place":["Germany"]},{"name":"Cluster of Excellence GreenRobust,\u00a0Eberhard Karls University T\u00fcbingen, T\u00fcbingen , D-72076 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7348-8842","authenticated-orcid":false,"given":"Rosa","family":"Lozano-Dur\u00e1n","sequence":"additional","affiliation":[{"name":"Plant Biochemistry Department, Center for Plant Molecular Biology (ZMBP), Eberhard Karls University T\u00fcbingen , T\u00fcbingen,D-72076 ,","place":["Germany"]},{"name":"Cluster of Excellence GreenRobust,\u00a0Eberhard Karls University T\u00fcbingen, T\u00fcbingen , D-72076 ,","place":["Germany"]},{"name":"Department of Plant Virology, Max Planck Institute for Plant Breeding Research , Cologne, D-50829,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"2026091804162654200_B1","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1038\/nrm1262","article-title":"The centennial of the Cajal body","volume":"4","author":"Gall","year":"2003","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2026091804162654200_B2","doi-asserted-by":"publisher","first-page":"2256036","DOI":"10.1080\/19491034.2023.2256036","article-title":"Coilin and cajal bodies","volume":"14","author":"Stan\u011bk","year":"2023","journal-title":"Nucleus"},{"key":"2026091804162654200_B3","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.devcel.2020.09.010","article-title":"Emerging roles for phase separation in 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