{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:08:08Z","timestamp":1788952088002,"version":"build-2803163510"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"17","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":2,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Chinese Institutes for Medical Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>DNA mismatch repair (MMR) maintains genomic stability, and defects in MMR genes such as MLH1 and MSH2 predispose to cancer. Unlike other MMR components, MLH1 has unexplained roles in development, as Mlh1-deficient male mice exhibit severe testicular hypoplasia and sterility. Here, we uncover that MLH1 regulates testis development through the Hippo-Yes-associated protein (YAP) pathway. MLH1 directly binds YAP via its C-terminal domain and the WW domains of YAP, competitively inhibiting LATS1-mediated YAP phosphorylation. This interaction stabilizes YAP by suppressing ubiquitination and promotes its nuclear translocation dependent on MLH1\u2019s nuclear localization signal. Additionally, MLH1 facilitates YAP\u2013TEAD complex formation, enabling expression of testicular development genes, including Wt1, Sox9, and Ctgf. These functions are independent of the MMR activity of MLH1. Mlh1-deficient mice show elevated YAP phosphorylation, reduced target gene expression, and impaired proliferation in developing testes. Pharmacological inhibition of the Hippo pathway kinases MST1\/2 partially rescues testis hypoplasia in Mlh1\u2212\/\u2212 mice. These findings establish MLH1 as a Hippo pathway regulator and resolve its long-standing role in male gonad development.<\/jats:p>","DOI":"10.1093\/nar\/gkag883","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:16:44Z","timestamp":1788520604000},"source":"Crossref","is-referenced-by-count":0,"title":["Mismatch repair protein MLH1 controls testis development by regulating the Hippo-YAP signaling pathway"],"prefix":"10.1093","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4691-8146","authenticated-orcid":false,"given":"Xueying","family":"Li","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory of Tumor Resistance Mechanism and Clinical Translation, Chinese Institutes for Medical Research, Beijing 100069,","place":["China"]},{"name":"Chinese Institute for Cancer Research, Chinese Institutes for Medical Research , Beijing 100069 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1964-2009","authenticated-orcid":false,"given":"Jiajun","family":"Yang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Tumor Resistance Mechanism and Clinical Translation, Chinese Institutes for Medical Research, Beijing 100069,","place":["China"]},{"name":"Chinese Institute for Cancer Research, Chinese Institutes for Medical Research , Beijing 100069 ,","place":["China"]},{"name":"School of Basic Medical Sciences, Capital Medical University , Beijing 100069 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9842-4578","authenticated-orcid":false,"given":"Guo-Min","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Tumor Resistance Mechanism and Clinical Translation, Chinese Institutes for Medical Research, Beijing 100069,","place":["China"]},{"name":"Chinese Institute for Cancer Research, Chinese Institutes for Medical Research , Beijing 100069 ,","place":["China"]},{"name":"Beijing Chao-Yang Hospital, Capital Medical University , Beijing 100020 ,","place":["China"]},{"name":"School of Basic Medical Sciences, Capital Medical University , Beijing 100069 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"2026090902195854600_B1","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1146\/annurev.biochem.74.082803.133243","article-title":"DNA mismatch repair","volume":"74","author":"Kunkel","year":"2005","journal-title":"Annu Rev Biochem"},{"key":"2026090902195854600_B2","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1038\/cr.2007.115","article-title":"Mechanisms and functions of DNA mismatch repair","volume":"18","author":"Li","year":"2008","journal-title":"Cell 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