{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T09:02:31Z","timestamp":1789722151998,"version":"4.0.1"},"reference-count":90,"publisher":"Oxford University Press (OUP)","issue":"17","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":11,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM133437"],"award-info":[{"award-number":["R35GM133437"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>DNA inter-strand crosslinks (ICLs) are highly cytotoxic lesions that require coordinated processing for repair. The RecQ4-family helicase Hrq1 promotes ICL repair in Saccharomyces cerevisiae, yet the mechanistic basis of its function remains unclear. Notably, the catalytically inactive hrq1-K318A allele confers greater sensitivity to ICL-inducing agents than deletion of HRQ1, suggesting a dominant-negative effect. To define the basis of this phenotype, we performed a genetic suppressor screen combined with biochemical and structural analyses. Spontaneous suppressors of hrq1-K318A sensitivity were overwhelmingly intragenic second-site mutations, many of which are predicted to destabilize the protein or impair its ability to bind DNA. In all cases, these mutations alleviated the dominant-negative repair defect. Biochemical characterization of representative mutants, including a rationally designed DNA-binding mutant, demonstrated that disruption of DNA binding suppresses hrq1-K318A toxicity even when protein stability is retained. These findings support a model in which DNA engagement by a catalytically inactive RecQ4-family helicase contributes to dominant-negative interference with DNA repair. More broadly, this work provides insight into how incomplete loss-of-function alleles of human RECQL4 may disrupt genome maintenance pathways.<\/jats:p>","DOI":"10.1093\/nar\/gkag903","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T11:51:59Z","timestamp":1789041119000},"source":"Crossref","is-referenced-by-count":0,"title":["DNA binding contributes to dominant-negative effects of a catalytically inactive RecQ4-family helicase"],"prefix":"10.1093","volume":"54","author":[{"suffix":"III","given":"Robert H","family":"Simmons","sequence":"first","affiliation":[{"name":"Molecular & Cellular Biochemistry Department, Indiana University , Bloomington, IN 47405 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faith E","family":"McDevitt","sequence":"additional","affiliation":[{"name":"Molecular & Cellular Biochemistry Department, Indiana University , Bloomington, IN 47405 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Hurlock","sequence":"additional","affiliation":[{"name":"Molecular & Cellular Biochemistry Department, Indiana University , Bloomington, IN 47405 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael E","family":"Kumcu","sequence":"additional","affiliation":[{"name":"Molecular & Cellular Biochemistry Department, Indiana University , Bloomington, IN 47405 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2807-0452","authenticated-orcid":false,"given":"Matthew L","family":"Bochman","sequence":"additional","affiliation":[{"name":"Molecular & Cellular Biochemistry Department, Indiana University , Bloomington, IN 47405 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"2026091804173441400_B1","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1146\/annurev-biochem-060713-035428","article-title":"Human RecQ helicases in DNA repair, recombination, and replication","volume":"83","author":"Croteau","year":"2014","journal-title":"Annu Rev Biochem"},{"key":"2026091804173441400_B2","doi-asserted-by":"publisher","first-page":"e147301","DOI":"10.1172\/JCI147301","article-title":"RECON syndrome is a genome instability disorder caused by mutations in the DNA helicase RECQL1","volume":"132","author":"Abu-Libdeh","year":"2022","journal-title":"J Clin Invest"},{"key":"2026091804173441400_B3","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1186\/1750-1172-5-2","article-title":"Rothmund-Thomson syndrome","volume":"5","author":"Larizza","year":"2010","journal-title":"Orphanet J Rare Dis"},{"key":"2026091804173441400_B4","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.mad.2004.09.011","article-title":"Pathways and functions of the Werner syndrome protein","volume":"126","author":"Lee","year":"2005","journal-title":"Mech Ageing Dev"},{"key":"2026091804173441400_B5","volume-title":"GeneReviews((R))","author":"Langer","year":"1993"},{"key":"2026091804173441400_B6","doi-asserted-by":"publisher","first-page":"106433","DOI":"10.1016\/j.bpc.2020.106433","article-title":"Mutations in conserved functional domains of human RecQ helicases are associated with diseases and cancer: a review","volume":"265","author":"Mojumdar","year":"2020","journal-title":"Biophys Chem"},{"key":"2026091804173441400_B7","doi-asserted-by":"publisher","first-page":"147","DOI":"10.20517\/jtgg.2021.60","article-title":"Enigmatic role of WRN-RECQL helicase in DNA repair and its implications in cancer","volume":"6","author":"Gupta","year":"2022","journal-title":"J Transl Genet Genom"},{"key":"2026091804173441400_B8","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1038\/s41586-024-07318-y","article-title":"Chemoproteomic discovery of a covalent allosteric inhibitor of WRN helicase","volume":"629","author":"Baltgalvis","year":"2024","journal-title":"Nature"},{"key":"2026091804173441400_B9","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1038\/s41586-024-07350-y","article-title":"Discovery of WRN inhibitor HRO761 with synthetic lethality in MSI cancers","volume":"629","author":"Ferretti","year":"2024","journal-title":"Nature"},{"key":"2026091804173441400_B10","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1038\/s41586-020-2769-8","article-title":"Repeat expansions confer WRN dependence in microsatellite-unstable cancers","volume":"586","author":"van\u00a0Wietmarschen","year":"2020","journal-title":"Nature"},{"key":"2026091804173441400_B11","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1038\/s41586-019-1103-9","article-title":"Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens","volume":"568","author":"Behan","year":"2019","journal-title":"Nature"},{"key":"2026091804173441400_B12","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1038\/s41586-019-1102-x","article-title":"WRN helicase is a synthetic lethal target in microsatellite unstable cancers","volume":"568","author":"Chan","year":"2019","journal-title":"Nature"},{"key":"2026091804173441400_B13","doi-asserted-by":"publisher","first-page":"569823","DOI":"10.2147\/ITT.S569823","article-title":"Upregulated BLM and RECQL4 in osteosarcoma: association with poor prognosis, immune cell infiltration, and inhibitory effects of sphingosine kinase 1 inhibitor II\/Pilaralisib","volume":"15","author":"Chen","year":"2026","journal-title":"Immunotargets Ther"},{"key":"2026091804173441400_B14","doi-asserted-by":"publisher","first-page":"24962","DOI":"10.1038\/s41598-024-75938-5","article-title":"Synergistic effects of bloom helicase (BLM) inhibitor AO\/854 with cisplatin in prostate cancer","volume":"14","author":"Ma","year":"2024","journal-title":"Sci Rep"},{"key":"2026091804173441400_B15","doi-asserted-by":"publisher","first-page":"983181","DOI":"10.3389\/fimmu.2022.983181","article-title":"The BLM helicase is a new therapeutic target in multiple myeloma involved in replication stress survival and drug resistance","volume":"13","author":"Ovejero","year":"2022","journal-title":"Front Immunol"},{"key":"2026091804173441400_B16","doi-asserted-by":"publisher","first-page":"14798","DOI":"10.3390\/ijms232314798","article-title":"Discovery of a novel Bloom\u2019s Syndrome protein (BLM) inhibitor suppressing growth and metastasis of prostate cancer","volume":"23","author":"Ma","year":"2022","journal-title":"Int J Mol Sci"},{"key":"2026091804173441400_B17","doi-asserted-by":"publisher","first-page":"560","DOI":"10.1038\/s41420-025-02834-w","article-title":"Novel mutations in the RECQL4 gene affect its helicase functions, interactions with the BLM helicase and chemotherapeutics-induced cell death","volume":"11","author":"Kaczmarczyk","year":"2025","journal-title":"Cell Death Discov"},{"key":"2026091804173441400_B18","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1159\/000516568","article-title":"Human RecQL4 as a novel molecular target for cancer therapy","volume":"161","author":"Balajee","year":"2021","journal-title":"Cytogenet Genome Res"},{"key":"2026091804173441400_B19","doi-asserted-by":"publisher","first-page":"e201800120","DOI":"10.26508\/lsa.201800120","article-title":"Chromosome alignment maintenance requires the MAP RECQL4, mutated in the Rothmund-Thomson syndrome","volume":"2","author":"Yokoyama","year":"2019","journal-title":"Life Sci Alliance"},{"key":"2026091804173441400_B20","doi-asserted-by":"publisher","first-page":"6632","DOI":"10.1093\/nar\/gks349","article-title":"The human RecQ helicases BLM and RECQL4 cooperate to preserve genome stability","volume":"40","author":"Singh","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"2026091804173441400_B21","doi-asserted-by":"publisher","first-page":"9207","DOI":"10.1158\/0008-5472.CAN-10-1743","article-title":"Human RecQL4 helicase plays critical roles in prostate carcinogenesis","volume":"70","author":"Su","year":"2010","journal-title":"Cancer Res"},{"key":"2026091804173441400_B22","doi-asserted-by":"publisher","first-page":"6102","DOI":"10.1128\/MCB.18.10.6102","article-title":"Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication","volume":"18","author":"Kamimura","year":"1998","journal-title":"Mol Cell Biol"},{"key":"2026091804173441400_B23","doi-asserted-by":"publisher","first-page":"791194","DOI":"10.3389\/fmolb.2021.791194","article-title":"Molecular mechanisms of the RECQ4 pathogenic mutations","volume":"8","author":"Xu","year":"2021","journal-title":"Front Mol Biosci"},{"key":"2026091804173441400_B24","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1016\/j.bbamcr.2011.01.001","article-title":"The N-terminal region of RECQL4 lacking the helicase domain is both essential and sufficient for the viability of vertebrate cells. Role of the N-terminal region of RECQL4 in cells","volume":"1813","author":"Abe","year":"2011","journal-title":"Biochim Biophys Acta"},{"key":"2026091804173441400_B25","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/j.celrep.2013.12.037","article-title":"Hrq1, a homolog of the human RecQ4 helicase, acts catalytically and structurally to promote genome integrity","volume":"6","author":"Bochman","year":"2014","journal-title":"Cell Rep"},{"key":"2026091804173441400_B26","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1016\/j.dnarep.2005.09.005","article-title":"Biochemical characterization of the RECQ4 protein, mutated in Rothmund-Thomson syndrome","volume":"5","author":"Macris","year":"2006","journal-title":"DNA Repair (Amst)"},{"key":"2026091804173441400_B27","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/j.compbiolchem.2008.07.005","article-title":"In silico analyses of a new group of fungal and plant RecQ4-homologous proteins","volume":"32","author":"Barea","year":"2008","journal-title":"Comput Biol Chem"},{"key":"2026091804173441400_B28","doi-asserted-by":"publisher","first-page":"1116","DOI":"10.1016\/j.bbrc.2017.04.003","article-title":"Saccharomyces cerevisiae Hrq1 helicase activity is affected by the sequence but not the length of single-stranded DNA","volume":"486","author":"Rogers","year":"2017","journal-title":"Biochem Biophys Res Commun"},{"key":"2026091804173441400_B29","doi-asserted-by":"publisher","first-page":"8945","DOI":"10.1074\/jbc.RA120.013626","article-title":"The yeast Hrq1 helicase stimulates Pso2 translesion nuclease activity and thereby promotes DNA interstrand crosslink repair","volume":"295","author":"Rogers","year":"2020","journal-title":"J Biol Chem"},{"key":"2026091804173441400_B30","doi-asserted-by":"publisher","first-page":"5217","DOI":"10.1093\/nar\/gkx151","article-title":"Yeast Hrq1 shares structural and functional homology with the disease-linked human RecQ4 helicase","volume":"45","author":"Rogers","year":"2017","journal-title":"Nucleic Acids Res"},{"key":"2026091804173441400_B31","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1016\/j.ymeth.2022.04.014","article-title":"Genetic and biochemical interactions of yeast DNA helicases","volume":"204","author":"Nickens","year":"2022","journal-title":"Methods"},{"key":"2026091804173441400_B32","doi-asserted-by":"publisher","first-page":"14481","DOI":"10.1074\/jbc.RA118.004092","article-title":"The Saccharomyces cerevisiae Hrq1 and Pif1 DNA helicases synergistically modulate telomerase activity in vitro","volume":"293","author":"Nickens","year":"2018","journal-title":"J Biol Chem"},{"key":"2026091804173441400_B33","doi-asserted-by":"publisher","first-page":"411","DOI":"10.3390\/genes10060411","article-title":"The biochemical activities of the Saccharomyces cerevisiae Pif1 helicase are regulated by its N-terminal domain","volume":"10","author":"Nickens","year":"2019","journal-title":"Genes (Basel)"},{"key":"2026091804173441400_B34","doi-asserted-by":"publisher","first-page":"4347","DOI":"10.1534\/g3.120.401864","article-title":"The genetic and physical interactomes of the Saccharomyces cerevisiae Hrq1 helicase","volume":"10","author":"Rogers","year":"2020","journal-title":"G3 (Bethesda)"},{"key":"2026091804173441400_B35","doi-asserted-by":"publisher","first-page":"4359","DOI":"10.1534\/g3.120.401709","article-title":"Comprehensive synthetic genetic array analysis of alleles that interact with mutation of the Saccharomyces cerevisiae RecQ helicases Hrq1 and Sgs1","volume":"10","author":"Sanders","year":"2020","journal-title":"G3 (Bethesda)"},{"key":"2026091804173441400_B36","doi-asserted-by":"publisher","first-page":"jkaf222","DOI":"10.1093\/g3journal\/jkaf222","article-title":"A screen for synthetic genetic interactions with the Saccharomyces cerevisiae hrq1DeltaN allele","volume":"15","author":"Shumaker","year":"2025","journal-title":"G3 (Bethesda)"},{"key":"2026091804173441400_B37","doi-asserted-by":"publisher","first-page":"e1010122","DOI":"10.1371\/journal.pgen.1010122","article-title":"Hrq1\/RECQL4 regulation is critical for preventing aberrant recombination during DNA intrastrand crosslink repair and is upregulated in breast cancer","volume":"18","author":"Luong","year":"2022","journal-title":"PLoS Genet"},{"key":"2026091804173441400_B38","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1007\/s00439-008-0518-4","article-title":"Sensitivity of RECQL4-deficient fibroblasts from Rothmund-Thomson syndrome patients to genotoxic agents","volume":"123","author":"Jin","year":"2008","journal-title":"Hum Genet"},{"key":"2026091804173441400_B39","doi-asserted-by":"publisher","first-page":"3057","DOI":"10.1158\/0008-5472.CAN-15-2361","article-title":"Human helicase RECQL4 drives cisplatin resistance in gastric cancer by activating an AKT-YB1-MDR1 signaling pathway","volume":"76","author":"Mo","year":"2016","journal-title":"Cancer Res"},{"key":"2026091804173441400_B40","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.pbiomolbio.2020.08.005","article-title":"Fanconi anemia-independent DNA inter-strand crosslink repair in eukaryotes","volume":"158","author":"Rogers","year":"2020","journal-title":"Prog Biophys Mol Biol"},{"key":"2026091804173441400_B41","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1038\/329219a0","article-title":"Functional inactivation of genes by dominant negative mutations","volume":"329","author":"Herskowitz","year":"1987","journal-title":"Nature"},{"key":"2026091804173441400_B42","doi-asserted-by":"publisher","first-page":"3843","DOI":"10.1105\/tpc.107.055053","article-title":"Exploring the molecular etiology of dominant-negative mutations","volume":"19","author":"Veitia","year":"2007","journal-title":"Plant Cell"},{"key":"2026091804173441400_B43","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/0092-8674(86)90483-6","article-title":"Normal stoichiometry of histone dimer sets is necessary for high fidelity of mitotic chromosome transmission","volume":"44","author":"Meeks-Wagner","year":"1986","journal-title":"Cell"},{"key":"2026091804173441400_B44","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/S0070-2153(08)60496-2","article-title":"Use of dominant negative constructs to modulate gene expression","volume":"36","author":"Lagna","year":"1998","journal-title":"Curr Top Dev Biol"},{"key":"2026091804173441400_B45","doi-asserted-by":"publisher","first-page":"20185","DOI":"10.1074\/jbc.M112132200","article-title":"ATP hydrolysis by mammalian RAD51 has a key role during homology-directed DNA repair","volume":"277","author":"Stark","year":"2002","journal-title":"J Biol Chem"},{"key":"2026091804173441400_B46","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1038\/nature01230","article-title":"Insights into DNA recombination from the structure of a RAD51\u2013BRCA2 complex","volume":"420","author":"Pellegrini","year":"2002","journal-title":"Nature"},{"key":"2026091804173441400_B47","doi-asserted-by":"publisher","first-page":"478","DOI":"10.1016\/j.molcel.2015.07.009","article-title":"A dominant mutation in human RAD51 reveals its function in DNA interstrand crosslink repair independent of homologous recombination","volume":"59","author":"Wang","year":"2015","journal-title":"Mol Cell"},{"key":"2026091804173441400_B48","doi-asserted-by":"publisher","first-page":"2651","DOI":"10.1093\/nar\/gkac073","article-title":"RAD51 protects against nonconservative DNA double-strand break repair through a nonenzymatic function","volume":"50","author":"So","year":"2022","journal-title":"Nucleic Acids Res"},{"key":"2026091804173441400_B49","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1006\/jmbi.1994.1003","article-title":"A dominant negative allele of the Escherichia coli uvrD gene encoding DNA helicase II. A biochemical and genetic characterization","volume":"235","author":"George","year":"1994","journal-title":"J Mol Biol"},{"key":"2026091804173441400_B50","doi-asserted-by":"publisher","first-page":"40853","DOI":"10.1074\/jbc.M208067200","article-title":"A dominant-negative mutant of human DNA helicase B blocks the onset of chromosomal DNA replication","volume":"277","author":"Taneja","year":"2002","journal-title":"J Biol Chem"},{"key":"2026091804173441400_B51","doi-asserted-by":"publisher","first-page":"4080","DOI":"10.4161\/cc.9.20.13667","article-title":"Helicase-inactivating mutations as a basis for dominant negative phenotypes","volume":"9","author":"Wu","year":"2010","journal-title":"Cell Cycle"},{"key":"2026091804173441400_B52","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1093\/genetics\/122.1.19","article-title":"A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae","volume":"122","author":"Sikorski","year":"1989","journal-title":"Genetics"},{"key":"2026091804173441400_B53","doi-asserted-by":"publisher","first-page":"953","DOI":"10.1002\/(SICI)1097-0061(199807)14:10%3c953::AID-YEA293%3e3.0.CO;2-U","article-title":"Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae","volume":"14","author":"Longtine","year":"1998","journal-title":"Yeast"},{"key":"2026091804173441400_B54","doi-asserted-by":"publisher","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","article-title":"Clustal W and Clustal X version 2.0","volume":"23","author":"Larkin","year":"2007","journal-title":"Bioinformatics"},{"key":"2026091804173441400_B55","doi-asserted-by":"crossref","first-page":"e1078","DOI":"10.1371\/journal.pone.0001078","article-title":"Optimized protein extraction for quantitative proteomics of yeasts","volume":"2","author":"von\u00a0der\u00a0Haar","year":"2007","journal-title":"PLoS One"},{"key":"2026091804173441400_B56","first-page":"29287","article-title":"Language models enable zero-shot prediction of the effects of mutations on protein function","volume":"34","author":"Meier","year":"2021","journal-title":"Adv Neur In"},{"key":"2026091804173441400_B57","doi-asserted-by":"publisher","first-page":"168967","DOI":"10.1016\/j.jmb.2025.168967","article-title":"AlphaFold protein structure database and 3D-beacons: new data and capabilities","volume":"437","author":"Fleming","year":"2025","journal-title":"J Mol Biol"},{"key":"2026091804173441400_B58","doi-asserted-by":"publisher","first-page":"W382","DOI":"10.1093\/nar\/gki387","article-title":"The FoldX web server: an online force field","volume":"33","author":"Schymkowitz","year":"2005","journal-title":"Nucleic Acids Res"},{"key":"2026091804173441400_B59","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1186\/s12859-015-0548-6","article-title":"MAESTRO\u2013multi agent stability prediction upon point mutations","volume":"16","author":"Laimer","year":"2015","journal-title":"BMC Bioinformatics"},{"key":"2026091804173441400_B60","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"Sievers","year":"2011","journal-title":"Mol Syst Biol"},{"key":"2026091804173441400_B61","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1016\/S0168-9525(00)02024-2","article-title":"EMBOSS: the European Molecular Biology Open Software Suite","volume":"16","author":"Rice","year":"2000","journal-title":"Trends Genet"},{"key":"2026091804173441400_B62","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1093\/nar\/gkaa1174","article-title":"A skipping rope translocation mechanism in a widespread family of DNA repair helicases","volume":"49","author":"Roske","year":"2021","journal-title":"Nucleic Acids Res"},{"key":"2026091804173441400_B63","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1002\/elps.1150090603","article-title":"Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250","volume":"9","author":"Neuhoff","year":"1988","journal-title":"Electrophoresis"},{"key":"2026091804173441400_B64","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.5012\/bkcs.2002.23.11.1511","article-title":"Highly sensitive and fast protein detection with coomassie brilliant blue in sodium dodecyl sulfate\u2013polyacrylamide gel electrophoresis","volume":"23","author":"Kang","year":"2002","journal-title":"B Korean Chem Soc"},{"key":"2026091804173441400_B65","doi-asserted-by":"publisher","first-page":"6317","DOI":"10.1093\/nar\/gkae265","article-title":"Cdc13 exhibits dynamic DNA strand exchange in the presence of telomeric DNA","volume":"52","author":"Nickens","year":"2024","journal-title":"Nucleic Acids Res"},{"key":"2026091804173441400_B66","doi-asserted-by":"publisher","first-page":"110496","DOI":"10.1016\/j.jbc.2025.110496","article-title":"Dimerization of Cdc13 is essential for dynamic DNA exchange on telomeric DNA","volume":"301","author":"Nickens","year":"2025","journal-title":"J Biol Chem"},{"key":"2026091804173441400_B67","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1007\/s00294-021-01190-3","article-title":"A deep dive into the RecQ interactome: something old and something new","volume":"67","author":"Simmons","year":"2021","journal-title":"Curr Genet"},{"key":"2026091804173441400_B68","doi-asserted-by":"publisher","first-page":"12760","DOI":"10.1038\/ncomms12760","article-title":"Excess of mutational jackpot events in expanding populations revealed by spatial Luria-Delbruck experiments","volume":"7","author":"Fusco","year":"2016","journal-title":"Nat Commun"},{"key":"2026091804173441400_B69","doi-asserted-by":"publisher","first-page":"3009","DOI":"10.1534\/g3.120.401396","article-title":"MutantHuntWGS: a pipeline for identifying Saccharomyces cerevisiae mutations","volume":"10","author":"Ellison","year":"2020","journal-title":"G3 (Bethesda)"},{"key":"2026091804173441400_B70","doi-asserted-by":"publisher","first-page":"e963429","DOI":"10.4161\/23723548.2014.963429","article-title":"Roles of DNA helicases in the maintenance of genome integrity","volume":"1","author":"Bochman","year":"2014","journal-title":"Mol Cell Oncol"},{"key":"2026091804173441400_B71","doi-asserted-by":"publisher","first-page":"338","DOI":"10.1111\/mmi.14158","article-title":"A bacterial DNA repair pathway specific to a natural antibiotic","volume":"111","author":"Burby","year":"2019","journal-title":"Mol Microbiol"},{"key":"2026091804173441400_B72","doi-asserted-by":"publisher","first-page":"D941","DOI":"10.1093\/nar\/gky1015","article-title":"COSMIC: the catalogue of somatic mutations in cancer","volume":"47","author":"Tate","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"2026091804173441400_B73","volume-title":"GeneReviews((R))","author":"Van\u00a0Maldergem","year":"1993"},{"key":"2026091804173441400_B74","doi-asserted-by":"publisher","first-page":"716","DOI":"10.1002\/ajmg.1320440604","article-title":"RAPADILINO syndrome","volume":"44","author":"Vargas","year":"1992","journal-title":"Am J Med Genet"},{"key":"2026091804173441400_B75","doi-asserted-by":"publisher","first-page":"7465","DOI":"10.1093\/nar\/gks417","article-title":"Mycobacterium smegmatis SftH exemplifies a distinctive clade of superfamily II DNA-dependent ATPases with 3\u2032 to 5\u2032 translocase and helicase activities","volume":"40","author":"Yakovleva","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"2026091804173441400_B76","doi-asserted-by":"publisher","first-page":"10171","DOI":"10.1073\/pnas.0900604106","article-title":"Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifs","volume":"106","author":"Kosugi","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"key":"2026091804173441400_B77","doi-asserted-by":"publisher","first-page":"16518","DOI":"10.1074\/jbc.M909511199","article-title":"The nuclease activity of the yeast DNA2 protein, which is related to the RecB-like nucleases, is essential in vivo","volume":"275","author":"Budd","year":"2000","journal-title":"J Biol Chem"},{"key":"2026091804173441400_B78","doi-asserted-by":"publisher","first-page":"E1992","DOI":"10.1073\/pnas.1300390110","article-title":"Nuclease activity of Saccharomyces cerevisiae Dna2 inhibits its potent DNA helicase activity","volume":"110","author":"Levikova","year":"2013","journal-title":"Proc Natl Acad Sci USA"},{"key":"2026091804173441400_B79","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1016\/j.molcel.2014.12.024","article-title":"TRF2 recruits RTEL1 to telomeres in S phase to promote t-loop unwinding","volume":"57","author":"Sarek","year":"2015","journal-title":"Mol Cell"},{"key":"2026091804173441400_B80","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1016\/j.cell.2012.03.030","article-title":"RTEL1 dismantles T loops and counteracts telomeric G4-DNA to maintain telomere integrity","volume":"149","author":"Vannier","year":"2012","journal-title":"Cell"},{"key":"2026091804173441400_B81","doi-asserted-by":"publisher","first-page":"1512","DOI":"10.1073\/pnas.0912986107","article-title":"LGP2 is a positive regulator of RIG-I- and MDA5-mediated antiviral responses","volume":"107","author":"Satoh","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"key":"2026091804173441400_B82","doi-asserted-by":"publisher","first-page":"938","DOI":"10.1074\/jbc.M112.424416","article-title":"ATP hydrolysis enhances RNA recognition and antiviral signal transduction by the innate immune sensor, laboratory of genetics and physiology 2 (LGP2)","volume":"288","author":"Bruns","year":"2013","journal-title":"J Biol Chem"},{"key":"2026091804173441400_B83","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1016\/j.molcel.2016.04.021","article-title":"Structural analysis of dsRNA binding to anti-viral pattern recognition receptors LGP2 and MDA5","volume":"62","author":"Uchikawa","year":"2016","journal-title":"Mol Cell"},{"key":"2026091804173441400_B84","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/j.dnarep.2010.01.006","article-title":"Rothmund-Thomson syndrome helicase, RECQ4: on the crossroad between DNA replication and repair","volume":"9","author":"Liu","year":"2010","journal-title":"DNA Repair (Amst)"},{"key":"2026091804173441400_B85","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1038\/emboj.2009.13","article-title":"Dual DNA unwinding activities of the Rothmund-Thomson syndrome protein, RECQ4","volume":"28","author":"Xu","year":"2009","journal-title":"Embo J"},{"key":"2026091804173441400_B86","doi-asserted-by":"publisher","first-page":"e69600","DOI":"10.1371\/journal.pone.0069600","article-title":"RecQL4 helicase amplification is involved in human breast tumorigenesis","volume":"8","author":"Fang","year":"2013","journal-title":"PLoS One"},{"key":"2026091804173441400_B87","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/j.cancergen.2025.02.013","article-title":"RECQL4-related Rothmund-Thomson syndrome: a case series and literature review","volume":"292\u2013293","author":"Ho","year":"2025","journal-title":"Cancer Genet"},{"key":"2026091804173441400_B88","first-page":"4248","article-title":"Upregulation of RECQL4 expression predicts poor prognosis in hepatocellular carcinoma","volume":"15","author":"Li","year":"2018","journal-title":"Oncol Lett"},{"key":"2026091804173441400_B89","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.canlet.2017.10.021","article-title":"Human RecQL4 helicase plays multifaceted roles in the genomic stability of normal and cancer cells","volume":"413","author":"Mo","year":"2018","journal-title":"Cancer Lett"},{"key":"2026091804173441400_B90","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1038\/ejhg.2008.154","article-title":"The mutation spectrum in RECQL4 diseases","volume":"17","author":"Siitonen","year":"2009","journal-title":"Eur J Hum Genet"}],"container-title":["Nucleic Acids Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/nar\/article-pdf\/54\/17\/gkag903\/71219728\/gkag903.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/nar\/article-pdf\/54\/17\/gkag903\/71219728\/gkag903.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T08:17:43Z","timestamp":1789719463000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/nar\/article\/doi\/10.1093\/nar\/gkag903\/8816729"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,7]]},"references-count":90,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2026,9,7]]}},"URL":"https:\/\/doi.org\/10.1093\/nar\/gkag903","relation":{},"ISSN":["0305-1048","1362-4962"],"issn-type":[{"value":"0305-1048","type":"print"},{"value":"1362-4962","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,9,23]]},"published":{"date-parts":[[2026,9,7]]},"article-number":"gkag903"}}