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The resulting protein-inclusive datasets are increasingly used to characterize cellular states, construct multimodal cell atlases, and decode disease mechanisms. However, such data are rarely included in existing databases. In this study, SingPro was updated from a single-cell proteomics database into a proteomics-centric knowledge base for single-cell multimodal profiling. Distinct from existing resources, the updated SingPro (a) systematically curates data and experimental details from 814 protein-inclusive multimodal studies encompassing ~81.5 million cells across 29 tissues, 44 diseases, and multiple species; (b) covers 5 omics modalities through 12 experimental technologies that simultaneously profile proteins with messenger RNA, chromatin accessibility, or other measurements in the same cell; and (c) provides cross-modality visualization for joint analysis. SingPro is freely accessible without requiring login or registration at: https:\/\/idrblab.org\/singpro\/.<\/jats:p>","DOI":"10.1093\/nar\/gkag933","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T19:16:57Z","timestamp":1789672617000},"source":"Crossref","is-referenced-by-count":0,"title":["SingPro 2.0: a proteomics-centric knowledge base for single-cell multimodal profiling"],"prefix":"10.1093","author":[{"given":"Yuan","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Pharmacy, Innovation Institute for Artificial Intelligence in Medicine of Zhejiang University, National Key Laboratory of Advanced Drug Delivery and Release Systems, Zhejiang University , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiannan","family":"Deng","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Innovation Institute for Artificial Intelligence in Medicine of Zhejiang University, 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Innovation Institute for Artificial Intelligence in Medicine of Zhejiang University, National Key Laboratory of Advanced Drug Delivery and Release Systems, Zhejiang University , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuna","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xichen","family":"Lian","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, The Second Affiliated Hospital, Zhejiang University School of Medicine , Hangzhou 310009 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7619-2975","authenticated-orcid":false,"given":"Minjie","family":"Mou","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Innovation Institute for Artificial Intelligence in Medicine of Zhejiang University, National Key Laboratory of Advanced Drug Delivery and Release Systems, Zhejiang University , Hangzhou 310058 ,","place":["China"]},{"name":"Department of Pharmacy, The Second Affiliated Hospital, Zhejiang University School of Medicine , Hangzhou 310009 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9607-7026","authenticated-orcid":false,"given":"Qingxia","family":"Yang","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases, Women\u2019s Hospital, Zhejiang University School of Medicine , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1381-9571","authenticated-orcid":false,"given":"Huaicheng","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Innovation Institute for Artificial Intelligence in Medicine of Zhejiang University, National Key Laboratory of Advanced Drug Delivery and Release Systems, Zhejiang University , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8069-0053","authenticated-orcid":false,"given":"Feng","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Innovation Institute for Artificial Intelligence in Medicine of Zhejiang University, National Key Laboratory of Advanced Drug Delivery and Release Systems, Zhejiang University , Hangzhou 310058 ,","place":["China"]},{"name":"Department of Pharmacy, The Second Affiliated Hospital, Zhejiang 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