{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T09:41:58Z","timestamp":1792575718841,"version":"4.5.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001677","name":"Inserm","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ecole Polytechnique. Y.C."},{"name":"Chinese Scholarship Council","award":["202308310081"],"award-info":[{"award-number":["202308310081"]}]},{"name":"Fondation de l\u2019Ecole Polytechnique"},{"name":"Agence de l\u2019Innovation de D\u00e9fense"},{"name":"Centre Interdisciplinaire d\u2019Etudes pour la D\u00e9fense et la S\u00e9curit\u00e9"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>G-quadruplexes (G4s) are non-canonical nucleic acid structures with critical roles in gene regulation, genomic stability, and disease, making them prime targets for therapeutic and biotechnological applications. However, the absence of a curated, centralized, experimentally validated repository of short (mostly synthetic) G4-forming sequences, paired with appropriate single-stranded or hairpin controls, has limited reproducibility and hindered progress in the field. Here, we introduce G4All, a comprehensive, curated database of G4-forming short DNA and RNA sequences, complemented by rigorously selected non-G4 controls, all studied under roughly similar experimental conditions (generally around 100 mM potassium and near neutral pH). G4All consolidates sequences validated by diverse experimental methods, including circular dichroism, NMR, UV spectroscopy, with annotations for topology, thermal stability, and experimental conditions. By providing both positive and negative datasets, G4All enables rigorous comparative analyses, assay benchmarking, and the development of predictive models. The database supports a broad range of applications, from fundamental studies of G4 biophysics to the rational design of aptamers, as well as benchmarking prediction algorithms. Future developments will expand G4All to include user-submitted datasets and more RNA sequences. Freely accessible at\u00a0https:\/\/g4-all.streamlit.app\/, G4All offers a searchable interface and downloadable datasets, establishing a community-driven hub to accelerate discovery and standardization in G4 research.<\/jats:p>","DOI":"10.1093\/nar\/gkag981","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T08:59:36Z","timestamp":1792573176000},"source":"Crossref","is-referenced-by-count":0,"title":["G4All: a database of experimentally confirmed G-quadruplex-forming sequences"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4165-2046","authenticated-orcid":false,"given":"Parth","family":"Rajput","sequence":"first","affiliation":[{"name":"Laboratoire d\u2019Optique et Biosciences, \u00c9cole Polytechnique, CNRS, Inserm, Institut Polytechnique de Paris , Palaiseau 91120 ,","place":["France"]},{"name":"Department of Biochemical Engineering and Biotechnology,\u00a0Indian Institute of Technology Delhi , Hauz Khas,\u00a0New Delhi, 110016 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Cucchiarini","sequence":"additional","affiliation":[{"name":"Laboratoire d\u2019Optique et Biosciences, \u00c9cole Polytechnique, CNRS, Inserm, Institut Polytechnique de Paris , Palaiseau 91120 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitrii","family":"Trubetskoy","sequence":"additional","affiliation":[{"name":"Laboratoire d\u2019Optique et Biosciences, \u00c9cole Polytechnique, CNRS, Inserm, Institut Polytechnique de Paris , Palaiseau 91120 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9536-9338","authenticated-orcid":false,"given":"Giacomo","family":"Ferrari","sequence":"additional","affiliation":[{"name":"Laboratoire d\u2019Optique et Biosciences, \u00c9cole Polytechnique, CNRS, Inserm, Institut Polytechnique de Paris , Palaiseau 91120 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,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3043-8401","authenticated-orcid":false,"given":"Jean-Louis","family":"Mergny","sequence":"additional","affiliation":[{"name":"Laboratoire d\u2019Optique et Biosciences, \u00c9cole Polytechnique, CNRS, Inserm, Institut Polytechnique de Paris , Palaiseau 91120 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,21]]},"reference":[{"key":"2026102104593397000_B1","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.trechm.2019.07.002","article-title":"The structure and function of DNA G-quadruplexes","volume":"2","author":"Spiegel","year":"2020","journal-title":"Trends Chem"},{"key":"2026102104593397000_B2","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1038\/s41580-020-0236-x","article-title":"The regulation and functions of DNA and RNA 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