{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T20:23:43Z","timestamp":1791059023296,"version":"4.1.0"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2016,10,4]],"date-time":"2016-10-04T00:00:00Z","timestamp":1475539200000},"content-version":"vor","delay-in-days":2173,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/2.5"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,3]]},"DOI":"10.1093\/nar\/gkq931","type":"journal-article","created":{"date-parts":[[2010,10,24]],"date-time":"2010-10-24T00:24:48Z","timestamp":1287879888000},"page":"1526-1537","source":"Crossref","is-referenced-by-count":133,"title":["The Bowen\u2013Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of \u03a81191 in yeast 18S rRNA"],"prefix":"10.1093","volume":"39","author":[{"given":"Britta","family":"Meyer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan Philip","family":"Wurm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"K\u00f6tter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias S.","family":"Leisegang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeska","family":"Schilling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Buchhaupt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Held","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Bahr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Karas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Heckel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus T.","family":"Bohnsack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"W\u00f6hnert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl-Dieter","family":"Entian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2010,10,23]]},"reference":[{"key":"key\n\t\t\t\t20170510094423_B1","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1128\/EC.3.6.1619-1626.2004","article-title":"The small-subunit processome is a ribosome assembly intermediate","volume":"3","author":"Bernstein","year":"2004","journal-title":"Eukaryot. Cell"},{"key":"key\n\t\t\t\t20170510094423_B2","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S1097-2765(02)00579-8","article-title":"90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors","volume":"10","author":"Grandi","year":"2002","journal-title":"Mol. Cell"},{"key":"key\n\t\t\t\t20170510094423_B3","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/S0968-0004(99)01460-7","article-title":"The economics of ribosome biosynthesis in yeast","volume":"24","author":"Warner","year":"1999","journal-title":"Trends Biochem. Sci."},{"key":"key\n\t\t\t\t20170510094423_B4","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0378-1119(03)00629-2","article-title":"Ribosome assembly in eukaryotes","volume":"313","author":"Fromont-Racine","year":"2003","journal-title":"Gene"},{"key":"key\n\t\t\t\t20170510094423_B5","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0962-8924(03)00054-0","article-title":"Pre-ribosomes on the road from the nucleolus to the cytoplasm","volume":"13","author":"Tschochner","year":"2003","journal-title":"Trends Cell Biol."},{"key":"key\n\t\t\t\t20170510094423_B6","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1146\/annurev.genet.33.1.261","article-title":"Ribosome synthesis in Saccharomyces cerevisiae","volume":"33","author":"Venema","year":"1999","journal-title":"Annu. Rev. Genet."},{"key":"key\n\t\t\t\t20170510094423_B7","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1016\/S0092-8674(00)81308-2","article-title":"Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs","volume":"85","author":"Kiss-Laszlo","year":"1996","journal-title":"Cell"},{"key":"key\n\t\t\t\t20170510094423_B8","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/S0092-8674(00)80263-9","article-title":"Site-specific pseudouridine formation in preribosomal RNA is guided by small nucleolar RNAs","volume":"89","author":"Ganot","year":"1997","journal-title":"Cell"},{"key":"key\n\t\t\t\t20170510094423_B9","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1006\/jmbi.1994.1525","article-title":"The DIM1 gene responsible for the conserved m6(2)Am6(2)A dimethylation in the 3\u2032-terminal loop of 18\u2009S rRNA is essential in yeast","volume":"241","author":"Lafontaine","year":"1994","journal-title":"J. Mol. Biol."},{"key":"key\n\t\t\t\t20170510094423_B10","doi-asserted-by":"crossref","first-page":"3151","DOI":"10.1128\/MCB.01674-07","article-title":"Bud23 methylates G1575 of 18S rRNA and is required for efficient nuclear export of pre-40S subunits","volume":"28","author":"White","year":"2008","journal-title":"Mol. Cell. Biol."},{"key":"key\n\t\t\t\t20170510094423_B11","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/0014-5793(75)80341-3","article-title":"Presence of a hypermodified nucleotide in HeLa cell 18\u2009S and Saccharomyces carlsbergensis 17\u2009S ribosomal RNAs","volume":"59","author":"Maden","year":"1975","journal-title":"FEBS Lett."},{"key":"key\n\t\t\t\t20170510094423_B12","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.1261\/rna.1724409","article-title":"Loss of rRNA modifications in the decoding center of the ribosome impairs translation and strongly delays pre-rRNA processing","volume":"15","author":"Liang","year":"2009","journal-title":"RNA"},{"key":"key\n\t\t\t\t20170510094423_B13","doi-asserted-by":"crossref","first-page":"2548","DOI":"10.1093\/nar\/23.13.2548","article-title":"Characterization of three new snRNAs from Saccharomyces cerevisiae: snR34, snR35 and snR36","volume":"23","author":"Samarsky","year":"1995","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20170510094423_B14","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1042\/bj1690071","article-title":"Biosynthesis of a hypermodified nucleotide in Saccharomyces carlsbergensis 17S and HeLa-cell 18S ribosomal ribonucleic acid","volume":"169","author":"Brand","year":"1978","journal-title":"Biochem. J."},{"key":"key\n\t\t\t\t20170510094423_B15","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/0022-2836(68)90298-2","article-title":"Incorporation of 14C from [2-14C]methionine into 18\u2009S but not 28\u2009S RNA of Chinese hamster cells","volume":"33","author":"Enger","year":"1968","journal-title":"J. Mol. Biol."},{"key":"key\n\t\t\t\t20170510094423_B16","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0005-2787(74)90009-4","article-title":"The isolation from ribonucleic acid of substituted uridines containing alpha-aminobutyrate moieties derived from methionine","volume":"349","author":"Saponara","year":"1974","journal-title":"Biochim. Biophys. Acta"},{"key":"key\n\t\t\t\t20170510094423_B17","doi-asserted-by":"crossref","first-page":"24484","DOI":"10.1074\/jbc.M002153200","article-title":"Identities and phylogenetic comparisons of posttranscriptional modifications in 16\u2009S ribosomal RNA from Haloferax volcanii","volume":"275","author":"Kowalak","year":"2000","journal-title":"J. Biol. Chem."},{"key":"key\n\t\t\t\t20170510094423_B18","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1038\/nrg2142","article-title":"Translation matters: protein synthesis defects in inherited disease","volume":"8","author":"Scheper","year":"2007","journal-title":"Nat. Rev. Genet."},{"key":"key\n\t\t\t\t20170510094423_B19","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1111\/j.1365-2141.2008.07095.x","article-title":"Ribosomal dysfunction and inherited marrow failure","volume":"141","author":"Ganapathi","year":"2008","journal-title":"Br. J. Haematol."},{"key":"key\n\t\t\t\t20170510094423_B20","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1111\/j.1365-2141.2009.07692.x","article-title":"Bone marrow cells from patients with Shwachman\u2013Diamond syndrome abnormally express genes involved in ribosome biogenesis and RNA processing","volume":"145","author":"Rujkijyanont","year":"2009","journal-title":"Br. J. Haematol."},{"key":"key\n\t\t\t\t20170510094423_B21","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1038\/5951","article-title":"The gene encoding ribosomal protein S19 is mutated in Diamond\u2013Blackfan anaemia","volume":"21","author":"Draptchinskaia","year":"1999","journal-title":"Nat. Genet."},{"key":"key\n\t\t\t\t20170510094423_B22","doi-asserted-by":"crossref","first-page":"38177","DOI":"10.1074\/jbc.M506916200","article-title":"Specific role for yeast homologs of the Diamond\u2013Blackfan anemia-associated Rps19 protein in ribosome synthesis","volume":"280","author":"Leger-Silvestre","year":"2005","journal-title":"J. Biol. Chem."},{"key":"key\n\t\t\t\t20170510094423_B23","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1182\/blood-2006-07-038372","article-title":"Impaired ribosome biogenesis in Diamond\u2013Blackfan anemia","volume":"109","author":"Choesmel","year":"2007","journal-title":"Blood"},{"key":"key\n\t\t\t\t20170510094423_B24","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.bcmd.2007.02.001","article-title":"Cells depleted for RPS19, a protein associated with Diamond Blackfan anemia, show defects in 18S ribosomal RNA synthesis and small ribosomal subunit production","volume":"39","author":"Idol","year":"2007","journal-title":"Blood Cells Mol. Dis."},{"key":"key\n\t\t\t\t20170510094423_B25","doi-asserted-by":"crossref","first-page":"3644","DOI":"10.1091\/mbc.12.11.3644","article-title":"Novel stress-responsive genes EMG1 and NOP14 encode conserved, interacting proteins required for 40S ribosome biogenesis","volume":"12","author":"Liu","year":"2001","journal-title":"Mol. Biol. Cell"},{"key":"key\n\t\t\t\t20170510094423_B26","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1007\/s00294-001-0269-4","article-title":"Nep1p (Emg1p), a novel protein conserved in eukaryotes and archaea, is involved in ribosome biogenesis","volume":"40","author":"Eschrich","year":"2002","journal-title":"Curr. Genet."},{"key":"key\n\t\t\t\t20170510094423_B27","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/j.ajhg.2009.04.017","article-title":"Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen\u2013Conradi syndrome","volume":"84","author":"Armistead","year":"2009","journal-title":"Am. J. Hum. Genet."},{"key":"key\n\t\t\t\t20170510094423_B28","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1002\/ajmg.a.20059","article-title":"Bowen\u2013Conradi syndrome: a clinical and genetic study","volume":"120A","author":"Lowry","year":"2003","journal-title":"Am. J. Med. Genet. A"},{"key":"key\n\t\t\t\t20170510094423_B29","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1093\/nar\/gkm1074","article-title":"The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha\/beta knot fold methyltransferases","volume":"36","author":"Leulliot","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20170510094423_B30","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1093\/nar\/gkm1172","article-title":"The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site","volume":"36","author":"Taylor","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20170510094423_B31","first-page":"71","article-title":"SPOUT: a class of methyltransferases that includes spoU and trmD RNA methylase superfamilies, and novel superfamilies of predicted prokaryotic RNA methylases","volume":"4","author":"Anantharaman","year":"2002","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"key\n\t\t\t\t20170510094423_B32","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/1471-2105-8-73","article-title":"Structural and evolutionary bioinformatics of the SPOUT superfamily of methyltransferases","volume":"8","author":"Tkaczuk","year":"2007","journal-title":"BMC Bioinformatics"},{"key":"key\n\t\t\t\t20170510094423_B33","doi-asserted-by":"crossref","first-page":"2223","DOI":"10.1261\/rna.1186608","article-title":"Identification of pseudouridine methyltransferase in Escherichia coli","volume":"14","author":"Ero","year":"2008","journal-title":"RNA"},{"key":"key\n\t\t\t\t20170510094423_B34","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1093\/nar\/gkp1189","article-title":"The ribosome assembly factor Nep1 responsible for Bowen\u2013Conradi syndrome is a pseudouridine-N1-specific methyltransferase","volume":"38","author":"Wurm","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20170510094423_B35","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1006\/abbi.1999.1498","article-title":"Saccharomyces cerevisiae expresses two genes encoding isozymes of methylenetetrahydrofolate reductase","volume":"372","author":"Raymond","year":"1999","journal-title":"Arch. Biochem. Biophys."},{"key":"key\n\t\t\t\t20170510094423_B36","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0021-9673(00)94152-9","article-title":"Ribonucleoside analysis by reversed-phase high-performance liquid chromatography","volume":"471","author":"Gehrke","year":"1989","journal-title":"J. Chromatogr."},{"key":"key\n\t\t\t\t20170510094423_B37","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s00438-006-0132-x","article-title":"Genetic evidence for 18S rRNA binding and an Rps19p assembly function of yeast nucleolar protein Nep1p","volume":"276","author":"Buchhaupt","year":"2006","journal-title":"Mol. Genet. Genomics"},{"key":"key\n\t\t\t\t20170510094423_B38","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1111\/j.1432-1033.1985.tb08704.x","article-title":"Specific fragmentation of tRNA and rRNA at a 7-methylguanine residue in the presence of methylated carrier RNA","volume":"146","author":"Zueva","year":"1985","journal-title":"Eur. J. Biochem."},{"key":"key\n\t\t\t\t20170510094423_B39","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1111\/j.1567-1364.2007.00230.x","article-title":"Mutations in the nucleolar proteins Tma23 and Nop6 suppress the malfunction of the Nep1 protein","volume":"7","author":"Buchhaupt","year":"2007","journal-title":"FEMS Yeast Res."},{"key":"key\n\t\t\t\t20170510094423_B40","first-page":"629","article-title":"","volume-title":"Methods in Microbiology","author":"Entian","year":"2007"},{"key":"key\n\t\t\t\t20170510094423_B41","doi-asserted-by":"crossref","first-page":"e120","DOI":"10.1093\/nar\/gkp578","article-title":"A fast and efficient translational control system for conditional expression of yeast genes","volume":"37","author":"K\u00f6tter","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20170510094423_B42","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/415141a","article-title":"Functional organization of the yeast proteome by systematic analysis of protein complexes","volume":"415","author":"Gavin","year":"2002","journal-title":"Nature"},{"key":"key\n\t\t\t\t20170510094423_B43","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1074\/mcp.M600381-MCP200","article-title":"Toward a comprehensive atlas of the physical interactome of Saccharomyces cerevisiae","volume":"6","author":"Collins","year":"2007","journal-title":"Mol. Cell Proteomics"}],"container-title":["Nucleic Acids Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/nar\/article-pdf\/39\/4\/1526\/16781650\/gkq931.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,4,2]],"date-time":"2018-04-02T14:41:52Z","timestamp":1522680112000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/nar\/article-lookup\/doi\/10.1093\/nar\/gkq931"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10,23]]},"references-count":43,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2010,10,23]]},"published-print":{"date-parts":[[2011,3]]}},"URL":"https:\/\/doi.org\/10.1093\/nar\/gkq931","relation":{},"ISSN":["1362-4962","0305-1048"],"issn-type":[{"value":"1362-4962","type":"electronic"},{"value":"0305-1048","type":"print"}],"subject":[],"published":{"date-parts":[[2010,10,23]]}}}