{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T07:29:06Z","timestamp":1792049346032,"version":"4.4.0"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2016,11,10]],"date-time":"2016-11-10T00:00:00Z","timestamp":1478736000000},"content-version":"vor","delay-in-days":1024,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The \u03c3 subunit of bacterial RNA polymerase (RNAP) has been implicated in all steps of transcription initiation, including promoter recognition and opening, priming of RNA synthesis, abortive initiation and promoter escape. The post-promoter-recognition \u03c3 functions were proposed to depend on its conserved region \u03c33.2 that directly contacts promoter DNA immediately upstream of the RNAP active centre and occupies the RNA exit path. Analysis of the transcription effects of substitutions and deletions in this region in Escherichia coli \u03c370 subunit, performed in this work, suggests that (i) individual residues in the \u03c33.2 finger collectively contribute to RNA priming by RNAP, likely by the positioning of the template DNA strand in the active centre, but are not critical to promoter escape; (ii) the physical presence of \u03c33.2 in the RNA exit channel is important for promoter escape; (iii) \u03c33.2 promotes \u03c3 dissociation during initiation and suppresses \u03c3-dependent promoter-proximal pausing; (iv) \u03c33.2 contributes to allosteric inhibition of the initiating NTP binding by rifamycins. Thus, region \u03c33.2 performs distinct functions in transcription initiation and its inhibition by antibiotics. The B-reader element of eukaryotic factor TFIIB likely plays similar roles in RNAPII transcription, revealing common principles in transcription initiation in various domains of life.<\/jats:p>","DOI":"10.1093\/nar\/gkt1384","type":"journal-article","created":{"date-parts":[[2014,1,23]],"date-time":"2014-01-23T01:35:55Z","timestamp":1390440955000},"page":"4494-4504","source":"Crossref","is-referenced-by-count":64,"title":["Distinct functions of the RNA polymerase \u03c3 subunit region 3.2 in RNA priming and promoter escape"],"prefix":"10.1093","volume":"42","author":[{"given":"Danil","family":"Pupov","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Genetics of Microorganisms, Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Kuzin","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics of Microorganisms, Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Bass","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics of Microorganisms, Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey","family":"Kulbachinskiy","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics of Microorganisms, Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,1,21]]},"reference":[{"key":"2022072908344856600_gkt1384-B1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0959-440X(02)00005-2","article-title":"Bacterial RNA polymerases: the wholo story","volume":"13","author":"Murakami","year":"2003","journal-title":"Curr. 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