{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T01:42:29Z","timestamp":1778895749221,"version":"3.51.4"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"22","license":[{"start":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T00:00:00Z","timestamp":1478476800000},"content-version":"vor","delay-in-days":718,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,12,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Yeast mitochondria contain a minimalist threonyl-tRNA synthetase (ThrRS) composed only of the catalytic core and tRNA binding domain but lacking the entire editing domain. Besides the usual tRNAThr2, some budding yeasts, such as Saccharomyces cerevisiae, also contain a non-canonical tRNAThr1 with an enlarged 8-nucleotide anticodon loop, reprograming the usual leucine CUN codons to threonine. This raises interesting questions about the aminoacylation fidelity of such ThrRSs and the possible contribution of the two tRNAThrs during editing. Here, we found that, despite the absence of the editing domain, S. cerevisiae mitochondrial ThrRS (ScmtThrRS) harbors a tRNA-dependent pre-transfer editing activity. Remarkably, only the usual tRNAThr2 stimulated pre-transfer editing, thus, establishing the first example of a synthetase exhibiting tRNA-isoacceptor specificity during pre-transfer editing. We also showed that the failure of tRNAThr1 to stimulate tRNA-dependent pre-transfer editing was due to the lack of an editing domain. Using assays of the complementation of a ScmtThrRS gene knockout strain, we showed that the catalytic core and tRNA binding domain of ScmtThrRS co-evolved to recognize the unusual tRNAThr1. In combination, the results provide insights into the tRNA-dependent editing process and suggest that tRNA-dependent pre-transfer editing takes place in the aminoacylation catalytic core.<\/jats:p>","DOI":"10.1093\/nar\/gku1218","type":"journal-article","created":{"date-parts":[[2014,11,20]],"date-time":"2014-11-20T21:24:42Z","timestamp":1416518682000},"page":"13873-13886","source":"Crossref","is-referenced-by-count":21,"title":["A minimalist mitochondrial threonyl-tRNA synthetase exhibits tRNA-isoacceptor specificity during proofreading"],"prefix":"10.1093","volume":"42","author":[{"given":"Xiao-Long","family":"Zhou","sequence":"first","affiliation":[{"name":"1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-Rong","family":"Ruan","sequence":"additional","affiliation":[{"name":"1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Wang","sequence":"additional","affiliation":[{"name":"1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-Peng","family":"Fang","sequence":"additional","affiliation":[{"name":"1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Wang","sequence":"additional","affiliation":[{"name":"2School of Life Science and Technology, ShanghaiTech University, 319 Yue Yang Road, 200031 Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Chen","sequence":"additional","affiliation":[{"name":"1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ru-Juan","family":"Liu","sequence":"additional","affiliation":[{"name":"1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilbert","family":"Eriani","sequence":"additional","affiliation":[{"name":"3Architecture et R\u00e9activit\u00e9 de l'ARN, Universit\u00e9 de Strasbourg, UPR9002 CNRS, Institut de Biologie Mol\u00e9culaire et Cellulaire, 15 rue Ren\u00e9 Descartes, 67084 Strasbourg, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"En-Duo","family":"Wang","sequence":"additional","affiliation":[{"name":"1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, China"},{"name":"2School of Life Science and Technology, ShanghaiTech University, 319 Yue Yang Road, 200031 Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2014,11,20]]},"reference":[{"key":"2026042723511421700_B1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/nature05002","article-title":"Molecular biology: sticky end in protein synthesis","volume":"443","author":"Roy","year":"2006","journal-title":"Nature"},{"key":"2026042723511421700_B2","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1146\/annurev.biochem.69.1.617","article-title":"Aminoacyl-tRNA synthesis","volume":"69","author":"Ibba","year":"2000","journal-title":"Annu. 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China Life Sci."},{"key":"2026042723511421700_B6","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1146\/annurev.micro.091208.073210","article-title":"Aminoacyl-tRNA synthesis and translational quality control","volume":"63","author":"Ling","year":"2009","journal-title":"Annu. Rev. Microbiol."},{"key":"2026042723511421700_B7","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/nature05096","article-title":"Editing-defective tRNA synthetase causes protein misfolding and neurodegeneration","volume":"443","author":"Lee","year":"2006","journal-title":"Nature"},{"key":"2026042723511421700_B8","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1016\/j.chembiol.2006.08.011","article-title":"Global effects of mistranslation from an editing defect in mammalian cells","volume":"13","author":"Nangle","year":"2006","journal-title":"Chem. 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Biol. Chem."},{"key":"2026042723511421700_B18","doi-asserted-by":"crossref","first-page":"23799","DOI":"10.1074\/jbc.M110.133553","article-title":"Partitioning of tRNA-dependent editing between pre- and post-transfer pathways in class I aminoacyl-tRNA synthetases","volume":"285","author":"Dulic","year":"2010","journal-title":"J. Biol. Chem."},{"key":"2026042723511421700_B19","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1093\/nar\/gks982","article-title":"Translational fidelity maintenance preventing Ser mis-incorporation at Thr codon in protein from eukaryote","volume":"41","author":"Zhou","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"2026042723511421700_B20","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1007\/128_2013_457","article-title":"Pathogenic implications of human mitochondrial aminoacyl-tRNA synthetases","volume":"344","author":"Schwenzer","year":"2014","journal-title":"Top. Curr. 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