{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T09:12:49Z","timestamp":1790500369229,"version":"4.1.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2017,3,21]],"date-time":"2017-03-21T00:00:00Z","timestamp":1490054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,6,20]]},"DOI":"10.1093\/nar\/gkx195","type":"journal-article","created":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T17:01:52Z","timestamp":1489510912000},"page":"6717-6728","source":"Crossref","is-referenced-by-count":39,"title":["Critical 23S rRNA interactions for macrolide-dependent ribosome stalling on the ErmCL nascent peptide chain"],"prefix":"10.1093","volume":"45","author":[{"given":"Miriam","family":"Koch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Willi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ugo","family":"Prad\u00e8re","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Hall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert","family":"Polacek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,3,21]]},"reference":[{"key":"key\n\t\t\t\t20170620071201_B1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.4161\/rna.5.1.5922","article-title":"Ribosomal catalysis: the evolution of mechanistic concepts for peptide bond formation and peptidyl-tRNA hydrolysis","volume":"5","year":"2008","journal-title":"RNA Biol."},{"key":"key\n\t\t\t\t20170620071201_B2","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1080\/10409230500326334","article-title":"The ribosomal peptidyl transferase center: structure, function, evolution, inhibition","volume":"40","year":"2005","journal-title":"Crit. Rev. Biochem. Mol."},{"key":"key\n\t\t\t\t20170620071201_B3","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1126\/science.289.5481.905","article-title":"The complete atomic structure of the large ribosomal subunit at 2.4 A resolution","volume":"289","year":"2000","journal-title":"Science"},{"key":"key\n\t\t\t\t20170620071201_B4","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1126\/science.289.5481.920","article-title":"The structural basis of ribosome activity in peptide bond synthesis","volume":"289","year":"2000","journal-title":"Science"},{"key":"key\n\t\t\t\t20170620071201_B5","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1016\/S0092-8674(01)00546-3","article-title":"High resolution structure of the large ribosomal subunit from a mesophilic eubacterium","volume":"107","year":"2001","journal-title":"Cell"},{"key":"key\n\t\t\t\t20170620071201_B6","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1126\/science.1117230","article-title":"Structures of the bacterial ribosome at 3.5 A resolution","volume":"310","year":"2005","journal-title":"Science"},{"key":"key\n\t\t\t\t20170620071201_B7","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.1126\/science.1131127","article-title":"Structure of the 70S ribosome complexed with mRNA and tRNA","volume":"313","year":"2006","journal-title":"Science"},{"key":"key\n\t\t\t\t20170620071201_B8","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0896-6273(03)00506-3","article-title":"The birth of a channel","volume":"40","year":"2003","journal-title":"Neuron"},{"key":"key\n\t\t\t\t20170620071201_B9","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.sbi.2016.01.008","article-title":"Translation regulation via nascent polypeptide-mediated ribosome stalling","volume":"37","year":"2016","journal-title":"Curr. Opin. Struct. Biol."},{"key":"key\n\t\t\t\t20170620071201_B10","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.sbi.2011.01.007","article-title":"The ribosomal tunnel as a functional environment for nascent polypeptide folding and translational stalling","volume":"21","year":"2011","journal-title":"Curr. Opin. Struct. Biol."},{"key":"key\n\t\t\t\t20170620071201_B11","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1146\/annurev-biochem-080211-105026","article-title":"Arrest peptides: cis-acting modulators of translation","volume":"82","year":"2013","journal-title":"Annu. Rev. Biochem."},{"key":"key\n\t\t\t\t20170620071201_B12","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1111\/j.1365-2958.2008.06576.x","article-title":"Programmed drug-dependent ribosome stalling","volume":"71","year":"2009","journal-title":"Mol. Microbiol."},{"key":"key\n\t\t\t\t20170620071201_B13","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1007\/BF00331851","article-title":"Translational attenuation of ermC: a deletion analysis","volume":"186","year":"1982","journal-title":"Mol. Gen. Genet."},{"key":"key\n\t\t\t\t20170620071201_B14","first-page":"69","article-title":"ErmC leader peptide. Amino acid sequence critical for induction by translational attenuation","volume":"206","year":"1989","journal-title":"J. Mol. Biol."},{"key":"key\n\t\t\t\t20170620071201_B15","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1038\/nchembio.1998","article-title":"Nascent peptide assists the ribosome in recognizing chemically distinct small molecules","volume":"12","year":"2016","journal-title":"Nat. Chem. Biol."},{"key":"key\n\t\t\t\t20170620071201_B16","doi-asserted-by":"crossref","first-page":"3108","DOI":"10.1038\/emboj.2010.180","article-title":"The key function of a conserved and modified rRNA residue in the ribosomal response to the nascent peptide","volume":"29","year":"2010","journal-title":"EMBO J."},{"key":"key\n\t\t\t\t20170620071201_B17","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.molcel.2008.02.026","article-title":"Molecular mechanism of drug-dependent ribosome stalling","volume":"30","year":"2008","journal-title":"Mol. Cell"},{"key":"key\n\t\t\t\t20170620071201_B18","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.molcel.2014.09.014","article-title":"Drug sensing by the ribosome induces translational arrest via active site perturbation","volume":"56","year":"2014","journal-title":"Mol. Cell"},{"key":"key\n\t\t\t\t20170620071201_B19","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.jmb.2008.03.075","article-title":"Mutations outside the anisomycin-binding site can make ribosomes drug-resistant","volume":"379","year":"2008","journal-title":"J. Mol. Biol."},{"key":"key\n\t\t\t\t20170620071201_B20","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1038\/nprot.2011.306","article-title":"Generation of chemically engineered ribosomes for atomic mutagenesis studies on protein biosynthesis","volume":"6","year":"2011","journal-title":"Nat. Protoc."},{"key":"key\n\t\t\t\t20170620071201_B21","doi-asserted-by":"crossref","first-page":"1618","DOI":"10.1093\/nar\/gki308","article-title":"Chemical engineering of the peptidyl transferase center reveals an important role of the 2\u0384-hydroxyl group of A2451","volume":"33","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20170620071201_B22","doi-asserted-by":"crossref","first-page":"E2561","DOI":"10.1073\/pnas.1505231112","article-title":"Role of a ribosomal RNA phosphate oxygen during the EF-G-triggered GTP hydrolysis","volume":"112","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"key\n\t\t\t\t20170620071201_B23","doi-asserted-by":"crossref","first-page":"4453","DOI":"10.1021\/ja0588454","article-title":"Efficient ribosomal peptidyl transfer critically relies on the presence of the ribose 2\u0384-OH at A2451 of 23S rRNA","volume":"128","year":"2006","journal-title":"J. Am. Chem. Soc."},{"key":"key\n\t\t\t\t20170620071201_B24","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1006\/jmbi.1999.3030","article-title":"Effect of antibiotics on large ribosomal subunit assembly reveals possible function of 5S rRNA","volume":"291","year":"1999","journal-title":"J. Mol. Biol."},{"key":"key\n\t\t\t\t20170620071201_B25","doi-asserted-by":"crossref","first-page":"4844","DOI":"10.1093\/nar\/gkq213","article-title":"The role of the universally conserved A2450-C2063 base pair in the ribosomal peptidyl transferase center","volume":"38","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20170620071201_B26","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/978-1-61779-545-9_14","article-title":"Probing functions of the ribosomal peptidyl transferase center by nucleotide analog interference","volume":"848","year":"2012","journal-title":"Methods Mol. Biol."},{"key":"key\n\t\t\t\t20170620071201_B27","doi-asserted-by":"crossref","first-page":"12028","DOI":"10.1002\/anie.201304986","article-title":"Chemical synthesis of mono- and bis-labeled pre-microRNAs","volume":"52","year":"2013","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"key\n\t\t\t\t20170620071201_B28","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1089\/nat.2011.0329","article-title":"Properties of N(4)-methylated cytidines in miRNA mimics","volume":"22","year":"2012","journal-title":"Nucleic Acid Therap."},{"key":"key\n\t\t\t\t20170620071201_B29","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1021\/bc00030a005","article-title":"Synthesis of uridine phosphoramidite analogs: reagents for site-specific incorporation of photoreactive sites into RNA sequences","volume":"5","year":"1994","journal-title":"Bioconjug. Chem."},{"key":"key\n\t\t\t\t20170620071201_B30","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1080\/15476286.2015.1017218","article-title":"The integrity of the G2421-C2395 base pair in the ribosomal E-site is crucial for protein synthesis","volume":"12","year":"2015","journal-title":"RNA Biol."},{"key":"key\n\t\t\t\t20170620071201_B31","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.1017\/S1355838298980347","article-title":"Metal ion probing of rRNAs: evidence for evolutionarily conserved divalent cation binding pockets","volume":"4","year":"1998","journal-title":"RNA"},{"key":"key\n\t\t\t\t20170620071201_B32","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.1021\/bi9822473","article-title":"Reconstitution of functionally active Thermus aquaticus large ribosomal subunits with in vitro-transcribed rRNA","volume":"38","year":"1999","journal-title":"Biochemistry"},{"key":"key\n\t\t\t\t20170620071201_B33","doi-asserted-by":"crossref","first-page":"5130","DOI":"10.1093\/nar\/gkm539","article-title":"An intact ribose moiety at A2602 of 23S rRNA is key to trigger peptidyl-tRNA hydrolysis during translation termination","volume":"35","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20170620071201_B34","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S1097-2765(02)00825-0","article-title":"The critical role of the universally conserved A2602 of 23S ribosomal RNA in the release of the nascent peptide during translation termination","volume":"11","year":"2003","journal-title":"Mol. Cell"},{"key":"key\n\t\t\t\t20170620071201_B35","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/S0092-8674(04)00411-8","article-title":"The active site of the ribosome is composed of two layers of conserved nucleotides with distinct roles in peptide bond formation and peptide release","volume":"117","year":"2004","journal-title":"Cell"},{"key":"key\n\t\t\t\t20170620071201_B36","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.jmb.2009.01.037","article-title":"Statics of the ribosomal exit tunnel: implications for cotranslational peptide folding, elongation regulation, and antibiotics binding","volume":"387","year":"2009","journal-title":"J. Mol. Biol."},{"key":"key\n\t\t\t\t20170620071201_B37","doi-asserted-by":"crossref","first-page":"4961","DOI":"10.1128\/AAC.00860-10","article-title":"Binding and action of CEM-101, a new fluoroketolide antibiotic that inhibits protein synthesis","volume":"54","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"key\n\t\t\t\t20170620071201_B38","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1038\/nchembio.341","article-title":"Atomic mutagenesis reveals A2660 of 23S ribosomal RNA as key to EF-G GTPase activation","volume":"6","year":"2010","journal-title":"Nat. Chem. Biol."},{"key":"key\n\t\t\t\t20170620071201_B39","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1038\/nsmb.2871","article-title":"A proton wire to couple aminoacyl-tRNA accommodation and peptide-bond formation on the ribosome","volume":"21","year":"2014","journal-title":"Nat. Struct. Mol. Biol."},{"key":"key\n\t\t\t\t20170620071201_B40","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.chembiol.2008.03.014","article-title":"The role of 23S ribosomal RNA residue A2451 in peptide bond synthesis revealed by atomic mutagenesis","volume":"15","year":"2008","journal-title":"Chem. Biol."},{"key":"key\n\t\t\t\t20170620071201_B41","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1134\/S0006297915080106","article-title":"Molecular Dynamics Investigation of a Mechanism of Allosteric Signal Transmission in Ribosomes","volume":"80","year":"2015","journal-title":"Biochemistry (Mosc.)"},{"key":"key\n\t\t\t\t20170620071201_B42","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S1097-2765(02)00570-1","article-title":"The structures of four macrolide antibiotics bound to the large ribosomal subunit","volume":"10","year":"2002","journal-title":"Mol. Cell"},{"key":"key\n\t\t\t\t20170620071201_B43","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.molcel.2005.09.006","article-title":"Structural insights into the roles of water and the 2\u0384 hydroxyl of the P site tRNA in the peptidyl transferase reaction","volume":"20","year":"2005","journal-title":"Mol. Cell"},{"key":"key\n\t\t\t\t20170620071201_B44","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1038\/nature04152","article-title":"An induced-fit mechanism to promote peptide bond formation and exclude hydrolysis of peptidyl-tRNA","volume":"438","year":"2005","journal-title":"Nature"},{"key":"key\n\t\t\t\t20170620071201_B45","first-page":"225","article-title":"A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits","volume":"9","year":"2002","journal-title":"Nat. Struct. Biol."}],"container-title":["Nucleic Acids Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/nar\/article-pdf\/45\/11\/6717\/17755914\/gkx195.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,19]],"date-time":"2019-09-19T14:37:04Z","timestamp":1568903824000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/nar\/article-lookup\/doi\/10.1093\/nar\/gkx195"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,21]]},"references-count":45,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2017,3,21]]},"published-print":{"date-parts":[[2017,6,20]]}},"URL":"https:\/\/doi.org\/10.1093\/nar\/gkx195","relation":{},"ISSN":["0305-1048","1362-4962"],"issn-type":[{"value":"0305-1048","type":"print"},{"value":"1362-4962","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,21]]}}}