{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T12:30:44Z","timestamp":1769085044422,"version":"3.49.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2018,8,10]],"date-time":"2018-08-10T00:00:00Z","timestamp":1533859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Technique Innovation Program of Hubei Province","award":["2016ABA124"],"award-info":[{"award-number":["2016ABA124"]}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"crossref","award":["2016PY052"],"award-info":[{"award-number":["2016PY052"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"DOI":"10.1093\/nar\/gky739","type":"journal-article","created":{"date-parts":[[2018,8,3]],"date-time":"2018-08-03T11:11:35Z","timestamp":1533294695000},"source":"Crossref","is-referenced-by-count":11,"title":["Capping-RACE: a simple, accurate, and sensitive 5\u2032 RACE method for use in prokaryotes"],"prefix":"10.1093","author":[{"given":"Fang","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology and Key Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology and Key Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan-Chun","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology and Key Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0888-1987","authenticated-orcid":false,"given":"Zheng-Fei","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology and Key Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2018,8,10]]},"reference":[{"key":"key\n\t\t\t\t20180810034556_B1","first-page":"615","article-title":"Regulatory RNAs in bacteria","volume":"136","author":"Waters","year":"2009","journal-title":"Cell"},{"key":"key\n\t\t\t\t20180810034556_B2","first-page":"8998","article-title":"Rapid production of full-length cdnas from rare transcripts: amplification using a single gene-specific oligonucleotide primer","volume":"85","author":"Frohman","year":"1988","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"key\n\t\t\t\t20180810034556_B3","first-page":"5673","article-title":"One-sided polymerase chain reaction: the amplification of cdna","volume":"86","author":"Ohara","year":"1989","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"key\n\t\t\t\t20180810034556_B4","first-page":"217","article-title":"Polymerase chain reaction with single-sided specificity: analysis of t cell receptor delta chain","volume":"243","author":"Loh","year":"1989","journal-title":"Science"},{"key":"key\n\t\t\t\t20180810034556_B5","first-page":"5227","article-title":"Oligodeoxyribonucleotide ligation to single-stranded cdnas: a new tool for cloning 5\u2032 ends of mrnas and for constructing cdna libraries by in vitro amplification","volume":"19","author":"Edwards","year":"1991","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20180810034556_B6","first-page":"9823","article-title":"Ligation-anchored pcr: a simple amplification technique with single-sided specificity","volume":"89","author":"Troutt","year":"1992","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"key\n\t\t\t\t20180810034556_B7","first-page":"4954","article-title":"Mapping the 5\u2032 and 3\u2032 ends of tetrahymena thermophila mrnas using rna ligase mediated amplification of cdna ends (rlm-race)","volume":"21","author":"Liu","year":"1993","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20180810034556_B8","first-page":"1683","article-title":"A highly sensitive method for mapping the 5\u2032 termini of mrnas","volume":"21","author":"Fromont-Racine","year":"1993","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20180810034556_B9","first-page":"171","article-title":"Oligo-capping: a simple method to replace the cap structure of eukaryotic mrnas with oligoribonucleotides","volume":"138","author":"Maruyama","year":"1994","journal-title":"Gene"},{"key":"key\n\t\t\t\t20180810034556_B10","first-page":"e31","article-title":"Capselect: a highly sensitive method for 5\u2032cap-dependent enrichment of full-length cdna in pcr-mediated analysis of mrnas","volume":"27","author":"Schmidt","year":"1999","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20180810034556_B11","first-page":"E96","article-title":"A simple and reliable 5\u2032-race approach","volume":"28","author":"Schramm","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20180810034556_B12","first-page":"1558","article-title":"Amplification of cdna ends based on template-switching effect and step-out pcr","volume":"27","author":"Matz","year":"1999","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20180810034556_B13","first-page":"187","article-title":"Simultaneous amplification of 5\u2032 and 3\u2032 cDNA ends based on template-switching effect and inverse PCR","volume":"40","author":"Huang","year":"2006","journal-title":"BioTechniques"},{"key":"key\n\t\t\t\t20180810034556_B14","first-page":"e194","article-title":"Targeted rapid amplification of cDNA ends (T-RACE)\u2013an improved RACE reaction through degradation of non-target sequences","volume":"38","author":"Bower","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20180810034556_B15","first-page":"9","article-title":"Prokaryotic transcriptomics: a new view on regulation, physiology and pathogenicity","volume":"11","author":"Sorek","year":"2009","journal-title":"Nat. Rev. Genet."},{"key":"key\n\t\t\t\t20180810034556_B16","first-page":"250","article-title":"The primary transcriptome of the major human pathogen Helicobacter pylori","volume":"464","author":"Sharma","year":"2010","journal-title":"Nature"},{"key":"key\n\t\t\t\t20180810034556_B17","first-page":"97","article-title":"Differential RNA-seq: the approach behind and the biological insight gained","volume":"19","author":"Sharma","year":"2014","journal-title":"Curr. Opin. Microbiol."},{"key":"key\n\t\t\t\t20180810034556_B18","first-page":"6620","article-title":"Experimental discovery of small RNAs in Staphylococcus aureus reveals a riboregulator of central metabolism","volume":"38","author":"Bohn","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20180810034556_B19","first-page":"123","article-title":"The primary transcriptome of barley chloroplasts: numerous noncoding RNAs and the dominating role of the Plastid-Encoded RNA polymerase","volume":"24","author":"Zhelyazkova","year":"2012","journal-title":"Plant Cell"},{"key":"key\n\t\t\t\t20180810034556_B20","first-page":"1121","article-title":"Genome-wide mapping of transcriptional start sites defines an extensive leaderless transcriptome in mycobacterium tuberculosis","volume":"5","author":"Cortes","year":"2013","journal-title":"Cell Rep."},{"key":"key\n\t\t\t\t20180810034556_B21","first-page":"2056","article-title":"Comparative transcriptomics of two environmentally relevant cyanobacteria reveals unexpected transcriptome diversity","volume":"8","author":"Voigt","year":"2014","journal-title":"ISME J."},{"key":"key\n\t\t\t\t20180810034556_B22","first-page":"629","article-title":"Genome-wide identification of transcriptional start sites in the haloarchaeon Haloferax volcanii based on differential RNA-Seq (dRNA-Seq)","volume":"17","author":"Babski","year":"2016","journal-title":"BMC Genomics"},{"key":"key\n\t\t\t\t20180810034556_B23","first-page":"10","article-title":"Genome-Wide transcriptional start site mapping and sRNA identification in the pathogen leptospira interrogans","volume":"7","author":"Zhukova","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"key\n\t\t\t\t20180810034556_B24","first-page":"6147","article-title":"The primary transcriptome of Neisseria meningitidis and its interaction with the RNA chaperone Hfq","volume":"45","author":"Heidrich","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20180810034556_B25","first-page":"e01966-17","article-title":"Differential RNA sequencing implicates sulfide as the master regulator of S0 metabolism in chlorobaculum tepidum and other green sulfur bacteria","volume":"84","author":"Hilzinger","year":"2018","journal-title":"Appl.Environ.Microbiol."},{"key":"key\n\t\t\t\t20180810034556_B26","first-page":"199","article-title":"A novel enrichment strategy reveals unprecedented number of novel transcription start sites at single base resolution in a model prokaryote and the gut microbiome","volume":"17","author":"Ettwiller","year":"2016","journal-title":"BMC Genomics"},{"key":"key\n\t\t\t\t20180810034556_B27","first-page":"13783","article-title":"Global repositioning of transcription start sites in a plant-fermenting bacterium","volume":"7","author":"Boutard","year":"2016","journal-title":"Nat. Commun."},{"key":"key\n\t\t\t\t20180810034556_B28","first-page":"1013","article-title":"MicroRNA expression profile in RAW264.7 cells in response to brucella melitensis infection","volume":"8","author":"Zheng","year":"2012","journal-title":"Int. J. Biol. Sci."},{"key":"key\n\t\t\t\t20180810034556_B29","first-page":"227","article-title":"A guide for in-house design of template-switch-based 5\u2032 rapid amplification of cDNA ends systems","volume":"397","author":"Pinto","year":"2010","journal-title":"Anal. Biochem."},{"key":"key\n\t\t\t\t20180810034556_B30","first-page":"8864","article-title":"Specificity of RppH-dependent RNA degradation in Bacillus subtilis","volume":"110","author":"Hsieh","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"key\n\t\t\t\t20180810034556_B31","first-page":"D133","article-title":"RegulonDB version 9.0: high-level integration of gene regulation, coexpression, motif clustering and beyond","volume":"44","author":"Gama-Castro","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"key\n\t\t\t\t20180810034556_B32","first-page":"427","article-title":"Transcriptome-wide identification of Hfq-associated RNAs in Brucella suis by deep sequencing","volume":"198","author":"Saadeh","year":"2016","journal-title":"J. Bacteriol."}],"container-title":["Nucleic Acids Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/nar\/advance-article-pdf\/doi\/10.1093\/nar\/gky739\/25448106\/gky739.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T22:58:04Z","timestamp":1589929084000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/nar\/advance-article\/doi\/10.1093\/nar\/gky739\/5068902"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,10]]},"references-count":32,"URL":"https:\/\/doi.org\/10.1093\/nar\/gky739","relation":{},"ISSN":["0305-1048","1362-4962"],"issn-type":[{"value":"0305-1048","type":"print"},{"value":"1362-4962","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,10]]}}}