{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T03:00:14Z","timestamp":1790046014425,"version":"4.0.1"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2019,2,11]],"date-time":"2019-02-11T00:00:00Z","timestamp":1549843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16H04750"],"award-info":[{"award-number":["16H04750"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15F15386"],"award-info":[{"award-number":["15F15386"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18H05501"],"award-info":[{"award-number":["18H05501"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJCR1XMX5"],"award-info":[{"award-number":["JPMJCR1XMX5"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,4,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Cockayne syndrome group B (CSB, also known as ERCC6) protein is involved in many DNA repair processes and essential for transcription-coupled repair (TCR). The central region of CSB has the helicase motif, whereas the C-terminal region contains important regulatory elements for repair of UV- and oxidative stress-induced damages and double-strand breaks (DSBs). A previous study suggested that a small part (\u223c30 residues) within this region was responsible for binding to ubiquitin (Ub). Here, we show that the Ub-binding of CSB requires a larger part of CSB, which was previously identified as a winged-helix domain (WHD) and is involved in the recruitment of CSB to DSBs. We also present the crystal structure of CSB WHD in complex with Ub. CSB WHD folds as a single globular domain, defining a class of Ub-binding domains (UBDs) different from 23 UBD classes identified so far. The second \u03b1-helix and C-terminal extremity of CSB WHD interact with Ub. Together with structure-guided mutational analysis, we identified the residues critical for the binding to Ub. CSB mutants defective in the Ub binding reduced repair of UV-induced damage. This study supports the notion that DSB repair and TCR may be associated with the Ub-binding of CSB.<\/jats:p>","DOI":"10.1093\/nar\/gkz081","type":"journal-article","created":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T13:49:25Z","timestamp":1549028965000},"page":"3784-3794","source":"Crossref","is-referenced-by-count":24,"title":["Structural basis of ubiquitin recognition by the winged-helix domain of Cockayne syndrome group B protein"],"prefix":"10.1093","volume":"47","author":[{"given":"Diane T","family":"Takahashi","sequence":"first","affiliation":[{"name":"Institute for Quantitative Biosciences, The University of Tokyo , Tokyo 113-0032, Japan"},{"name":"Synchrotron Radiation Research Organization, The University of Tokyo , Tokyo 113-0032, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Sato","sequence":"additional","affiliation":[{"name":"Institute for Quantitative Biosciences, The University of Tokyo , Tokyo 113-0032, Japan"},{"name":"Synchrotron Radiation Research Organization, The University of Tokyo , Tokyo 113-0032, Japan"},{"name":"Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo , Chiba 277-8562, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Yamagata","sequence":"additional","affiliation":[{"name":"Institute for Quantitative Biosciences, The University of Tokyo , Tokyo 113-0032, Japan"},{"name":"Synchrotron Radiation Research Organization, The University of Tokyo , Tokyo 113-0032, Japan"},{"name":"Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo , Chiba 277-8562, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakurako","family":"Goto-Ito","sequence":"additional","affiliation":[{"name":"Institute for Quantitative Biosciences, The University of Tokyo , Tokyo 113-0032, Japan"},{"name":"Synchrotron Radiation Research Organization, The University of Tokyo , Tokyo 113-0032, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masafumi","family":"Saijo","sequence":"additional","affiliation":[{"name":"Graduate School of Frontier Biosciences, Osaka University , Yamadaoka 1-3, Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1241-1443","authenticated-orcid":false,"given":"Shuya","family":"Fukai","sequence":"additional","affiliation":[{"name":"Institute for Quantitative Biosciences, The University of Tokyo , Tokyo 113-0032, Japan"},{"name":"Synchrotron Radiation Research Organization, The University of Tokyo , Tokyo 113-0032, Japan"},{"name":"Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo , Chiba 277-8562, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,2,11]]},"reference":[{"key":"2022061113432055100_B1","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1038\/nrm2549","article-title":"Transcription-coupled DNA repair: two decades of progress and surprises","volume":"9","author":"Hanawalt","year":"2008","journal-title":"Nat. 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Genet."},{"key":"2022061113432055100_B5","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1002\/humu.21154","article-title":"Mutation update for the CSB\/ERCC6 and CSA\/ERCC8 genes involved in Cockayne syndrome","volume":"31","author":"Laugel","year":"2010","journal-title":"Hum. Mutat."},{"key":"2022061113432055100_B6","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1086\/301686","article-title":"Molecular analysis of mutations in the CSB (ERCC6) gene in patients with Cockayne syndrome","volume":"62","author":"Mallery","year":"1998","journal-title":"Am. J. Hum. Genet."},{"key":"2022061113432055100_B7","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1038\/ng.2229","article-title":"Mutations in UVSSA cause UV-sensitive syndrome and impair RNA polymerase IIo processing in transcription-coupled nucleotide-excision repair","volume":"44","author":"Nakazawa","year":"2012","journal-title":"Nat. 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