{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T04:12:02Z","timestamp":1772079122600,"version":"3.50.1"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T00:00:00Z","timestamp":1574380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008608","name":"Sumitomo Foundation","doi-asserted-by":"publisher","award":["140384"],"award-info":[{"award-number":["140384"]}],"id":[{"id":"10.13039\/100008608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,24]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Highly sensitive protein quantification enables the detection of a small number of protein molecules that serve as markers\/triggers for various biological phenomena, such as cancer. Here, we describe the development of a highly sensitive protein quantification system called HaloTag protein barcoding. The method involves covalent linking of a target protein to a unique molecule counting oligonucleotide at a 1:1 conjugation ratio based on an azido\u2013cycloalkyne click reaction. The sensitivity of the HaloTag-based barcoding was remarkably higher than that of a conventional luciferase assay. The HaloTag system was successfully validated by analyzing a set of protein-protein interactions, with the identification rate of 44% protein interactions between positive reference pairs reported in the literature. Desmoglein 3, the target antigen of pemphigus vulgaris, an IgG-mediated autoimmune blistering disease, was used in a HaloTag protein barcode assay to detect the anti-DSG3 antibody. The dynamic range of the assay was over 104-times wider than that of a conventional enzyme-linked immunosorbent assay (ELISA). The technology was used to detect anti-DSG3 antibody in patient samples with much higher sensitivity compared to conventional ELISA. Our detection system, with its superior sensitivity, enables earlier detection of diseases possibly allowing the initiation of care\/treatment at an early disease stage.<\/jats:p>","DOI":"10.1093\/nar\/gkz1086","type":"journal-article","created":{"date-parts":[[2019,11,18]],"date-time":"2019-11-18T14:18:09Z","timestamp":1574086689000},"page":"e8-e8","source":"Crossref","is-referenced-by-count":20,"title":["HaloTag-based conjugation of proteins to barcoding-oligonucleotides"],"prefix":"10.1093","volume":"48","author":[{"given":"Junshi","family":"Yazaki","sequence":"first","affiliation":[{"name":"Laboratory for Integrative Genomics, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama City\u00a0230-0045, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Kawashima","sequence":"additional","affiliation":[{"name":"Laboratory for Integrative Genomics, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama City\u00a0230-0045, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taisaku","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Laboratory for Prediction of Cell Systems Dynamics, RIKEN Center for Biosystems Dynamics Research (BDR), Osaka\u00a0565\u20130874, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsuo","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Laboratory for Integrative Genomics, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama City\u00a0230-0045, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayu","family":"Okoshi","sequence":"additional","affiliation":[{"name":"Laboratory for Integrative Genomics, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama City\u00a0230-0045, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Laboratory for Integrative Genomics, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama City\u00a0230-0045, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suguru","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Bioscience, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo 101-0062, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isao","family":"Kii","sequence":"additional","affiliation":[{"name":"Common Facilities Unit, Compass to Healthy Life Research Complex Program, RIKEN Cluster for Science, Technology and Innovation Hub, Kobe\u00a0650-0047, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shohei","family":"Egami","sequence":"additional","affiliation":[{"name":"Laboratory for Skin Homeostasis, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama 230-0045, Japan"},{"name":"Department of Dermatology, Keio University School of Medicine, Tokyo\u00a0160\u20138582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayuki","family":"Amagai","sequence":"additional","affiliation":[{"name":"Laboratory for Skin Homeostasis, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama 230-0045, Japan"},{"name":"Department of Dermatology, Keio University School of Medicine, Tokyo\u00a0160\u20138582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takamitsu","family":"Hosoya","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Bioscience, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo 101-0062, Japan"},{"name":"Laboratory for Chemical Biology, RIKEN Center for Biosystems Dynamics Research (BDR), Kobe 650-0047, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuyuki","family":"Shiroguchi","sequence":"additional","affiliation":[{"name":"Laboratory for Prediction of Cell Systems Dynamics, RIKEN Center for Biosystems Dynamics Research (BDR), Osaka\u00a0565\u20130874, Japan"},{"name":"Laboratory for Immunogenetics, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama 230-0045, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Ohara","sequence":"additional","affiliation":[{"name":"Laboratory for Integrative Genomics, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama City\u00a0230-0045, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,11,22]]},"reference":[{"key":"2020011102341909800_B1","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1158\/1055-9965.EPI-12-1236","article-title":"Biomarker discovery for heterogeneous diseases","volume":"22","author":"Wallstrom","year":"2013","journal-title":"Cancer Epidemiol. 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