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Lack of YbeY results in the accumulation of the 17S rRNA precursor. In contrast to a previous report, we show that Sinorhizobium meliloti YbeY exhibits endoribonuclease activity on single-stranded RNA substrate but not on the double-stranded substrate. This study also identifies the previously unknown metal ion involved in YbeY function to be Zn2+ and shows that the activity of YbeY is enhanced when the occupancy of zinc is increased. We have identified a pre-16S rRNA precursor that accumulates in the S. meliloti \u0394ybeY strain. We also show that \u0394ybeY mutant of Brucella abortus, a mammalian pathogen, also accumulates a similar pre-16S rRNA. The pre-16S species is longer in alpha-proteobacteria than in gamma-proteobacteria. We demonstrate that the YbeY from E. coli and S. meliloti can reciprocally complement the rRNA processing defect in a \u0394ybeY mutant of the other organism. These results establish YbeY as a zinc-dependent single-strand specific endoribonuclease that functions in 16S rRNA processing in both alpha- and gamma-proteobacteria.<\/jats:p>","DOI":"10.1093\/nar\/gkz1095","type":"journal-article","created":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T15:10:42Z","timestamp":1574349042000},"page":"332-348","source":"Crossref","is-referenced-by-count":17,"title":["Sinorhizobium meliloti YbeY is a zinc-dependent single-strand specific endoribonuclease that plays an important role in 16S ribosomal RNA processing"],"prefix":"10.1093","volume":"48","author":[{"given":"Vignesh M P","family":"Babu","sequence":"first","affiliation":[{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siva","family":"Sankari","sequence":"additional","affiliation":[{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A","family":"Budnick","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, VA-MD College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, USA"},{"name":"Department of Microbiology and Molecular Genetics, School of Medicine, University of Pittsburgh, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clayton C","family":"Caswell","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, VA-MD College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham C","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,11,28]]},"reference":[{"key":"2020010522375604400_B1","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1128\/MMBR.68.3.518-537.2004","article-title":"Determination of the core of a minimal bacterial gene set","volume":"68","author":"Gil","year":"2004","journal-title":"Microbiol. 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