{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T04:14:02Z","timestamp":1791605642671,"version":"4.3.4"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2019,12,21]],"date-time":"2019-12-21T00:00:00Z","timestamp":1576886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["GA17-06238S"],"award-info":[{"award-number":["GA17-06238S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["090812\/B\/09\/Z"],"award-info":[{"award-number":["090812\/B\/09\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,2,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>One of the key roles of the 12-subunit eukaryotic translation initiation factor 3 (eIF3) is to promote the formation of the 43S and 48S pre-initiation complexes (PICs). However, particular contributions of its individual subunits to these two critical initiation reactions remained obscure. Here, we adapted formaldehyde gradient cross-linking protocol to translation studies and investigated the efficiency of the 43S and 48S PIC assembly in knockdowns of individual subunits of human eIF3 known to produce various partial subcomplexes. We revealed that eIF3d constitutes an important intermolecular bridge between eIF3 and the 40S subunit as its elimination from the eIF3 holocomplex severely compromised the 43S PIC assembly. Similarly, subunits eIF3a, c and e were found to represent an important binding force driving eIF3 binding to the 40S subunit. In addition, we demonstrated that eIF3c, and eIF3k and l subunits alter the efficiency of mRNA recruitment to 43S PICs in an opposite manner. Whereas the eIF3c knockdown reduces it, downregulation of eIF3k or eIF3l increases mRNA recruitment, suggesting that the latter subunits possess a regulatory potential. Altogether this study provides new insights into the role of human eIF3 in the initial assembly steps of the translational machinery.<\/jats:p>","DOI":"10.1093\/nar\/gkz1185","type":"journal-article","created":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T07:09:50Z","timestamp":1575961790000},"page":"1969-1984","source":"Crossref","is-referenced-by-count":45,"title":["Adapted formaldehyde gradient cross-linking protocol implicates human eIF3d and eIF3c, k and l subunits in the 43S and 48S pre-initiation complex assembly, respectively"],"prefix":"10.1093","volume":"48","author":[{"given":"Anna","family":"Herrmannov\u00e1","sequence":"first","affiliation":[{"name":"Laboratory of Regulation of Gene Expression, Institute of Microbiology of the Czech Academy of Sciences, Prague, Videnska 1083, 142 20, the Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terezie","family":"Prilepskaja","sequence":"first","affiliation":[{"name":"Laboratory of Regulation of Gene Expression, Institute of Microbiology of the Czech Academy of Sciences, Prague, Videnska 1083, 142 20, the Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Wagner","sequence":"first","affiliation":[{"name":"Laboratory of Regulation of Gene Expression, Institute of Microbiology of the Czech Academy of Sciences, Prague, Videnska 1083, 142 20, the Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darina","family":"\u0160ikrov\u00e1","sequence":"first","affiliation":[{"name":"Laboratory of Regulation of Gene Expression, Institute of Microbiology of the Czech Academy of Sciences, Prague, Videnska 1083, 142 20, the Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub","family":"Zeman","sequence":"first","affiliation":[{"name":"Laboratory of Regulation of Gene Expression, Institute of Microbiology of the Czech Academy of Sciences, Prague, Videnska 1083, 142 20, the Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krist\u00fdna","family":"Poncov\u00e1","sequence":"first","affiliation":[{"name":"Laboratory of Regulation of Gene Expression, Institute of Microbiology of the Czech Academy of Sciences, Prague, Videnska 1083, 142 20, the Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8123-8667","authenticated-orcid":false,"given":"Leo\u0161 Shivaya","family":"Val\u00e1\u0161ek","sequence":"first","affiliation":[{"name":"Laboratory of Regulation of Gene Expression, Institute of Microbiology of the Czech Academy of Sciences, Prague, Videnska 1083, 142 20, the Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,12,21]]},"reference":[{"key":"2020022408400753600_B1","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1080\/15476286.2017.1391437","article-title":"eIF3: a factor for human health and disease","volume":"15","author":"Gomes-Duarte","year":"2018","journal-title":"RNA Biol."},{"key":"2020022408400753600_B2","doi-asserted-by":"crossref","first-page":"10948","DOI":"10.1093\/nar\/gkx805","article-title":"Embraced by eIF3: structural and functional insights into the roles of eIF3 across the translation cycle","volume":"45","author":"Val\u00e1\u0161ek","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"2020022408400753600_B3","doi-asserted-by":"crossref","first-page":"20160176","DOI":"10.1098\/rstb.2016.0176","article-title":"Human eIF3: from \u2018blobology\u2019 to biological insight","volume":"372","author":"Cate","year":"2017","journal-title":"Philos. Trans. R. Soc. Lond. B. Biol. Sci."},{"key":"2020022408400753600_B4","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1016\/j.str.2013.04.002","article-title":"Architecture of human translation initiation factor 3","volume":"21","author":"Querol-Audi","year":"2013","journal-title":"Structure"},{"key":"2020022408400753600_B5","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1038\/nature14891","article-title":"Structure of mammalian eIF3 in the context of the 43S preinitiation complex","volume":"525","author":"des\u00a0Georges","year":"2015","journal-title":"Nature"},{"key":"2020022408400753600_B6","doi-asserted-by":"crossref","first-page":"27951","DOI":"10.1074\/jbc.M113.483164","article-title":"Spectrin domain of eukaryotic initiation factor 3a is the docking site for formation of the a:b:i:g subcomplex","volume":"288","author":"Dong","year":"2013","journal-title":"J. Biol. 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