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We demonstrate three applications of this methodology; identifying specific molecules of interest from a dataset containing gigabasepairs of genome; identification of a bacterium from such a dataset and, finally, by locating infecting virus molecules in a background of human genomic material. As a result of the dense fluorescent labelling of the DNA, individual barcodes of the order 40 kb pairs in length can be reliably identified. This means DNA can be prepared for imaging using standard handling and purification techniques. The recorded dataset provides stable physical and electronic records of the total genomic content of a sample that can be readily searched for a molecule or region of interest.<\/jats:p>","DOI":"10.1093\/nar\/gkz212","type":"journal-article","created":{"date-parts":[[2019,3,18]],"date-time":"2019-03-18T16:39:51Z","timestamp":1552927191000},"page":"e68-e68","source":"Crossref","is-referenced-by-count":20,"title":["DNA barcodes for rapid, whole genome, single-molecule analyses"],"prefix":"10.1093","volume":"47","author":[{"given":"Nathaniel O","family":"Wand","sequence":"first","affiliation":[{"name":"School of Chemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK"},{"name":"Physical Sciences of Imaging in the Biomedical Sciences Centre for Doctoral Training, University of Birmingham, Edgbaston,\u00a0Birmingham B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darren A","family":"Smith","sequence":"additional","affiliation":[{"name":"School of Chemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew A","family":"Wilkinson","sequence":"additional","affiliation":[{"name":"School of Chemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashleigh E","family":"Rushton","sequence":"additional","affiliation":[{"name":"School of Chemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2148-1758","authenticated-orcid":false,"given":"Stephen J\u00a0W","family":"Busby","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain B","family":"Styles","sequence":"additional","affiliation":[{"name":"School of Computer Science, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5023-8960","authenticated-orcid":false,"given":"Robert K","family":"Neely","sequence":"additional","affiliation":[{"name":"School of Chemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,3,28]]},"reference":[{"key":"2019070906153789200_B1","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1039\/C6LC01439A","article-title":"Optical DNA mapping in nanofluidic devices: principles and applications","volume":"17","author":"M\u00fcller","year":"2017","journal-title":"Lab Chip"},{"key":"2019070906153789200_B2","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1534\/genetics.115.183483","article-title":"Genome-Wide structural variation detection by genome mapping on nanochannel arrays","volume":"202","author":"Mak","year":"2016","journal-title":"Genetics"},{"key":"2019070906153789200_B3","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1038\/nmeth.3454","article-title":"Assembly and diploid architecture of an individual human genome via single-molecule technologies","volume":"12","author":"Pendleton","year":"2015","journal-title":"Nat. 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