{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:31:05Z","timestamp":1791937865887,"version":"4.3.4"},"reference-count":74,"publisher":"Oxford University Press (OUP)","issue":"13","license":[{"start":{"date-parts":[[2019,5,4]],"date-time":"2019-05-04T00:00:00Z","timestamp":1556928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005010","name":"AIRC","doi-asserted-by":"publisher","award":["IG 2017 Id. 20240"],"award-info":[{"award-number":["IG 2017 Id. 20240"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]},{"name":"TRansforming IDEas in Oncological research","award":["17182"],"award-info":[{"award-number":["17182"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,26]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The influenza A virus (IAV) is a continuous health threat to humans as well as animals due to its recurring epidemics and pandemics. The IAV genome is segmented and the eight negative-sense viral RNAs (vRNAs) are transcribed into positive sense complementary RNAs (cRNAs) and viral messenger RNAs (mRNAs) inside infected host cells. A role for the secondary structure of IAV mRNAs has been hypothesized and debated for many years, but knowledge on the structure mRNAs adopt in vivo is currently missing. Here we solve, for the first time, the in vivo secondary structure of IAV mRNAs in living infected cells. We demonstrate that, compared to the in vitro refolded structure, in vivo IAV mRNAs are less structured but exhibit specific locally stable elements. Moreover, we show that the targeted disruption of these high-confidence structured domains results in an extraordinary attenuation of IAV replicative capacity. Collectively, our data provide the first comprehensive map of the in vivo structural landscape of IAV mRNAs, hence providing the means for the development of new RNA-targeted antivirals.<\/jats:p>","DOI":"10.1093\/nar\/gkz318","type":"journal-article","created":{"date-parts":[[2019,4,23]],"date-time":"2019-04-23T19:14:51Z","timestamp":1556046891000},"page":"7003-7017","source":"Crossref","is-referenced-by-count":72,"title":["In vivo analysis of influenza A mRNA secondary structures identifies critical regulatory motifs"],"prefix":"10.1093","volume":"47","author":[{"given":"Lisa Marie","family":"Simon","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze della Vita e Biologia dei Sistemi, Universit\u00e0 di Torino, Via Accademia Albertina 13, 10123 Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edoardo","family":"Morandi","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze della Vita e Biologia dei Sistemi, Universit\u00e0 di Torino, Via Accademia Albertina 13, 10123 Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Luganini","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze della Vita e Biologia dei Sistemi, Universit\u00e0 di Torino, Via Accademia Albertina 13, 10123 Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio","family":"Gribaudo","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze della Vita e Biologia dei Sistemi, Universit\u00e0 di Torino, Via Accademia Albertina 13, 10123 Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Martinez-Sobrido","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas H","family":"Turner","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Center for RNA Biology, University of Rochester, Rochester, NY 14627, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Oliviero","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze della Vita e Biologia dei Sistemi, Universit\u00e0 di Torino, Via Accademia Albertina 13, 10123 Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danny","family":"Incarnato","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze della Vita e Biologia dei Sistemi, Universit\u00e0 di Torino, Via Accademia Albertina 13, 10123 Torino, Italy"},{"name":"Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG, Groningen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,5,4]]},"reference":[{"key":"2019072309505613000_B1","doi-asserted-by":"crossref","first-page":"3960","DOI":"10.1016\/j.vaccine.2018.05.057","article-title":"Economic burden of seasonal influenza in the United States","volume":"36","author":"Putri","year":"2018","journal-title":"Vaccine"},{"key":"2019072309505613000_B2","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3201\/eid1209.05-0979","article-title":"1918 Influenza: the mother of all pandemics","volume":"12","author":"Taubenberger","year":"2006","journal-title":"Emerging Infect. 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