{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:29:46Z","timestamp":1787009386371,"version":"build-2736575974"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T00:00:00Z","timestamp":1557360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["18-02014S"],"award-info":[{"award-number":["18-02014S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Stop codon readthrough\u2014the decoding of a stop codon by a near-cognate tRNA\u2014is employed by viruses to balance levels of enzymatic and structural proteins and by eukaryotic cells to enable isoform-specific protein synthesis in response to external stimuli. Owing to the prevalence of premature termination codons in human disease, readthrough has emerged as an attractive therapeutic target. A growing list of various features, for example the +4 nucleotide immediately following the stop codon, modulate readthrough levels, underscoring the need for systematic investigation of readthrough. Here, we identified and described a complete group of yeast tRNAs that induce readthrough in the stop-codon tetranucleotide manner when overexpressed, designated readthrough-inducing tRNAs (rti-tRNAs). These rti-tRNAs are the keystones of YARIS (yeast applied readthrough inducing system), a reporter-based assay enabling simultaneous detection of readthrough levels at all twelve stop-codon tetranucleotides and as a function of the complete set of rti-tRNAs. We demonstrate the utility of YARIS for systematic study of translation readthrough by employing it to interrogate the effects of natural rti-tRNA modifications, as well as various readthrough-inducing drugs\u00a0(RTIDs). This analysis identified a variety of genetic interactions demonstrating the power of YARIS to characterize existing and identify novel RTIDs.<\/jats:p>","DOI":"10.1093\/nar\/gkz346","type":"journal-article","created":{"date-parts":[[2019,4,25]],"date-time":"2019-04-25T15:20:13Z","timestamp":1556205613000},"page":"6339-6350","source":"Crossref","is-referenced-by-count":20,"title":["Yeast applied readthrough inducing system (YARIS): an invivo assay for the comprehensive study of translational readthrough"],"prefix":"10.1093","volume":"47","author":[{"given":"Petra","family":"Beznoskov\u00e1","sequence":"first","affiliation":[{"name":"Laboratory of Regulation of Gene Expression, Institute of Microbiology ASCR, Videnska 1083, 142 20\u00a0Prague, the Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"Pavl\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"Laboratory of Regulation of Gene Expression, Institute of Microbiology ASCR, Videnska 1083, 142 20\u00a0Prague, the Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub","family":"Zeman","sequence":"additional","affiliation":[{"name":"Laboratory of Regulation of Gene Expression, Institute of Microbiology ASCR, Videnska 1083, 142 20\u00a0Prague, the Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"Echeverr\u00eda\u00a0Aitken","sequence":"additional","affiliation":[{"name":"Biology Department and Biochemistry Program, Vassar College, 124 Raymond Avenue, Poughkeepsie 12601, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leo\u0161 S","family":"Val\u00e1\u0161ek","sequence":"additional","affiliation":[{"name":"Laboratory of Regulation of Gene Expression, Institute of Microbiology ASCR, Videnska 1083, 142 20\u00a0Prague, the Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,5,9]]},"reference":[{"key":"2019070906353891300_B1","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1080\/15476286.2015.1068497","article-title":"Translational readthrough potential of natural termination codons in eucaryotes\u2013The impact of RNA sequence","volume":"12","author":"Dabrowski","year":"2015","journal-title":"RNA Biol."},{"key":"2019070906353891300_B2","doi-asserted-by":"crossref","first-page":"e03640","DOI":"10.7554\/eLife.03640","article-title":"Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals","volume":"3","author":"Schueren","year":"2014","journal-title":"Elife"},{"key":"2019070906353891300_B3","doi-asserted-by":"crossref","first-page":"8928","DOI":"10.1093\/nar\/gku608","article-title":"Evidence of efficient stop codon readthrough in four mammalian genes","volume":"42","author":"Loughran","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"2019070906353891300_B4","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/0005-2787(74)90366-9","article-title":"The readthrough protein A1 is essential for the formation of viable Q beta particles","volume":"374","author":"Hofstetter","year":"1974","journal-title":"Biochim. 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