{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:12:43Z","timestamp":1791231163963,"version":"4.1.0"},"reference-count":99,"publisher":"Oxford University Press (OUP)","issue":"15","license":[{"start":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T00:00:00Z","timestamp":1566864000000},"content-version":"vor","delay-in-days":53,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["417919780"],"award-info":[{"award-number":["417919780"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,9,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Relaxases of the MOBH family are often found on large plasmids, genetic islands and integrative conjugative elements. Many members of this family contain an N-terminal relaxase domain (TraI_2) followed by a disordered middle part and a C-terminal domain of unknown function (TraI_2_C). The TraI_2 domain contains two putative metal-binding motifs, an HD domain motif and an alternative 3H motif. TraI, encoded within the gonococcal genetic island of Neisseria gonorrhoeae, is the prototype of the MOBH family. SAXS experiments showed that TraI_2 and TraI_2_C form globular structures separated by an extended middle domain. The TraI_2 domain cleaves oriT-ssDNA in a site-specific Mn2+ or Co2+ dependent manner. The minimal oriT encompasses 50 nucleotides, requires an inverted repeat 3\u2032 of the nic-site and several nucleotides around nic for efficient cleavage. Surprisingly, no stable covalent relaxase-DNA intermediate was observed. Mutagenesis of conserved tyrosines showed that cleavage was abolished in the Y212A mutant, whereas the Y212F and Y212H mutants retained residual activity. The HD and the alternative 3H motifs were essential for cleavage and the HD domain residues D162 and D267 for metal ion binding. We propose that the active site binds two metal ions, one in a high-affinity and one in a low-affinity site.<\/jats:p>","DOI":"10.1093\/nar\/gkz577","type":"journal-article","created":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T15:12:56Z","timestamp":1561561976000},"page":"8136-8153","source":"Crossref","is-referenced-by-count":10,"title":["DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island"],"prefix":"10.1093","volume":"47","author":[{"given":"Jan-Hendrik","family":"Heilers","sequence":"first","affiliation":[{"name":"Institute for Biology II, Microbiology, Albert Ludwig University Freiburg, 79104\u00a0Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Reiners","sequence":"additional","affiliation":[{"name":"Biochemie I, Heinrich Heine University D\u00fcsseldorf, 40225\u00a0D\u00fcsseldorf, Germany"},{"name":"Center for Structural Studies, Heinrich Heine University, 40225 D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva-Maria","family":"Heller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annika","family":"Golzer","sequence":"additional","affiliation":[{"name":"Institute for Biology II, Microbiology, Albert Ludwig University Freiburg, 79104\u00a0Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sander H J","family":"Smits","sequence":"additional","affiliation":[{"name":"Biochemie I, Heinrich Heine University D\u00fcsseldorf, 40225\u00a0D\u00fcsseldorf, Germany"},{"name":"Center for Structural Studies, Heinrich Heine University, 40225 D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"van\u00a0der\u00a0Does","sequence":"additional","affiliation":[{"name":"Institute for Biology II, Microbiology, Albert Ludwig University Freiburg, 79104\u00a0Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,7,5]]},"reference":[{"key":"2019082704442944200_B1","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1038\/nrg3962","article-title":"Horizontal gene transfer: Building the web of life","volume":"16","author":"Soucy","year":"2015","journal-title":"Nat. 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