{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:46:22Z","timestamp":1790768782401,"version":"4.1.0"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"20","license":[{"start":{"date-parts":[[2019,10,4]],"date-time":"2019-10-04T00:00:00Z","timestamp":1570147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002915","name":"Fondation pour la recherche medicale","doi-asserted-by":"crossref","award":["DCM20181039531"],"award-info":[{"award-number":["DCM20181039531"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007492","name":"Fondation Bettencourt Schueller","doi-asserted-by":"publisher","award":["AGDI-531281"],"award-info":[{"award-number":["AGDI-531281"]}],"id":[{"id":"10.13039\/501100007492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011658","name":"French Infrastructure for Integrated Structural Biology","doi-asserted-by":"publisher","award":["ANR-10-INSB-05-01"],"award-info":[{"award-number":["ANR-10-INSB-05-01"]}],"id":[{"id":"10.13039\/501100011658","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["197785619"],"award-info":[{"award-number":["197785619"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Union's Horizon 2020"},{"name":"Leibniz competition programme","award":["K72\/2017"],"award-info":[{"award-number":["K72\/2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,11,18]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Toscana virus (TOSV) is an arthropod-borne human pathogen responsible for seasonal outbreaks of fever and meningoencephalitis in the Mediterranean basin. TOSV is a segmented negative-strand RNA virus (sNSV) that belongs to the genus phlebovirus (family Phenuiviridae, order Bunyavirales), encompassing other important human pathogens such as Rift Valley fever virus (RVFV). Here, we carried out a structural and functional characterization of the TOSV cap-snatching endonuclease, an N terminal domain of the viral polymerase (L protein) that provides capped 3\u2032OH primers for transcription. We report TOSV endonuclease crystal structures in the apo form, in complex with a di-ketoacid inhibitor (DPBA) and in an intermediate state of inhibitor release, showing details on substrate binding and active site dynamics. The structure reveals substantial folding rearrangements absent in previously reported cap-snatching endonucleases. These include the relocation of the N terminus and the appearance of new structural motifs important for transcription and replication. The enzyme shows high activity rates comparable to other His+ cap-snatching endonucleases. Moreover, the activity is dependent on conserved residues involved in metal ion and substrate binding. Altogether, these results bring new light on the structure and function of cap-snatching endonucleases and pave the way for the development of specific and broad-spectrum antivirals.<\/jats:p>","DOI":"10.1093\/nar\/gkz838","type":"journal-article","created":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T03:33:33Z","timestamp":1569987213000},"page":"10914-10930","source":"Crossref","is-referenced-by-count":28,"title":["Structure and function of the Toscana virus cap-snatching endonuclease"],"prefix":"10.1093","volume":"47","author":[{"given":"Rhian","family":"Jones","sequence":"first","affiliation":[{"name":"Aix-Marseille Universit\u00e9, CNRS, AFMB UMR 7257, 13288 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Lessoued","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, CNRS, AFMB UMR 7257, 13288 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Meier","sequence":"additional","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, Department of Virology, D-20359 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Devignot","sequence":"additional","affiliation":[{"name":"Institute for Virology, FB10-Veterinary Medicine, Justus-Liebig University, D-35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Barata-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, CNRS, AFMB UMR 7257, 13288 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Mate","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, CNRS, AFMB UMR 7257, 13288 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Bragagnolo","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, CNRS, AFMB UMR 7257, 13288 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9737-337X","authenticated-orcid":false,"given":"Friedemann","family":"Weber","sequence":"additional","affiliation":[{"name":"Institute for Virology, FB10-Veterinary Medicine, Justus-Liebig University, D-35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2986-936X","authenticated-orcid":false,"given":"Maria","family":"Rosenthal","sequence":"additional","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, Department of Virology, D-20359 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4977-7948","authenticated-orcid":false,"given":"Juan","family":"Reguera","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, CNRS, AFMB UMR 7257, 13288 Marseille, France"},{"name":"INSERM, AFMB UMR7257,13288 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,10,4]]},"reference":[{"key":"2019111116430996100_B1","doi-asserted-by":"crossref","first-page":"992","DOI":"10.4269\/ajtmh.16-0967","article-title":"The emergence of severe fever with thrombocytopenia syndrome virus","volume":"97","author":"Silvas","year":"2017","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"2019111116430996100_B2","doi-asserted-by":"crossref","first-page":"e0005660","DOI":"10.1371\/journal.pntd.0005660","article-title":"Phlebotomine sand fly-borne pathogens in the mediterranean basin: Human leishmaniasis and phlebovirus infections","volume":"11","author":"Moriconi","year":"2017","journal-title":"PLoS Negl. Trop. Dis."},{"key":"2019111116430996100_B3","doi-asserted-by":"crossref","first-page":"169","DOI":"10.3389\/fpubh.2014.00169","article-title":"Recent outbreaks of rift valley fever in east africa and the middle east","volume":"2","author":"Himeidan","year":"2014","journal-title":"Front. 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Virol."},{"key":"2019111116430996100_B49","doi-asserted-by":"crossref","first-page":"e1005635","DOI":"10.1371\/journal.ppat.1005635","article-title":"Atomic structure and biochemical characterization of an RNA endonuclease in the N Terminus of andes virus L Protein","volume":"12","author":"Fernandez-Garcia","year":"2016","journal-title":"PLOS Pathog."},{"key":"2019111116430996100_B50","doi-asserted-by":"crossref","first-page":"19686","DOI":"10.1074\/jbc.RA118.004373","article-title":"Biochemical and structural studies reveal differences and commonalities among cap-snatching endonucleases from segmented negative-strand RNA viruses","volume":"293","author":"Holm","year":"2018","journal-title":"J. Biol. Chem."},{"key":"2019111116430996100_B51","doi-asserted-by":"crossref","first-page":"2676","DOI":"10.1099\/jgv.0.000941","article-title":"Toscana virus cap-snatching and initiation of transcription","volume":"98","author":"Amroun","year":"2017","journal-title":"J. Gen. Virol."},{"key":"2019111116430996100_B52","doi-asserted-by":"crossref","first-page":"e1007829","DOI":"10.1371\/journal.ppat.1007829","article-title":"Structure of a functional cap-binding domain in Rift Valley fever virus L protein","volume":"15","author":"Gogrefe","year":"2019","journal-title":"PLOS Pathog."}],"container-title":["Nucleic Acids Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/nar\/article-pdf\/47\/20\/10914\/30623869\/gkz838.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/nar\/article-pdf\/47\/20\/10914\/30623869\/gkz838.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,24]],"date-time":"2021-01-24T00:05:56Z","timestamp":1611446756000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/nar\/article\/47\/20\/10914\/5580917"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,4]]},"references-count":52,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2019,10,4]]},"published-print":{"date-parts":[[2019,11,18]]}},"URL":"https:\/\/doi.org\/10.1093\/nar\/gkz838","relation":{},"ISSN":["0305-1048","1362-4962"],"issn-type":[{"value":"0305-1048","type":"print"},{"value":"1362-4962","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,11,18]]},"published":{"date-parts":[[2019,10,4]]}}}