{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T20:30:09Z","timestamp":1792873809458,"version":"4.5.0"},"reference-count":111,"publisher":"Oxford University Press (OUP)","issue":"1","funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000048","name":"American Cancer Society","doi-asserted-by":"publisher","award":["PF-19-157-01-RMC"],"award-info":[{"award-number":["PF-19-157-01-RMC"]}],"id":[{"id":"10.13039\/100000048","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Metabolic reprogramming is a hallmark of cancer that facilitates changes in many adaptive biological processes. Mutations in the tricarboxylic acid cycle enzyme fumarate hydratase (FH) lead to fumarate accumulation and cause hereditary leiomyomatosis and renal cell cancer\u00a0(HLRCC). HLRCC is a rare, inherited disease characterized by the development of non-cancerous smooth muscle tumors of the uterus and skin, and an increased risk of an aggressive form of kidney cancer. Fumarate has been shown to inhibit 2-oxoglutarate-dependent dioxygenases (2OGDDs) involved in the hydroxylation of HIF1\u03b1, as well as in DNA and histone demethylation. However, the link between fumarate accumulation and changes in RNA post-transcriptional modifications has not been defined. Here, we determine the consequences of fumarate accumulation on the activity of different members of the 2OGDD family targeting RNA modifications. By evaluating multiple RNA modifications in patient-derived HLRCC cell lines, we show that mutation of FH selectively affects the levels of N6-methyladenosine (m6A), while the levels of 5-formylcytosine (f5C) in mitochondrial tRNA are unaffected. This supports the hypothesis of a differential impact of fumarate accumulation on distinct RNA demethylases. The observation that metabolites modulate specific subsets of RNA-modifying enzymes offers new insights into the intersection between metabolism and the epitranscriptome.<\/jats:p>","DOI":"10.1093\/narcan\/zcae004","type":"journal-article","created":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T00:46:56Z","timestamp":1705452416000},"source":"Crossref","is-referenced-by-count":16,"title":["Rewiring of RNA methylation by the oncometabolite fumarate in renal cell carcinoma"],"prefix":"10.1093","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6743-2671","authenticated-orcid":false,"given":"Christina M","family":"Fitzsimmons","sequence":"first","affiliation":[{"name":"Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health , Bethesda , MD 20892,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1609-6871","authenticated-orcid":false,"given":"Mariana D","family":"Mandler","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health , Bethesda , MD 20892,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6241-1070","authenticated-orcid":false,"given":"Judith C","family":"Lunger","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health , Bethesda , MD 20892,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9643-8784","authenticated-orcid":false,"given":"Dalen","family":"Chan","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health , Bethesda , MD 20892,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1143-7788","authenticated-orcid":false,"given":"Siddhardha\u00a0S","family":"Maligireddy","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health , Bethesda , MD 20892,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra\u00a0C","family":"Schmiechen","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health , Bethesda , MD 20892,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supuni\u00a0Thalalla","family":"Gamage","sequence":"additional","affiliation":[{"name":"Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute , Frederick , MD\u00a0 21702 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney","family":"Link","sequence":"additional","affiliation":[{"name":"Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute , Frederick , MD\u00a0 21702 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa\u00a0M","family":"Jenkins","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health , Bethesda , MD 20892,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"King","family":"Chan","sequence":"additional","affiliation":[{"name":"Protein Characterization Laboratory, Research Technology Program, Frederick National Laboratory for Cancer Research, National Cancer Institute, National Institutes of Health , Frederick , MD\u00a021701,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thorkell","family":"Andresson","sequence":"additional","affiliation":[{"name":"Protein Characterization Laboratory, Research Technology Program, Frederick National Laboratory for Cancer Research, National Cancer Institute, National Institutes of Health , Frederick , MD\u00a021701,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9198-4089","authenticated-orcid":false,"given":"Daniel\u00a0R","family":"Crooks","sequence":"additional","affiliation":[{"name":"Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health , Bethesda , MD\u00a020892,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0537-7101","authenticated-orcid":false,"given":"Jordan\u00a0L","family":"Meier","sequence":"additional","affiliation":[{"name":"Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute , Frederick , MD\u00a0 21702 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W\u00a0Marston","family":"Linehan","sequence":"additional","affiliation":[{"name":"Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health , Bethesda , MD\u00a020892,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1055-2354","authenticated-orcid":false,"given":"Pedro\u00a0J","family":"Batista","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health , Bethesda , MD 20892,\u00a0 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