{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T22:24:00Z","timestamp":1787955840556,"version":"build-2784847793"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T00:00:00Z","timestamp":1742428800000},"content-version":"vor","delay-in-days":64,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002341","name":"Research Council of Finland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006306","name":"Sigrid Jus\u00e9lius Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006306","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003125","name":"Finnish Cultural Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003125","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005432","name":"North Savo Regional Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005432","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010711","name":"Cancer Foundation Finland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The interplay between the extracellular matrix (ECM) and prostate cancer\u00a0has been shown to increase ECM stiffness, correlating with more aggressive disease forms. However, the impact of ECM stiffness on the androgen receptor (AR), a key target in\u00a0prostate cancer\u00a0treatment, remains elusive. Here, we\u00a0investigated\u00a0whether matrix stiffness influences prostate cancer\u00a0progression, transcriptional regulation, chromatin state, and AR function in AR-positive prostate cancer\u00a0cells under varying ECM stiffness conditions. We utilized ATAC-seq (assay for transposase-accessible chromatin with sequencing) and RNA sequencing under different ECM conditions, along with\u00a0the SUC2 metastatic prostate adenocarcinoma patient dataset, to investigate the role of ECM stiffness in chromatin state and\u00a0androgen response genes, as well as its impact\u00a0on prostate cancer progression. Results demonstrated\u00a0that increased ECM stiffness elevated the expression of genes related to proliferation and differentiation. In contrast, androgen response genes were most highly\u00a0induced in soft ECM conditions. Integrating\u00a0chromatin accessibility with transcriptomic data\u00a0revealed that androgen response genes were more transcriptionally available in soft ECM conditions. Additionally, increased ECM stiffness upregulated genes associated with low overall survival in the SUC2 dataset. Taken together, our results indicate that high expression of hard matrix stiffness genes may\u00a0promote\u00a0prostate cancer progression, leading to more aggressive disease\u00a0forms associated with\u00a0poor survival.<\/jats:p>","DOI":"10.1093\/narcan\/zcaf010","type":"journal-article","created":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T10:39:41Z","timestamp":1742467181000},"source":"Crossref","is-referenced-by-count":11,"title":["Matrix stiffness modulates androgen response genes and chromatin state in prostate cancer"],"prefix":"10.1093","volume":"7","author":[{"given":"Roosa","family":"Kaarij\u00e4rvi","sequence":"first","affiliation":[{"name":"Institute of Biomedicine, University of Eastern Finland , 70210,\u00a0 Kuopio ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi","family":"Kaljunen","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, University of Eastern Finland , 70210,\u00a0 Kuopio 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