{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:15:15Z","timestamp":1790154915746,"version":"4.0.1"},"reference-count":65,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,7,2]],"date-time":"2020-07-02T00:00:00Z","timestamp":1593648000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Approximately one-third of the world\u2019s human population is estimated to have been exposed to the parasite Toxoplasma gondii. Its prevalence is reportedly high in Ethiopia (74.80%) and Zimbabwe (68.58%), and is 40.40% in Nigeria. The adverse effect of this parasite includes a serious congenital disease in the developing fetus of pregnant women. After several efforts to eliminate the disease, only one licensed vaccine \u2018Toxovax\u2019 has been used to avoid congenital infections in sheep. The vaccine has been adjudged expensive coupled with adverse effects and short shelf life. The potential of vaccine to likely revert to virulent strain is a major reason why it has not been found suitable for human use, hence the need for a vaccine that will induce T and B memory cells capable of eliciting longtime immunity against the infection. This study presents immunoinformatics approaches to design a T. gondii-oriented multiepitope subunit vaccine with focus on micronemal proteins for the vaccine construct. The designed vaccine was subjected to antigenicity, immunogenicity, allergenicity and physicochemical parameter analyses. A 657-amino acid multiepitope vaccine was designed with the antigenicity probability of 0.803. The vaccine construct was classified as stable, non-allergenic, and highly immunogenic, thereby indicating the safety of the vaccine construct for human use.<\/jats:p>","DOI":"10.1093\/nargab\/lqaa048","type":"journal-article","created":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T20:24:00Z","timestamp":1593635040000},"source":"Crossref","is-referenced-by-count":24,"title":["Development of multiepitope subunit protein vaccines\u00a0against Toxoplasma gondii using an immunoinformatics approach"],"prefix":"10.1093","volume":"2","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6649-4857","authenticated-orcid":false,"given":"Olugbenga S","family":"Onile","sequence":"first","affiliation":[{"name":"Biotechnology Programme, Department of Biological Sciences, Elizade University, 340211, Ilara-Mokin, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glory J","family":"Ojo","sequence":"additional","affiliation":[{"name":"Biotechnology Programme, Department of Biological Sciences, Elizade University, 340211, Ilara-Mokin, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bolaji Fatai","family":"Oyeyemi","sequence":"additional","affiliation":[{"name":"Molecular Biology Group, Department of Science Technology, The Federal Polytechnic, 360231, Ado-Ekiti, Ekiti State, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gbenga O","family":"Agbowuro","sequence":"additional","affiliation":[{"name":"Biotechnology Programme, Department of Biological Sciences, Elizade University, 340211, Ilara-Mokin, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeyinka I","family":"Fadahunsi","sequence":"additional","affiliation":[{"name":"Biotechnology Programme, Department of Biological Sciences, Elizade University, 340211, Ilara-Mokin, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,7,1]]},"reference":[{"key":"2020103008534351100_B1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.7774\/cevr.2018.7.2.93","article-title":"Recent progress in microneme-based vaccines development against Toxoplasma gondii","volume":"7","author":"Foroutan","year":"2018","journal-title":"Clin. Exp. Vaccine Res."},{"key":"2020103008534351100_B2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3389\/fpubh.2019.00025","article-title":"Mortality patterns of toxoplasmosis and its comorbidities in Tanzania: a 10-year retrospective hospital-based survey","volume":"7","author":"Mboera","year":"2019","journal-title":"Front. Public Health"},{"key":"2020103008534351100_B3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1076\/ocii.10.1.1.10330","article-title":"Toxoplasmosis, an overview with emphasis on ocular involvement","volume":"10","author":"Klaren","year":"2002","journal-title":"Ocul. Immunol. Inflamm."},{"key":"2020103008534351100_B4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1043-4526(10)60001-0","article-title":"Transmission of toxoplasmosis (Toxoplasma gondii) by foods","volume":"60","author":"Pereira","year":"2010","journal-title":"Adv. Food Nutr. Res."},{"key":"2020103008534351100_B5","doi-asserted-by":"crossref","first-page":"194","DOI":"10.14202\/vetworld.2017.194-208","article-title":"Toxoplasma gondii infection in meat animals from Africa: systematic review and meta-analysis of sero-epidemiological studies","volume":"10","author":"Tonouhewa","year":"2017","journal-title":"Vet. World"},{"key":"2020103008534351100_B6","doi-asserted-by":"crossref","first-page":"19600","DOI":"10.2807\/ese.15.25.19600-en","article-title":"Congenital toxoplasmosis in France in 2007: first results from a national surveillance system","volume":"15","author":"Villena","year":"2010","journal-title":"Eurosurveillance"},{"key":"2020103008534351100_B7","doi-asserted-by":"crossref","first-page":"2199","DOI":"10.1016\/j.ophtha.2009.04.042","article-title":"Congenital toxoplasmosis in southeastern Brazil: results of early ophthalmologic examination of a large cohort of neonates","volume":"116","author":"Vasconcelos-Santos","year":"2009","journal-title":"Ophthalmology"},{"key":"2020103008534351100_B8","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.cmi.2020.01.008","article-title":"Global prevalence of latent toxoplasmosis in pregnant women: a systematic review and meta-analysis","volume":"26","author":"Rostami","year":"2020","journal-title":"Clin. Microbiol. Infect."},{"key":"2020103008534351100_B9","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.jogoh.2017.12.003","article-title":"An updated literature review on maternal\u2013fetal and reproductive disorders of Toxoplasma gondii infection","volume":"47","author":"Fallahi","year":"2018","journal-title":"J. Gynecol. Obstet. Hum. Reprod."},{"key":"2020103008534351100_B10","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1007\/s12639-015-0735-4","article-title":"Seroprevalence of toxoplasmosis in diabetic pregnant women in southwestern of Iran","volume":"40","author":"Saki","year":"2016","journal-title":"J. Parasit. Dis."},{"key":"2020103008534351100_B11","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/j.biopha.2016.05.041","article-title":"Human toxoplasmosis: searching for novel chemotherapeutics","volume":"82","author":"Antczak","year":"2016","journal-title":"Biomed. Pharmacother."},{"key":"2020103008534351100_B12","doi-asserted-by":"crossref","first-page":"101413","DOI":"10.1016\/j.cimid.2020.101413","article-title":"Vaccination with a novel multi-epitope ROP8 DNA vaccine against acute Toxoplasma gondii infection induces strong B and T cell responses in mice","volume":"69","author":"Foroutan","year":"2020","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"2020103008534351100_B13","doi-asserted-by":"crossref","first-page":"104079","DOI":"10.1016\/j.micpath.2020.104079","article-title":"Structural predication and antigenic analysis of ROP16 protein utilizing immunoinformatics methods in order to identification of a vaccine against Toxoplasma gondii: an in silico approach","volume":"142","author":"Ghaffari","year":"2020","journal-title":"Microb. Pathog."},{"key":"2020103008534351100_B14","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.pt.2019.01.005","article-title":"Advances in the development of anti-Toxoplasma gondii vaccines: challenges, opportunities, and perspectives","volume":"35","author":"Wang","year":"2019","journal-title":"Trends Parasitol."},{"key":"2020103008534351100_B15","doi-asserted-by":"crossref","first-page":"418","DOI":"10.3389\/fimmu.2019.00418","article-title":"GRA24-based DNA vaccine prolongs survival in mice challenged with a virulent Toxoplasma gondii strain","volume":"10","author":"Zheng","year":"2019","journal-title":"Front. Immunol."},{"key":"2020103008534351100_B16","first-page":"737125","article-title":"Toll-like receptor initiated host defense against Toxoplasma gondii","volume":"2010","author":"Denkers","year":"2010","journal-title":"Biomed. Res. Int."},{"key":"2020103008534351100_B17","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1007\/s00281-012-0339-3","article-title":"Immune response and immunopathology during toxoplasmosis","volume":"34","author":"Dupont","year":"2012","journal-title":"Semin. Immunopathol."},{"key":"2020103008534351100_B18","doi-asserted-by":"crossref","first-page":"3859","DOI":"10.1038\/s41467-018-06228-8","article-title":"A naturally protective epitope of limited variability as an influenza vaccine target","volume":"9","author":"Thompson","year":"2018","journal-title":"Nat. Commun."},{"key":"2020103008534351100_B19","doi-asserted-by":"crossref","first-page":"174","DOI":"10.5455\/javar.2019.f329","article-title":"Epitope-based vaccine as a universal vaccination strategy against Toxoplasma gondii infection: a mini-review","volume":"6","author":"Hajissa","year":"2019","journal-title":"J. Adv. Vet. Anim. Res."},{"key":"2020103008534351100_B20","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1080\/08830180600785827","article-title":"From plasmids to protection: a review of DNA vaccines against infectious diseases","volume":"25","author":"Laddy","year":"2006","journal-title":"Int. Rev. Immunol."},{"key":"2020103008534351100_B21","doi-asserted-by":"crossref","first-page":"e0143087","DOI":"10.1371\/journal.pone.0143087","article-title":"Vaccination with recombinant microneme proteins confers protection against experimental toxoplasmosis in mice","volume":"10","author":"Pinzan","year":"2015","journal-title":"PLoS One"},{"key":"2020103008534351100_B22","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1016\/S0020-7519(01)00257-0","article-title":"Microneme proteins: structural and functional requirements to promote adhesion and invasion by the apicomplexan parasite Toxoplasma gondii","volume":"31","author":"Soldati","year":"2001","journal-title":"Int. J. Parasitol."},{"key":"2020103008534351100_B23","doi-asserted-by":"crossref","first-page":"16720","DOI":"10.1074\/jbc.M111.325928","article-title":"Galactose recognition by the apicomplexan parasite Toxoplasma gondii","volume":"287","author":"Marchant","year":"2012","journal-title":"J. Biol. Chem."},{"key":"2020103008534351100_B24","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1007\/s10096-018-03442-6","article-title":"A systematic review on efficiency of microneme proteins to induce protective immunity against Toxoplasma gondii","volume":"38","author":"Dodangeh","year":"2019","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"2020103008534351100_B25","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.micpath.2019.05.013","article-title":"In silico analysis and expression of a novel chimeric antigen as a vaccine candidate against Toxoplasma gondii","volume":"132","author":"Dodangeh","year":"2019","journal-title":"Microb. Pathog."},{"key":"2020103008534351100_B26","doi-asserted-by":"crossref","first-page":"2936","DOI":"10.1093\/bioinformatics\/btq551","article-title":"High-throughput prediction of protein antigenicity using protein microarray data","volume":"26","author":"Magnan","year":"2010","journal-title":"Bioinformatics"},{"key":"2020103008534351100_B27","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1038\/s41598-018-19456-1","article-title":"Novel immunoinformatics approaches to design multi-epitope subunit vaccine for malaria by investigating Anopheles salivary protein","volume":"8","author":"Pandey","year":"2018","journal-title":"Sci. Rep."},{"key":"2020103008534351100_B28","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1186\/1471-2105-8-424","article-title":"Large-scale validation of methods for cytotoxic T-lymphocyte epitope prediction","volume":"8","author":"Larsen","year":"2007","journal-title":"BMC Bioinformatics"},{"key":"2020103008534351100_B29","doi-asserted-by":"crossref","first-page":"8285","DOI":"10.1038\/s41598-017-08842-w","article-title":"Exploring Leishmania secretory proteins to design B and T cell multi-epitope subunit vaccine using immunoinformatics approach","volume":"7","author":"Khatoon","year":"2017","journal-title":"Sci. Rep."},{"key":"2020103008534351100_B30","doi-asserted-by":"crossref","first-page":"4409","DOI":"10.1038\/s41598-019-40833-x","article-title":"In-silico design of a multi-epitope vaccine candidate against onchocerciasis and related filarial diseases","volume":"9","author":"Shey","year":"2019","journal-title":"Sci. Rep."},{"key":"2020103008534351100_B31","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1745-6150-8-30","article-title":"Designing of interferon-gamma inducing MHC class-II binders","volume":"8","author":"Dhanda","year":"2013","journal-title":"Biol. Direct"},{"key":"2020103008534351100_B32","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1586\/erv.09.160","article-title":"Advances in the design and delivery of peptide subunit vaccines with a focus on Toll-like receptor agonists","volume":"9","author":"Black","year":"2010","journal-title":"Expert Rev. Vaccines"},{"key":"2020103008534351100_B33","doi-asserted-by":"crossref","first-page":"e104351","DOI":"10.1371\/journal.pone.0104351","article-title":"A potential protein adjuvant derived from Mycobacterium tuberculosis Rv0652 enhances dendritic cells-based tumor immunotherapy","volume":"9","author":"Lee","year":"2014","journal-title":"PLoS One"},{"key":"2020103008534351100_B34","doi-asserted-by":"crossref","first-page":"e30839","DOI":"10.1371\/journal.pone.0030839","article-title":"Synthetic Toll like receptor-4 (TLR-4) agonist peptides as a novel class of adjuvants","volume":"7","author":"Shanmugam","year":"2012","journal-title":"PLoS One"},{"key":"2020103008534351100_B35","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1002\/prot.21078","article-title":"Prediction of continuous B-cell epitopes in an antigen using recurrent neural network","volume":"65","author":"Saha","year":"2006","journal-title":"Proteins"},{"key":"2020103008534351100_B36","doi-asserted-by":"crossref","first-page":"e71610","DOI":"10.1371\/journal.pone.0071610","article-title":"Computational and experimental validation of B and T-cell epitopes of the in vivo immune response to a novel malarial antigen","volume":"8","author":"Bergmann-Leitner","year":"2013","journal-title":"PLoS One"},{"key":"2020103008534351100_B37","doi-asserted-by":"crossref","first-page":"2278","DOI":"10.1007\/s00894-014-2278-5","article-title":"AllerTOP v.2: a server for in silico prediction of allergens","volume":"20","author":"Dimitrov","year":"2014","journal-title":"J. Mol. Model."},{"key":"2020103008534351100_B38","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1093\/bioinformatics\/btt619","article-title":"AllergenFP: allergenicity prediction by descriptor fingerprints","volume":"30","author":"Dimitrov","year":"2014","journal-title":"Bioinformatics"},{"key":"2020103008534351100_B39","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1080\/07391102.2015.1135298","article-title":"Febrifugine analogues as Leishmania donovani trypanothione reductase inhibitors: binding energy analysis assisted by molecular docking, ADMET and molecular dynamics simulation","volume":"35","author":"Pandey","year":"2017","journal-title":"J. Biomol. Struct. Dyn."},{"key":"2020103008534351100_B40","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1006\/jmbi.1999.3091","article-title":"Protein secondary structure prediction based on position-specific scoring matrices","volume":"292","author":"Jones","year":"1999","journal-title":"J. Mol. Biol."},{"key":"2020103008534351100_B41","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1038\/nprot.2010.5","article-title":"I-TASSER: a unified platform for automated protein structure and function prediction","volume":"5","author":"Roy","year":"2010","journal-title":"Nat. Protoc."},{"key":"2020103008534351100_B42","doi-asserted-by":"crossref","first-page":"W384","DOI":"10.1093\/nar\/gkt458","article-title":"GalaxyRefine: protein structure refinement driven by side-chain repacking","volume":"41","author":"Heo","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"2020103008534351100_B43","doi-asserted-by":"crossref","first-page":"W407","DOI":"10.1093\/nar\/gkm290","article-title":"ProSA-web: interactive web service for the recognition of errors in three-dimensional structures of proteins","volume":"35","author":"Wiederstein","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"2020103008534351100_B44","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1002\/prot.10286","article-title":"Structure validation by C\u03b1 geometry: \u03d5, \u03c8 and C\u03b2 deviation","volume":"50","author":"Lovell","year":"2003","journal-title":"Proteins Struct. Funct. Bioinform."},{"key":"2020103008534351100_B45","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/nprot.2016.169","article-title":"The ClusPro web server for protein\u2013protein docking","volume":"12","author":"Kozakov","year":"2017","journal-title":"Nat. Protoc."},{"key":"2020103008534351100_B46","doi-asserted-by":"crossref","first-page":"9232","DOI":"10.1038\/s41598-017-09199-w","article-title":"Exploring dengue genome to construct a multi-epitope based subunit vaccine by utilizing immunoinformatics approach to battle against dengue infection","volume":"7","author":"Ali","year":"2017","journal-title":"Sci. Rep."},{"key":"2020103008534351100_B47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.gene.2016.02.047","article-title":"Synonymous codon usage pattern in glycoprotein gene of rabies virus","volume":"584","author":"Morla","year":"2016","journal-title":"Gene"},{"key":"2020103008534351100_B48","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1002\/prot.20264","article-title":"Scoring function for automated assessment of protein structure template quality","volume":"57","author":"Zhang","year":"2004","journal-title":"Proteins"},{"key":"2020103008534351100_B49","doi-asserted-by":"crossref","first-page":"e0003895","DOI":"10.1371\/journal.pntd.0003895","article-title":"Eliminating the neglected tropical diseases: translational science and new technologies","volume":"10","author":"Hotez","year":"2016","journal-title":"PLoS Negl. Trop. Dis."},{"key":"2020103008534351100_B50","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/S0952-7915(03)00083-9","article-title":"Epitope-based vaccines: an update on epitope identification, vaccine design and delivery","volume":"15","author":"Sette","year":"2003","journal-title":"Curr. Opin. Immunol."},{"key":"2020103008534351100_B51","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1186\/s13071-015-1001-4","article-title":"Research advances in microneme protein 3 of Toxoplasma gondii","volume":"8","author":"Wang","year":"2015","journal-title":"Parasit. Vectors"},{"key":"2020103008534351100_B52","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1007\/s00436-010-1742-0","article-title":"Toxoplasma gondii microneme protein 8 (MIC8) is a potential vaccine candidate against toxoplasmosis","volume":"106","author":"Liu","year":"2010","journal-title":"Parasitol. Res."},{"key":"2020103008534351100_B53","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.exppara.2010.05.008","article-title":"Regulation of CD8+ T cell responses to infection with parasitic protozoa","volume":"126","author":"Jordan","year":"2010","journal-title":"Exp. Parasitol."},{"key":"2020103008534351100_B54","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1615\/CritRevImmunol.v32.i2.30","article-title":"Crosstalk between T lymphocytes and dendritic cells","volume":"32","author":"Hivroz","year":"2012","journal-title":"Crit. Rev. Immunol."},{"key":"2020103008534351100_B55","first-page":"1","article-title":"Cell-mediated immunity","volume-title":"xPharm: The Comprehensive Pharmacology Reference","author":"Pross","year":"2008"},{"key":"2020103008534351100_B56","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.jtbi.2014.01.018","article-title":"A novel multi-epitope peptide vaccine against cancer: an in silico approach","volume":"349","author":"Nezafat","year":"2014","journal-title":"J. Theor. Biol."},{"key":"2020103008534351100_B57","doi-asserted-by":"crossref","first-page":"e00053","DOI":"10.1016\/j.fawpar.2019.e00053","article-title":"A one health approach to vaccines against Toxoplasma gondii","volume":"15","author":"Innes","year":"2019","journal-title":"Food Waterborne Parasitol."},{"key":"2020103008534351100_B58","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1093\/intimm\/dxy004","article-title":"Host immune responses to Toxoplasma gondii","volume":"30","author":"Sasai","year":"2018","journal-title":"Int. Immunol."},{"key":"2020103008534351100_B59","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1038\/nri3598","article-title":"Innate immunity to Toxoplasma gondii infection","volume":"14","author":"Yarovinsky","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"2020103008534351100_B60","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.meegid.2017.02.007","article-title":"A novel design of a multi-antigenic, multistage and multi-epitope vaccine against Helicobacter pylori: an in silico approach","volume":"49","author":"Meza","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"2020103008534351100_B61","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1471-2172-7-7","article-title":"CED: a conformational epitope database","volume":"7","author":"Huang","year":"2006","journal-title":"BMC Immunol."},{"key":"2020103008534351100_B62","doi-asserted-by":"crossref","first-page":"259","DOI":"10.2174\/187153007782794371","article-title":"Protein structure based strategies for antigen discovery and vaccine development against malaria and other pathogens","volume":"7","author":"Corradin","year":"2007","journal-title":"Endocr. Metab. Immune Disord. Drug Targets"},{"key":"2020103008534351100_B63","doi-asserted-by":"crossref","first-page":"5997","DOI":"10.4049\/jimmunol.168.12.5997","article-title":"Cutting edge: MyD88 is required for resistance to Toxoplasma gondii infection and regulates parasite-induced IL-12 production by dendritic cells","volume":"168","author":"Scanga","year":"2002","journal-title":"J. Immunol."},{"key":"2020103008534351100_B64","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.chom.2009.06.005","article-title":"Gut commensal bacteria direct a protective immune response against Toxoplasma gondii","volume":"6","author":"Benson","year":"2009","journal-title":"Cell Host Microbe"},{"key":"2020103008534351100_B65","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.4049\/jimmunol.179.2.1129","article-title":"Activation of TLR2 and TLR4 byglycosylphosphatidylinositols derived from Toxoplasma gondii","volume":"179","author":"Debierre-Grockiego","year":"2007","journal-title":"J. Immunol."}],"container-title":["NAR Genomics and Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/nargab\/article-pdf\/2\/3\/lqaa048\/34054709\/lqaa048.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/nargab\/article-pdf\/2\/3\/lqaa048\/34054709\/lqaa048.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T12:59:20Z","timestamp":1604062760000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/nargab\/article\/doi\/10.1093\/nargab\/lqaa048\/5866109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,1]]},"references-count":65,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,9,1]]}},"URL":"https:\/\/doi.org\/10.1093\/nargab\/lqaa048","relation":{},"ISSN":["2631-9268"],"issn-type":[{"value":"2631-9268","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,9]]},"published":{"date-parts":[[2020,7,1]]},"article-number":"lqaa048"}}