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We profiled chromatin accessibility by the assay for transposase-accessible chromatin by sequencing (ATAC-seq) in laboratory-reared A. gambiae mosquitoes experimentally infected with the human malaria parasite Plasmodium falciparum. By integrating ATAC-seq, RNA-seq and ChIP-seq data, we showed a positive correlation between accessibility at promoters and introns, gene expression and active histone marks. By comparing expression and chromatin structure patterns in different tissues, we were able to infer cis-regulatory elements controlling tissue-specific gene expression and to predict the in vivo binding sites of relevant transcription factors. The ATAC-seq assay also allowed the precise mapping of active regulatory regions, including novel transcription start sites and enhancers that were annotated to mosquito immune-related genes. Not only is this study important for advancing our understanding of mechanisms of transcriptional regulation in the mosquito vector of human malaria, but the information we produced also has great potential for developing new mosquito-control and anti-malaria strategies.<\/jats:p>","DOI":"10.1093\/nargab\/lqaa113","type":"journal-article","created":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T08:49:20Z","timestamp":1611218960000},"source":"Crossref","is-referenced-by-count":26,"title":["The regulatory genome of the malaria vector\n                    <i>Anopheles gambiae<\/i>\n                    : integrating chromatin accessibility and gene expression"],"prefix":"10.1093","volume":"3","author":[{"given":"Jos\u00e9 L","family":"Ruiz","sequence":"first","affiliation":[{"name":"Instituto de Parasitolog\u00eda y Biomedicina L\u00f3pez-Neyra (IPBLN), Consejo Superior de Investigaciones Cient\u00edficas, 18016\u00a0Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa C","family":"Ranford-Cartwright","sequence":"additional","affiliation":[{"name":"Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Science, University of Glasgow, Glasgow G12 8QQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4146-9003","authenticated-orcid":false,"given":"Elena","family":"G\u00f3mez-D\u00edaz","sequence":"additional","affiliation":[{"name":"Instituto de Parasitolog\u00eda y Biomedicina L\u00f3pez-Neyra (IPBLN), Consejo Superior de Investigaciones Cient\u00edficas, 18016\u00a0Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,1,20]]},"reference":[{"key":"2021050316543087800_B1","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.cell.2007.01.015","article-title":"The role of chromatin during transcription","volume":"128","author":"Li","year":"2007","journal-title":"Cell"},{"key":"2021050316543087800_B2","doi-asserted-by":"crossref","first-page":"R34","DOI":"10.1186\/gb-2011-12-4-r34","article-title":"The role of chromatin accessibility in directing the widespread, overlapping patterns of Drosophila transcription factor binding","volume":"12","author":"Li","year":"2011","journal-title":"Genome Biol."},{"key":"2021050316543087800_B3","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1038\/nrg3623","article-title":"Dynamic regulation of transcriptional states by chromatin and transcription factors","volume":"15","author":"Voss","year":"2014","journal-title":"Nat. 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