{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T17:45:11Z","timestamp":1784051111067,"version":"3.55.0"},"reference-count":62,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T00:00:00Z","timestamp":1756857600000},"content-version":"vor","delay-in-days":61,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["GNT1112681"],"award-info":[{"award-number":["GNT1112681"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["GNT1129757"],"award-info":[{"award-number":["GNT1129757"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["GNT1185387"],"award-info":[{"award-number":["GNT1185387"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["GNT2011299"],"award-info":[{"award-number":["GNT2011299"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Meiotic crossovers promote correct chromosome segregation and the shuffling of genetic diversity. However, the measurement of crossovers remains challenging, impeding our ability to decipher the molecular mechanisms that are necessary for their formation and regulation. Here we demonstrate a novel repurposing of the single-nucleus Assay for Transposase Accessible Chromatin with sequencing (snATAC-seq) as a simple and high-throughput method to identify and characterize meiotic crossovers from haploid testis nuclei. We first validate the feasibility of obtaining genome-wide coverage from snATAC-seq by using ATAC-seq on bulk haploid mouse testis nuclei, ensuring adequate variant detection for haplotyping. Subsequently, we adapt droplet-based snATAC-seq for crossover detection, revealing &amp;gt;25 000 crossovers in F1 hybrid mice. Comparison between the wild type and a hyper-recombinogenic Fancm-deficient mutant mouse model confirmed an increase in crossover rates in this genotype, however with\u00a0a distribution which was unchanged. We also find that regions with the highest rate of crossover formation are enriched for PRDM9. Our findings demonstrate the utility of snATAC-seq as a robust and scalable tool for high-throughput crossover detection, offering insights into meiotic crossover dynamics and elucidating the underlying molecular mechanisms. It is possible that the research presented here with snATAC-seq of haploid post-meiotic nuclei could be extended into fertility-related diagnostics.<\/jats:p>","DOI":"10.1093\/nargab\/lqaf122","type":"journal-article","created":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T12:08:48Z","timestamp":1754654928000},"source":"Crossref","is-referenced-by-count":1,"title":["A high-resolution meiotic crossover map from single-nucleus ATAC-seq reveals insights into the recombination landscape in mammals"],"prefix":"10.1093","volume":"7","author":[{"given":"Stevan","family":"Novakovic","sequence":"first","affiliation":[{"name":"DNA Repair and Recombination Laboratory, St Vincent's Institute of Medical Research , Fitzroy VIC 3065,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caitlin","family":"Harris","sequence":"additional","affiliation":[{"name":"DNA Repair and Recombination Laboratory, St Vincent's Institute of Medical Research , Fitzroy VIC 3065,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruijie","family":"Liu","sequence":"additional","affiliation":[{"name":"Bioinformatics and Cellular Genomics, St Vincent's Institute of Medical Research , Fitzroy VIC 3065,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davis\u00a0J","family":"McCarthy","sequence":"additional","affiliation":[{"name":"Bioinformatics and Cellular Genomics, St Vincent's Institute of Medical Research , Fitzroy VIC 3065,","place":["Australia"]},{"name":"The Faculty of Medicine, Dentistry and Health Science, The University of Melbourne , Carlton VIC 3010,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0143-8293","authenticated-orcid":false,"given":"Wayne","family":"Crismani","sequence":"additional","affiliation":[{"name":"DNA Repair and Recombination Laboratory, St Vincent's Institute of Medical Research , Fitzroy VIC 3065,","place":["Australia"]},{"name":"The Faculty of Medicine, Dentistry and Health Science, The University of Melbourne , Carlton VIC 3010,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,9,3]]},"reference":[{"key":"2025090401522728900_B1","doi-asserted-by":"crossref","first-page":"a016618","DOI":"10.1101\/cshperspect.a016618","article-title":"Meiotic recombination: the essence of heredity","volume":"7","author":"Hunter","year":"2015","journal-title":"Cold Spring Harb Perspect Biol"},{"key":"2025090401522728900_B2","doi-asserted-by":"publisher","first-page":"a016634","DOI":"10.1101\/cshperspect.a016634","article-title":"Mechanism and regulation of meiotic recombination initiation","volume":"7","author":"Lam","year":"2015","journal-title":"Cold Spring Harb Perspect 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