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Methods that address this are either complicated to scale or customize; long-range PCR is problematic to scale, and probe hybridization panels are expensive to customize. In this study, we developed an RNA-guided CRISPR\u2013Cas9 nuclease-based enrichment strategy combined with long-read sequencing, which achieved up to 60-fold enrichment of the target. Our findings demonstrate that the CRISPR\u2013Cas system is a highly effective method for customizable long-read sequencing of target transcripts, which preserves estimation of relative abundance.<\/jats:p>","DOI":"10.1093\/nargab\/lqag111","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:08:45Z","timestamp":1788520125000},"source":"Crossref","is-referenced-by-count":0,"title":["Customizable host and viral transcript enrichment using CRISPR\u2013Cas9 long-read sequencing for characterization of low-to-moderate abundance isoforms"],"prefix":"10.1093","volume":"8","author":[{"given":"An N T","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity , Victoria 3000 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