{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:42:46Z","timestamp":1772138566140,"version":"3.50.1"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:00:00Z","timestamp":1746662400000},"content-version":"vor","delay-in-days":40,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008931","name":"CEA","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008931","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012681","name":"CNRS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012681","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universit\u00e9 d\u2019Evry-Val d\u2019Essonne"},{"DOI":"10.13039\/501100002915","name":"Fondation pour la recherche medicale","doi-asserted-by":"crossref","award":["ALZ\u00b0201912009904"],"award-info":[{"award-number":["ALZ\u00b0201912009904"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006939","name":"EU","doi-asserted-by":"publisher","award":["HORIZON N\u00b0 101070740"],"award-info":[{"award-number":["HORIZON N\u00b0 101070740"]}],"id":[{"id":"10.13039\/100006939","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006364","name":"Institut National du Cancer","doi-asserted-by":"publisher","award":["N\u00b0 2020-124"],"award-info":[{"award-number":["N\u00b0 2020-124"]}],"id":[{"id":"10.13039\/501100006364","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006364","name":"Institut National du Cancer","doi-asserted-by":"publisher","award":["N\u00b0 2022-078"],"award-info":[{"award-number":["N\u00b0 2022-078"]}],"id":[{"id":"10.13039\/501100006364","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>How cells respond to different signals leading to defined lineages is an open question to understand physiological differentiation leading to the formation of organs and tissues. Among the various morphogens, retinoic acid signaling, via the RXR\/RAR nuclear receptors activation, is a key morphogen of nervous system development and brain homeostasis. Here, we analyze gene expression in \u223c80\u00a0000 cells covering 16 days of monolayer mouse stem cell differentiation driven by the pan-RAR agonist all-trans retinoic acid, the RAR\u03b1 agonist BMS753 or the activation of both RAR\u03b2 and RAR\u03b3 receptors (BMS641\u00a0+\u00a0BMS961). Furthermore, we have elucidated the role of these retinoids for driving nervous tissue formation within 90 days of brain organoid cultures, by analyzing\u00a0&amp;gt;8000 distinct spatial regions over 28 brain organoids. Despite a delayed progression in BMS641\u00a0+\u00a0BMS961, RAR-specific agonists led to a variety of neuronal subtypes, astrocytes, and oligodendrocyte precursors. Spatially resolved transcriptomics performed in organoids revealed spatially distinct RAR isotype expression leading to specialization signatures associated with matured tissues, including a variety of neuronal subtypes, retina-like tissue structure signatures and even the presence of microglia.<\/jats:p>","DOI":"10.1093\/narmme\/ugaf018","type":"journal-article","created":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T23:49:21Z","timestamp":1746661761000},"source":"Crossref","is-referenced-by-count":0,"title":["Decoding transcriptional identity during neuron-astroglia cell fate driven by RAR-specific agonists"],"prefix":"10.1093","volume":"2","author":[{"given":"Ariel","family":"Galindo-Albarr\u00e1n","sequence":"first","affiliation":[{"name":"UMR 8030 G\u00e9nomique M\u00e9tabolique, Genoscope, Institut Fran\u00e7ois Jacob, CEA, CNRS, University of Evry-val-d\u2019Essonne, University Paris-Saclay , 91057 \u00c9vry,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aysis","family":"Koshy","sequence":"additional","affiliation":[{"name":"UMR 8030 G\u00e9nomique M\u00e9tabolique, Genoscope, Institut Fran\u00e7ois Jacob, CEA, CNRS, University of Evry-val-d\u2019Essonne, University Paris-Saclay , 91057 \u00c9vry,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria\u00a0Grazia","family":"Mendoza-Ferri","sequence":"additional","affiliation":[{"name":"UMR 8030 G\u00e9nomique M\u00e9tabolique, Genoscope, Institut Fran\u00e7ois Jacob, CEA, CNRS, University of Evry-val-d\u2019Essonne, University Paris-Saclay , 91057 \u00c9vry,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1809-1727","authenticated-orcid":false,"given":"Marco\u00a0Antonio","family":"Mendoza-Parra","sequence":"additional","affiliation":[{"name":"UMR 8030 G\u00e9nomique M\u00e9tabolique, Genoscope, Institut Fran\u00e7ois Jacob, CEA, CNRS, University of Evry-val-d\u2019Essonne, University Paris-Saclay , 91057 \u00c9vry,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,5,7]]},"reference":[{"key":"2025052713021655900_B1","doi-asserted-by":"publisher","first-page":"2279","DOI":"10.3390\/life13122279","article-title":"Retinoids: mechanisms of action in neuronal cell fate acquisition","volume":"13","author":"Koshy","year":"2023","journal-title":"Life"},{"key":"2025052713021655900_B2","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1021\/cr400161b","article-title":"Retinoic acid actions through mammalian nuclear receptors","volume":"114","author":"Huang","year":"2014","journal-title":"Chem Rev"},{"key":"2025052713021655900_B3","doi-asserted-by":"publisher","first-page":"1816","DOI":"10.3389\/fpsyg.2015.01816","article-title":"Retinoic acid signaling: a new piece in the spoken language puzzle","volume":"6","author":"van\u00a0Rhijn","year":"2015","journal-title":"Front Psychol"},{"key":"2025052713021655900_B4","doi-asserted-by":"publisher","first-page":"538","DOI":"10.1038\/msb.2011.73","article-title":"Dissecting the retinoid-induced differentiation of F9 embryonal stem cells by integrative genomics","volume":"7","author":"Mendoza-Parra","year":"2011","journal-title":"Mol Syst Biol"},{"key":"2025052713021655900_B5","doi-asserted-by":"publisher","first-page":"6197","DOI":"10.1073\/pnas.93.12.6197","article-title":"Cell-type and promoter-context dependent retinoic acid receptor (RAR) redundancies for RAR beta 2 and hoxa-1 activation in F9 and P19 cells can be artefactually generated by gene knockouts","volume":"93","author":"Taneja","year":"1996","journal-title":"Proc Natl Acad Sci USA"},{"key":"2025052713021655900_B6","doi-asserted-by":"publisher","first-page":"1505","DOI":"10.1101\/gr.208926.116","article-title":"Reconstructed cell fate-regulatory programs in stem 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