{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:36:03Z","timestamp":1787312163906,"version":"build-2736575974"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T00:00:00Z","timestamp":1754611200000},"content-version":"vor","delay-in-days":38,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"italian Ministry of Research"},{"name":"European Union\u2014Sixth and Seventh Framework Programmes"},{"name":"EMMA-Infrafrontier","award":["INFRA-CT2008-227490"],"award-info":[{"award-number":["INFRA-CT2008-227490"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The transfer RNA (tRNA) ligase (TRL1) is a highly conserved multidomain protein that is the archetype of the recently characterized Rnl6 clade. This clade distinguishes itself through a distinct C-terminal domain that sets it apart from other RNA ligase families. TRL1 is an essential component of pre-tRNA splicing and the processing of the Ire1p (Inositol-requiring enzyme 1)-dependent noncanonical splicing of the messenger RNA (mRNA) coding for HAC-1 (Homologous to Activating Transcription Factor \/ cAMP Response Element-Binding Protein 1 (ATF\/CREB1), a transcription factor critical for the unfolded protein response (UPR) in the kingdom of fungi. Here, we report the crystal structure of the N-terminal adenylyl transferase domain (LIG) from Candida glabrata (Nakaseomyces glabratus). The asymmetric unit contained two molecules in complex with noncovalently linked adenosine monophosphate (AMP), revealing conformational differences. In comparison to previous studies, we observe two distinct and partially overlapping ligand-binding pockets, implying new specific residues involved in ligand binding and recognition. These insights on TRL1\u2019s ligand adaptability have important implications for the development of targeted therapies.<\/jats:p>","DOI":"10.1093\/narmme\/ugaf028","type":"journal-article","created":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T11:51:09Z","timestamp":1754567469000},"source":"Crossref","is-referenced-by-count":1,"title":["The adaptability of the fungal tRNA ligase\u2019s ATP-binding pocket: a potential target for new antifungal drugs"],"prefix":"10.1093","volume":"2","author":[{"given":"Alastair\u00a0G","family":"McEwen","sequence":"first","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire (IGBMC), Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U964, Centre National de la Recherche Scientifique (CNRS) UMR 7104, Universit\u00e9 de Strasbourg , Illkirch 67404,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Giovinazzo","sequence":"additional","affiliation":[{"name":"Istituto di Biochimica e Biologia Cellulare, Campus Internazionale \u201cA. Buzzati-Traverso,\u201d Dipartimento Scienze Biomediche, Consiglio Nazionale delle Ricerche, via Ramarini 32 , Monterotondo , 00015 Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Poussin-Courmontagne","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire (IGBMC), Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U964, Centre National de la Recherche Scientifique (CNRS) UMR 7104, Universit\u00e9 de Strasbourg , Illkirch 67404,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matilde","family":"Merolle","sequence":"additional","affiliation":[{"name":"Istituto di Biochimica e Biologia Cellulare, Campus Internazionale \u201cA. Buzzati-Traverso,\u201d Dipartimento Scienze Biomediche, Consiglio Nazionale delle Ricerche, via Ramarini 32 , Monterotondo , 00015 Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4454-9543","authenticated-orcid":false,"given":"Glauco\u00a0P","family":"Tocchini-Valentini","sequence":"additional","affiliation":[{"name":"Istituto di Biochimica e Biologia Cellulare, Campus Internazionale \u201cA. Buzzati-Traverso,\u201d Dipartimento Scienze Biomediche, Consiglio Nazionale delle Ricerche, via Ramarini 32 , Monterotondo , 00015 Rome,","place":["Italy"]},{"name":"Dipartimento Scienze Biomediche, European Mouse Mutant Archive (EMMA), INFRAFRONTIER-IMPC, Monterotondo Mouse Clinic, Campus Internazionale \u201cA. Buzzati-Traverso,\u201d Consiglio Nazionale delle Ricerche, via Ramarini 32 , Monterotondo , 00015 Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Pellegrini","sequence":"additional","affiliation":[{"name":"Istituto di Biochimica e Biologia Cellulare, Campus Internazionale \u201cA. Buzzati-Traverso,\u201d Dipartimento Scienze Biomediche, Consiglio Nazionale delle Ricerche, via Ramarini 32 , Monterotondo , 00015 Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe\u00a0D","family":"Tocchini-Valentini","sequence":"additional","affiliation":[{"name":"Istituto di Biochimica e Biologia Cellulare, Campus Internazionale \u201cA. Buzzati-Traverso,\u201d Dipartimento Scienze Biomediche, Consiglio Nazionale delle Ricerche, via Ramarini 32 , Monterotondo , 00015 Rome,","place":["Italy"]},{"name":"Dipartimento Scienze Biomediche, European Mouse Mutant Archive (EMMA), INFRAFRONTIER-IMPC, Monterotondo Mouse Clinic, Campus Internazionale \u201cA. Buzzati-Traverso,\u201d Consiglio Nazionale delle Ricerche, via Ramarini 32 , Monterotondo , 00015 Rome,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,8,8]]},"reference":[{"key":"2025081906563218400_B1","doi-asserted-by":"publisher","first-page":"1117","DOI":"10.1517\/17460441.2013.807245","article-title":"Candidiasis drug discovery and development: new approaches targeting virulence for discovering and identifying new drugs","volume":"8","author":"Pierce","year":"2013","journal-title":"Expert Opin Drug Discovery"},{"key":"2025081906563218400_B2","doi-asserted-by":"publisher","first-page":"4577","DOI":"10.1016\/S0021-9258(18)42872-4","article-title":"Yeast tRNA ligase mutants are nonviable and accumulate tRNA splicing intermediates","volume":"267","author":"Phizicky","year":"1992","journal-title":"J Biol Chem"},{"key":"2025081906563218400_B3","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1016\/S0092-8674(00)81361-6","article-title":"tRNA ligase is required for regulated mRNA splicing in the unfolded protein response","volume":"87","author":"Sidrauski","year":"1996","journal-title":"Cell"},{"key":"2025081906563218400_B4","doi-asserted-by":"publisher","first-page":"2978","DOI":"10.1016\/S0021-9258(17)35882-9","article-title":"Saccharomyces cerevisiae tRNA ligase. Purification of the protein and isolation of the structural gene","volume":"261","author":"Phizicky","year":"1986","journal-title":"J Biol Chem"},{"key":"2025081906563218400_B5","doi-asserted-by":"publisher","first-page":"7445","DOI":"10.1016\/S0021-9258(20)89467-8","article-title":"Deletion analysis of a multifunctional yeast tRNA ligase polypeptide. 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