{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T23:09:04Z","timestamp":1790982544738,"version":"4.1.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T00:00:00Z","timestamp":1599091200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"crossref","award":["SFB TRR 219"],"award-info":[{"award-number":["SFB TRR 219"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Phosphate is absorbed in the small intestine via passive flow and active transport.NaPi-IIb, a type II sodium-dependent phosphate transporter, is considered to mediate active phosphate transport in rodents. To study the regulation of intestinal phosphate transport in chronic kidney disease (CKD), we analyzed the expression levels of NaPi-IIb, pituitary-specific transcription factor 1 (PiT-1) and PiT-2 and the kinetics of intestinal phosphate transport using two CKD models.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>CKD was induced in rats via adenine orThy1 antibody injection. Phosphate uptake by intestinal brush border membrane vesicles (BBMV) and the messenger RNA (mRNA) expression of NaPi-IIb, PiT-1 and PiT-2 were analyzed. The protein expression level of NaPi-IIb was measured by mass spectrometry (e.g. liquid chromatography tandem mass spectrometry).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>In normal rats, phosphate uptake into BBMV consisted of a single saturable component and its Michaelis constant (Km) was comparable to that of NaPi-IIb. The maximum velocity (Vmax) correlated with mRNA and protein levels of NaPi-IIb. In the CKD models, intestinal phosphate uptake consisted of two saturable components. The Vmax of the higher-affinity transport, which is thought to be responsible for NaPi-IIb, significantly decreased and the decrease correlated with reduced NaPi-IIb expression. The Km of the lower-affinity transport was comparable to that of PiT-1 and -2. PiT-1 mRNA expression was much higher than that of PiT-2, suggesting that PiT-1 was mostly responsible for phosphate transport.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>This study suggests that the contribution of NaPi-IIb to intestinal phosphate absorption dramatically decreases in rats with CKD and that a low-affinity alternative to NaPi-IIb, in particular PiT-1, is upregulated in a compensatory manner in CKD.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ndt\/gfaa156","type":"journal-article","created":{"date-parts":[[2020,5,29]],"date-time":"2020-05-29T11:09:09Z","timestamp":1590750549000},"page":"68-75","source":"Crossref","is-referenced-by-count":18,"title":["Evidence of an intestinal phosphate transporter alternative to type IIb sodium-dependent phosphate transporter\u00a0in rats with chronic kidney disease"],"prefix":"10.1093","volume":"36","author":[{"given":"Yasuhiro","family":"Ichida","sequence":"first","affiliation":[{"name":"Research Division, Chugai Pharmaceutical Co., Ltd, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuichi","family":"Ohtomo","sequence":"additional","affiliation":[{"name":"Research Division, Chugai Pharmaceutical Co., Ltd, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tessai","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Translational Research Division, Chugai Pharmaceutical Co., Ltd, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoaki","family":"Murao","sequence":"additional","affiliation":[{"name":"Translational Research Division, Chugai Pharmaceutical Co., Ltd, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Tsuboi","sequence":"additional","affiliation":[{"name":"Research Division, Chugai Pharmaceutical Co., Ltd, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiki","family":"Kawabe","sequence":"additional","affiliation":[{"name":"Research Division, Chugai Pharmaceutical Co., Ltd, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Segawa","sequence":"additional","affiliation":[{"name":"Department of Molecular Nutrition, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0853-5635","authenticated-orcid":false,"given":"Naoshi","family":"Horiba","sequence":"additional","affiliation":[{"name":"Research Division, Chugai Pharmaceutical Co., Ltd, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken-ichi","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Department of Molecular Nutrition, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Floege","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Clinical Immunology, RWTH Aachen University Hospital, Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,9,3]]},"reference":[{"key":"2020122916300230100_gfaa156-B1","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s00424-018-2221-1","article-title":"The role of Slc34a2 in intestinal phosphate absorption and phosphate homeostasis","volume":"471","author":"Marks","year":"2019","journal-title":"Pflugers Arch"},{"key":"2020122916300230100_gfaa156-B2","doi-asserted-by":"crossref","first-page":"14564","DOI":"10.1073\/pnas.95.24.14564","article-title":"Characterization of a murine type II sodium-phosphate cotransporter expressed in mammalian small intestine","volume":"95","author":"Hilfiker","year":"1998","journal-title":"Proc Natl Acad Sci USA"},{"key":"2020122916300230100_gfaa156-B3","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s00424-018-2246-5","article-title":"Clinical aspects of the phosphate transporters NaPi-IIa and NaPi-IIb. 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