{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T17:57:29Z","timestamp":1764784649426,"version":"3.37.3"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T00:00:00Z","timestamp":1619654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"\u2018Programme Hospitalier de Recherche Clinique from the Assistance Publique des H\u00f4pitaux de Paris\u2019","award":["AOR0641"],"award-info":[{"award-number":["AOR0641"]}]},{"name":"Swiss National Science Foundation NCCR Kidney.CH"},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["310030-182312"],"award-info":[{"award-number":["310030-182312"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Amiloride is a competitive blocker of the epithelial sodium (Na) channel in the renal collecting duct. It is a less potent diuretic than thiazides or loop diuretics, but is often used in association with its potassium (K)-sparing profile. Whether amiloride has a hypocalciuric effect similar to thiazides remains unclear. Animal studies and experiments on cell lines suggested that amiloride increases calcium (Ca) reabsorption in the distal nephron, but human studies are scarce.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We performed a post hoc analysis of a study with 48 healthy males (mean \u00b1 standard deviation age, 23.2\u2009\u00b1\u20093.9\u2009years) who were assigned to a high-Na\/low-K diet for 7\u2009days before receiving 20\u2009mg of amiloride orally. Urinary excretions of electrolytes were measured at 3 and 6\u2009h afterwards; we calculated the relative changes in urinary excretion rates after amiloride administration.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The high-Na\/low-K diet led to an expected suppression of plasma renin and aldosterone. Amiloride showed a mild natriuretic effect associated with a decreased kaliuresis. Urinary Ca excretion dropped substantially (by 80%) 3\u2009h after amiloride administration and remained low at the sixth hour. At the same time, fractional excretion of lithium decreased by a third, reflecting an increased proximal tubular reabsorption.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>During a high-Na\/low-K diet, amiloride had a strong acute hypocalciuric effect, most probably mediated by increased proximal Ca reabsorption, even though a distal effect cannot be excluded. Further studies should establish if chronic amiloride or combined amiloride\/thiazide treatment may decrease calciuria more efficiently and be useful in preventing kidney stones.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ndt\/gfab159","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T19:45:07Z","timestamp":1619639107000},"page":"298-303","source":"Crossref","is-referenced-by-count":3,"title":["Acute decrease of urine calcium by amiloride in healthy volunteers under high-sodium diet"],"prefix":"10.1093","volume":"37","author":[{"given":"Dusan","family":"Harmacek","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, University of Lausanne, Lausanne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Blanchard","sequence":"additional","affiliation":[{"name":"INSERM, CIC1418, Paris, France"},{"name":"AP-HP, H\u00f4pital Europ\u00e9en Georges-Pompidou, DMU CARTE, 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France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Azizi","sequence":"additional","affiliation":[{"name":"INSERM, CIC1418, Paris, France"},{"name":"Department of Hypertension, DMU CARTE, AP-HP, H\u00f4pital Europ\u00e9en Georges-Pompidou, Universit\u00e9 de Paris, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4123-4279","authenticated-orcid":false,"given":"Olivier","family":"Bonny","sequence":"additional","affiliation":[{"name":"Department of Medicine, Service of Nephrology, Lausanne University Hospital, Lausanne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,4,29]]},"reference":[{"key":"2022030904264571500_gfab159-B1","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1152\/ajprenal.00437.2003","article-title":"Effect of thiazide on renal gene expression of apical calcium channels and 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