{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T17:17:01Z","timestamp":1783358221253,"version":"3.54.6"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T00:00:00Z","timestamp":1671062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010665","name":"Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["812699"],"award-info":[{"award-number":["812699"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,5,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The water channels aquaporin-1 (AQP1) and AQP7 are abundantly expressed in the peritoneal membrane. While AQP1 facilitates water transport during peritoneal dialysis (PD), the role of AQP7, which mediates glycerol transport during fasting, remains unknown.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We investigated the distribution of AQP7 and AQP1 and used a mouse model of PD to investigate the role of AQP7 in the peritoneal membrane at baseline and after fasting.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Single nucleus RNA-sequencing revealed that AQP7 was mostly detected in mature adipocytes, whereas AQP1 was essentially expressed in endothelial cells. Fasting induced significant decreases in whole body fat, plasma glucose, insulin and triglycerides, as well as higher plasma glycerol and corticosterone levels in mice, paralleled by major decreases in adipocyte size and levels of fatty acid synthase and leptin, and increased levels of hormone-sensitive lipase mRNAs in the peritoneum. Mechanistically, fasting upregulated the expression of AQP1 and AQP7 in the peritoneum, with increased ultrafiltration but no change in small solute transport. Studies based on Aqp1 and Aqp7 knockout mice and RU-486 inhibition demonstrated that the glucocorticoid induction of AQP1 mediates the increase in ultrafiltration whereas AQP7 regulates the size of adipocytes in the peritoneum.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Fasting induces a coordinated regulation of lipolytic and lipogenic factors and aqua(glycero)porins in the peritoneum, driving structural and functional changes. These data yield novel information on the specific roles of aquaporins in the peritoneal membrane and indicate that fasting improves fluid removal in a mouse model of PD.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ndt\/gfac318","type":"journal-article","created":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T15:02:29Z","timestamp":1670943749000},"page":"1408-1420","source":"Crossref","is-referenced-by-count":10,"title":["Fasting influences aquaporin expression, water transport and adipocyte metabolism in the peritoneal membrane"],"prefix":"10.1093","volume":"38","author":[{"given":"Ines P D","family":"Costa","sequence":"first","affiliation":[{"name":"Division of Nephrology, IREC, UCLouvain , Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Hautem","sequence":"additional","affiliation":[{"name":"Division of Nephrology, IREC, UCLouvain , Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3549-7796","authenticated-orcid":false,"given":"Gugliemo","family":"Schiano","sequence":"additional","affiliation":[{"name":"Mechanisms of Inherited Kidney Disorders Group, Institute of Physiology, University of Zurich , Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Uchida","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Tokyo Medical and Dental University , Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoya","family":"Nishino","sequence":"additional","affiliation":[{"name":"Division of Nephrology, IREC, UCLouvain , Brussels, Belgium"},{"name":"Department of Nephrology, Nagasaki University Hospital , Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3744-4767","authenticated-orcid":false,"given":"Olivier","family":"Devuyst","sequence":"additional","affiliation":[{"name":"Division of Nephrology, IREC, UCLouvain , Brussels, Belgium"},{"name":"Mechanisms of Inherited Kidney Disorders Group, Institute of Physiology, University of Zurich , Zurich, Switzerland"},{"name":"Division of Nephrology, Cliniques Universitaires Saint-Luc , Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,12,15]]},"reference":[{"key":"2023061413485212900_bib1","doi-asserted-by":"crossref","first-page":"3238","DOI":"10.1681\/ASN.2016010112","article-title":"The current state of peritoneal dialysis","volume":"27","author":"Mehrotra","year":"2016","journal-title":"J Am Soc Nephrol"},{"key":"2023061413485212900_bib2","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1038\/ki.1991.216","article-title":"Computer simulations of peritoneal fluid transport in CAPD","volume":"40","author":"Rippe","year":"1991","journal-title":"Kidney Int"},{"key":"2023061413485212900_bib3","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1038\/sj.ki.5000285","article-title":"Aquaporin-1 plays an essential role in water permeability and ultrafiltration during peritoneal dialysis","volume":"69","author":"Ni","year":"2006","journal-title":"Kidney Int"},{"key":"2023061413485212900_bib4","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.1056\/NEJMoa2034279","article-title":"AQP1 Promoter variant, water transport, and outcomes in peritoneal dialysis","volume":"385","author":"Morelle","year":"2021","journal-title":"N Engl J Med"},{"key":"2023061413485212900_bib5","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1126\/science.256.5055.385","article-title":"Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein","volume":"256","author":"Preston","year":"1992","journal-title":"Science"},{"key":"2023061413485212900_bib6","first-page":"185","article-title":"Aquaglyceroporins in the kidney: present state of knowledge and prospects","volume":"67","author":"Michalek","year":"2016","journal-title":"J Physiol Pharmacol"},{"key":"2023061413485212900_bib7","doi-asserted-by":"crossref","first-page":"F193","DOI":"10.1152\/ajprenal.00304.2019","article-title":"Aquaporins in the kidney: physiology and pathophysiology","volume":"318","author":"Su","year":"2020","journal-title":"Am J Physiol Renal Physiol"},{"key":"2023061413485212900_bib8","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.tips.2021.10.009","article-title":"Recent breakthroughs and future directions in drugging aquaporins","volume":"43","author":"Salman","year":"2022","journal-title":"Trends Pharmacol Sci"},{"key":"2023061413485212900_bib9","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1146\/annurev.physiol.70.113006.100452","article-title":"A current view of the mammalian aquaglyceroporins","volume":"70","author":"Rojek","year":"2008","journal-title":"Annu Rev Physiol"},{"key":"2023061413485212900_bib10","doi-asserted-by":"crossref","first-page":"F981","DOI":"10.1152\/ajprenal.00098.2014","article-title":"Quantification of osmotic water transport in vivo using fluorescent albumin","volume":"307","author":"Morelle","year":"2014","journal-title":"Am J Physiol Renal Physiol"},{"key":"2023061413485212900_bib11","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1097\/01.ASN.0000053420.37216.9E","article-title":"Corticosteroids induce expression of aquaporin-1 and increase transcellular water transport in rat peritoneum","volume":"14","author":"Stoenoiu","year":"2003","journal-title":"J Am Soc Nephrol"},{"key":"2023061413485212900_bib12","doi-asserted-by":"crossref","first-page":"4142","DOI":"10.1093\/ndt\/gfr533","article-title":"High-dose steroid treatment increases free water transport in peritoneal dialysis patients","volume":"26","author":"de Arteaga","year":"2011","journal-title":"Nephrol Dial Transplant"},{"key":"2023061413485212900_bib13","doi-asserted-by":"crossref","first-page":"17801","DOI":"10.1073\/pnas.0406230101","article-title":"Adaptation to fasting by glycerol transport through aquaporin 7 in adipose tissue","volume":"101","author":"Maeda","year":"2004","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023061413485212900_bib14","doi-asserted-by":"crossref","first-page":"F1195","DOI":"10.1152\/ajprenal.00133.2005","article-title":"Defective water and glycerol transport in the proximal tubules of AQP7 knockout mice [published correction appears in Am J Physiol Renal Physiol. 2006 Sep;291(3):F705]","volume":"289","author":"Sohara","year":"2005","journal-title":"Am J Physiol Renal Physiol"},{"key":"2023061413485212900_bib15","doi-asserted-by":"crossref","first-page":"20782","DOI":"10.1074\/jbc.272.33.20782","article-title":"Cloning and functional expression of a new water channel abundantly expressed in the testis permeable to water, glycerol, and urea","volume":"272","author":"Ishibashi","year":"1997","journal-title":"J Biol Chem"},{"key":"2023061413485212900_bib16","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1006\/bbrc.1997.7769","article-title":"Molecular cloning and expression of a novel human aquaporin from adipose tissue with glycerol permeability","volume":"241","author":"Kuriyama","year":"1997","journal-title":"Biochem Biophys Res Commun"},{"key":"2023061413485212900_bib17","doi-asserted-by":"crossref","first-page":"36251","DOI":"10.1074\/jbc.M106040200","article-title":"Genomic structure and insulin-mediated repression of the aquaporin adipose (AQPap), adipose-specific glycerol channel","volume":"276","author":"Kishida","year":"2001","journal-title":"J Biol Chem"},{"key":"2023061413485212900_bib18","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1046\/j.1432-1033.2002.02821.x","article-title":"Human aquaporin adipose (AQPap) gene. Genomic structure, promoter analysis and functional mutation","volume":"269","author":"Kondo","year":"2002","journal-title":"Eur J Biochem"},{"key":"2023061413485212900_bib19","doi-asserted-by":"crossref","first-page":"E586","DOI":"10.1210\/jc.2010-1408","article-title":"Insulin- and leptin-mediated control of aquaglyceroporins in human adipocytes and hepatocytes is mediated via the PI3K\/Akt\/mTOR signaling cascade","volume":"96","author":"Rodr\u00edguez","year":"2011","journal-title":"J Clin Endocrinol Metab"},{"key":"2023061413485212900_bib20","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1007\/s00018-014-1773-2","article-title":"Aquaglyceroporins: implications in adipose biology and obesity","volume":"72","author":"Madeira","year":"2015","journal-title":"Cell Mol Life Sci"},{"key":"2023061413485212900_bib21","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.str.2019.11.011","article-title":"Structural basis for glycerol efflux and selectivity of Human aquaporin 7","volume":"28","author":"de Mar\u00e9","year":"2020","journal-title":"Structure"},{"key":"2023061413485212900_bib22","doi-asserted-by":"crossref","first-page":"495416","DOI":"10.1155\/2010\/495416","article-title":"Peritoneal adipocytes and their role in inflammation during peritoneal dialysis","volume":"2010","author":"Lai","year":"2010","journal-title":"Mediators Inflamm"},{"key":"2023061413485212900_bib23","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1177\/089686089801800204","article-title":"Increase of intra-abdominal fat in patients treated with continuous ambulatory peritoneal dialysis","volume":"18","author":"Fernstr\u00f6m","year":"1998","journal-title":"Perit Dial Int"},{"key":"2023061413485212900_bib24","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1038\/nature13992","article-title":"A comparative encyclopedia of DNA elements in the mouse genome","volume":"515","author":"Yue","year":"2014","journal-title":"Nature"},{"key":"2023061413485212900_bib25","doi-asserted-by":"crossref","first-page":"1260419","DOI":"10.1126\/science.1260419","article-title":"Proteomics. Tissue-based map of the human proteome","volume":"347","author":"Uhl\u00e9n","year":"2015","journal-title":"Science"},{"key":"2023061413485212900_bib26","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1038\/s41586-022-04518-2","article-title":"A single-cell atlas of human and mouse white adipose tissue","volume":"603","author":"Emont","year":"2022","journal-title":"Nature"},{"key":"2023061413485212900_bib27","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.cmet.2020.12.004","article-title":"Plasticity of epididymal adipose tissue in response to diet-Induced obesity at single-nucleus resolution","volume":"33","author":"S\u00e1rv\u00e1ri","year":"2021","journal-title":"Cell Metab"},{"key":"2023061413485212900_bib28","doi-asserted-by":"crossref","first-page":"3573","DOI":"10.1016\/j.cell.2021.04.048","article-title":"Integrated analysis of multimodal single-cell data","volume":"184","author":"Hao","year":"2021","journal-title":"Cell"},{"key":"2023061413485212900_bib29","doi-asserted-by":"crossref","first-page":"4296","DOI":"10.1074\/jbc.273.8.4296","article-title":"Severely impaired urinary concentrating ability in transgenic mice lacking aquaporin-1 water channels","volume":"273","author":"Ma","year":"1998","journal-title":"J Biol Chem"},{"key":"2023061413485212900_bib30","doi-asserted-by":"crossref","first-page":"628094","DOI":"10.1155\/2013\/628094","article-title":"The role of glucocorticoid receptors in dexamethasone-induced apoptosis of neuroprogenitor cells in the hippocampus of rat pups","volume":"2013","author":"Sze","year":"2013","journal-title":"Mediators Inflamm"},{"key":"2023061413485212900_bib31","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1002\/jcsm.12646","article-title":"Myosteatosis rather than sarcopenia associates with non-alcoholic steatohepatitis in non-alcoholic fatty liver disease preclinical models","volume":"12","author":"Nachit","year":"2021","journal-title":"J Cachexia Sarcopenia Muscle"},{"key":"2023061413485212900_bib32","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1111\/j.1523-1755.2005.00304.x","article-title":"Functional and molecular characterization of a peritoneal dialysis model in the C57BL\/6J mouse","volume":"67","author":"Ni","year":"2005","journal-title":"Kidney Int"},{"key":"2023061413485212900_bib33","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1681\/ASN.V114717","article-title":"Vascular proliferation and enhanced expression of endothelial nitric oxide synthase in human peritoneum exposed to long-term peritoneal dialysis","volume":"11","author":"Combet","year":"2000","journal-title":"J Am Soc Nephrol"},{"key":"2023061413485212900_bib34","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1038\/ki.2013.250","article-title":"Water transport across the peritoneal membrane","volume":"85","author":"Devuyst","year":"2014","journal-title":"Kidney Int"},{"key":"2023061413485212900_bib35","doi-asserted-by":"crossref","first-page":"C1562","DOI":"10.1152\/ajpcell.1997.273.5.C1562","article-title":"Aqp1 expression in erythroleukemia cells: genetic regulation of glucocorticoid and chemical induction","volume":"273","author":"Moon","year":"1997","journal-title":"Am J Physiol"},{"key":"2023061413485212900_bib36","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.3390\/ijms17071101","article-title":"Prolonged starvation causes up-regulation of AQP1 in adipose tissue capillaries of AQP7 knock-out mice","volume":"17","author":"Skowronski","year":"2016","journal-title":"Int J Mol Sci"},{"key":"2023061413485212900_bib37","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1210\/en.2015-1890","article-title":"Insulin knockout mice have extended survival but volatile blood glucose levels on leptin therapy","volume":"157","author":"Neumann","year":"2016","journal-title":"Endocrinology"},{"key":"2023061413485212900_bib38","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1093\/jn\/131.7.2067","article-title":"Differences between humans and mice in efficacy of the body fat lowering effect of conjugated linoleic acid: role of metabolic rate","volume":"131","author":"Terpstra","year":"2001","journal-title":"J Nutr"},{"key":"2023061413485212900_bib39","doi-asserted-by":"crossref","first-page":"R165","DOI":"10.1530\/JME-13-0268","article-title":"Metabolic impact of the glycerol channels AQP7 and AQP9 in adipose tissue and liver","volume":"52","author":"Lebeck","year":"2014","journal-title":"J Mol Endocrinol"},{"key":"2023061413485212900_bib40","doi-asserted-by":"crossref","first-page":"2933","DOI":"10.1093\/bioinformatics\/bti473","article-title":"MatInspector and beyond: promoter analysis based on transcription factor binding sites","volume":"21","author":"Cartharius","year":"2005","journal-title":"Bioinformatics"},{"key":"2023061413485212900_bib41","first-page":"E651","article-title":"Insulin, glucagon, and catecholamines in prevention of hypoglycemia during fasting","volume":"256","author":"Boyle","year":"1989","journal-title":"Am J Physiol"},{"key":"2023061413485212900_bib42","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/s13059-017-1187-1","article-title":"Dietary restriction protects from age-associated DNA methylation and induces epigenetic reprogramming of lipid metabolism","volume":"18","author":"Hahn","year":"2017","journal-title":"Genome Biol"},{"key":"2023061413485212900_bib43","doi-asserted-by":"crossref","first-page":"1559325819876780","DOI":"10.1177\/1559325819876780","article-title":"Genome-wide transcriptome analysis reveals intermittent fasting-induced metabolic rewiring in the liver","volume":"17","author":"Ng","year":"2019","journal-title":"Dose Response"},{"key":"2023061413485212900_bib44","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/s13148-017-0340-8","article-title":"36\u00a0h fasting of young men influences adipose tissue DNA methylation of LEP and ADIPOQ in a birth weight-dependent manner","volume":"9","author":"Hjort","year":"2017","journal-title":"Clin Epigenetics"},{"key":"2023061413485212900_bib45","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1093\/ije\/dyu236","article-title":"Adiposity is associated with DNA methylation profile in adipose tissue","volume":"44","author":"Agha","year":"2015","journal-title":"Int J Epidemiol"},{"key":"2023061413485212900_bib46","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.molmet.2019.04.009","article-title":"Epigenetic regulation of diabetogenic adipose morphology","volume":"25","author":"Kerr","year":"2019","journal-title":"Mol Metab"},{"key":"2023061413485212900_bib47","doi-asserted-by":"crossref","first-page":"1731","DOI":"10.1016\/j.metabol.2016.09.004","article-title":"Perilipin 1 binds to aquaporin 7 in human adipocytes and controls its mobility via protein kinase A mediated phosphorylation","volume":"65","author":"Hansen","year":"2016","journal-title":"Metabolism"},{"key":"2023061413485212900_bib48","doi-asserted-by":"crossref","first-page":"R199","DOI":"10.1530\/JME-13-0277","article-title":"Dissecting adipose tissue lipolysis: molecular regulation and implications for metabolic disease","volume":"52","author":"Nielsen","year":"2014","journal-title":"J Mol Endocrinol"},{"key":"2023061413485212900_bib49","doi-asserted-by":"crossref","first-page":"268","DOI":"10.3747\/pdi.2018.00164","article-title":"Effects of excessive body fat accumulation on long-term outcomes during peritoneal dialysis","volume":"39","author":"Kim","year":"2019","journal-title":"Perit Dial Int"}],"container-title":["Nephrology Dialysis Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ndt\/advance-article-pdf\/doi\/10.1093\/ndt\/gfac318\/48845783\/gfac318.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ndt\/article-pdf\/38\/6\/1408\/50603157\/gfac318.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ndt\/article-pdf\/38\/6\/1408\/50603157\/gfac318.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T13:49:53Z","timestamp":1686750593000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ndt\/article\/38\/6\/1408\/6909077"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,15]]},"references-count":49,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,12,15]]},"published-print":{"date-parts":[[2023,5,31]]}},"URL":"https:\/\/doi.org\/10.1093\/ndt\/gfac318","relation":{},"ISSN":["0931-0509","1460-2385"],"issn-type":[{"value":"0931-0509","type":"print"},{"value":"1460-2385","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,6]]},"published":{"date-parts":[[2022,12,15]]}}}