{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:21:40Z","timestamp":1791915700509,"version":"4.3.4"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T00:00:00Z","timestamp":1728345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U23A20410"],"award-info":[{"award-number":["U23A20410"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014125","name":"Fujian Provincial Health Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014125","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Joint Funds for the Innovation of Science and Technology of Fujian province","award":["2021Y9100"],"award-info":[{"award-number":["2021Y9100"]}]},{"name":"Joint Funds for the Innovation of Science and Technology of Fujian province","award":["2023Y9073"],"award-info":[{"award-number":["2023Y9073"]}]},{"name":"Hospital of Fujian Medical University","award":["YJCQN-A-XYF2021"],"award-info":[{"award-number":["YJCQN-A-XYF2021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,4,28]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Oxalate nephropathy is characterized by calcium oxalate crystals deposition, which triggers necrosis of renal tubular epithelial cells and initiates an inflammatory cascade characterized by neutrophil and macrophage activation within the renal microenvironment. Despite the close association of immune cells with acute oxalate nephropathy, the underlying mechanisms still remain unclear. Nerve injury-induced protein 1 (NINJ1) plays an essential role in the induction of plasma membrane rupture (PMR), leading to damage-associated molecular patterns (DAMPs) release and triggering inflammation. We hypothesize that NINJ1-mediated high mobility group box 1 (HMGB1) release from macrophage PMR and neutrophil extracellular traps (NETs) formation synergistically contribute to the progression of acute oxalate nephropathy.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Using a murine model of acute oxalate nephropathy, myeloid cell-specific deletion of Ninj1 mice (Ninj1fl\/flvavcre) and their wild-type littermate control mice (Ninj1wt\/wtvavcre) were administered intraperitoneal injection of 100\u00a0mg\/kg sodium oxalate followed by drinking water with 3% sodium oxalate. Evaluation was conducted on tubular injury and inflammatory cell infiltration. In vitro studies involved isolation and culture of renal proximal tubular epithelial cells, bone marrow\u2013derived macrophages and neutrophils to investigate NETs formation and HMGB1 release.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Targeted deletion of Ninj1 in myeloid cells significantly mitigated oxalate-induced acute kidney injury by suppressing both HMGB1 release and NETs formation in vivo. In vitro investigations demonstrated that HMGB1 release from macrophage PMR and NETs formation in neutrophils mediated by NINJ1 oligomerization, which consequently coordinated to enhance renal tubular epithelial cell death.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our findings elucidate the pivotal role of NINJ1-dependent macrophage PMR and NETs formation in the progression of acute oxalate nephropathy, providing novel insights for its prevention and therapeutic targets.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ndt\/gfae226","type":"journal-article","created":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T03:54:30Z","timestamp":1728446070000},"page":"943-955","source":"Crossref","is-referenced-by-count":20,"title":["NINJ1-mediated macrophage plasma membrane rupture and neutrophil extracellular trap formation contribute to oxalate nephropathy"],"prefix":"10.1093","volume":"40","author":[{"given":"Yujiao","family":"Lin","sequence":"first","affiliation":[{"name":"Blood Purification Research Center, First Affiliated Hospital, Fujian Medical University Department of Nephrology, , Fuzhou ,","place":["China"]},{"name":"Research Center for Metabolic Chronic Kidney Disease, First Affiliated Hospital, Fujian Medical University , Fuzhou ,","place":["China"]},{"name":"National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University Department of Nephrology, , Fuzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yuan","sequence":"additional","affiliation":[{"name":"Blood Purification Research Center, First Affiliated Hospital, Fujian Medical University Department of Nephrology, , Fuzhou ,","place":["China"]},{"name":"Research Center for Metabolic Chronic Kidney Disease, First Affiliated Hospital, Fujian Medical University , Fuzhou ,","place":["China"]},{"name":"National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University Department of Nephrology, , Fuzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keng","family":"Ye","sequence":"additional","affiliation":[{"name":"Blood Purification Research Center, First Affiliated Hospital, Fujian Medical University Department of Nephrology, , Fuzhou ,","place":["China"]},{"name":"Research Center for Metabolic Chronic Kidney Disease, First Affiliated Hospital, Fujian Medical University , Fuzhou ,","place":["China"]},{"name":"National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University Department of Nephrology, , Fuzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhimin","family":"Chen","sequence":"additional","affiliation":[{"name":"Blood Purification Research Center, First Affiliated Hospital, Fujian Medical University Department of Nephrology, , Fuzhou ,","place":["China"]},{"name":"Research Center for Metabolic Chronic Kidney Disease, First Affiliated Hospital, Fujian Medical University , Fuzhou ,","place":["China"]},{"name":"National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University Department of Nephrology, , Fuzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujia","family":"Wang","sequence":"additional","affiliation":[{"name":"Blood Purification Research Center, First Affiliated Hospital, Fujian Medical University Department of Nephrology, , Fuzhou ,","place":["China"]},{"name":"Research Center for Metabolic Chronic Kidney Disease, First Affiliated Hospital, Fujian Medical University , Fuzhou ,","place":["China"]},{"name":"National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University Department of Nephrology, , Fuzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoping","family":"Li","sequence":"additional","affiliation":[{"name":"First Affiliated Hospital, Fujian Medical University Department of Pathology, , Fuzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yankun","family":"Song","sequence":"additional","affiliation":[{"name":"First Affiliated Hospital, Fujian Medical University Department of Pathology, , Fuzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Chen","sequence":"additional","affiliation":[{"name":"First Affiliated Hospital, Fujian Medical University Department of Pathology, , Fuzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huabin","family":"Ma","sequence":"additional","affiliation":[{"name":"Research Center for Metabolic Chronic Kidney Disease, First Affiliated Hospital, Fujian Medical University , Fuzhou ,","place":["China"]},{"name":"Central Laboratory, First Affiliated Hospital, Fujian Medical University , Fuzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6207-545X","authenticated-orcid":false,"given":"Yanfang","family":"Xu","sequence":"additional","affiliation":[{"name":"Blood Purification Research Center, First Affiliated Hospital, Fujian Medical University Department of Nephrology, , Fuzhou ,","place":["China"]},{"name":"Research Center for Metabolic Chronic Kidney Disease, First Affiliated Hospital, Fujian Medical University , Fuzhou ,","place":["China"]},{"name":"National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University Department of Nephrology, , Fuzhou ,","place":["China"]},{"name":"Central Laboratory, First Affiliated Hospital, Fujian Medical University , Fuzhou 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