{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:09:35Z","timestamp":1789754975454,"version":"4.0.1"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,1,10]],"date-time":"2020-01-10T00:00:00Z","timestamp":1578614400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Swiss Kidney Foundation and the Alfred and Erika B\u00e4r-Spycher Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,2,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Improved understanding and assessment of the complex physiology of volume regulation in haemodialysis (HD) patients are required to improve patient care and reduce mortality associated with fluid overload (FO).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We searched for FO-related biomarkers among 184 peptides associated with cardiovascular disease in a cohort of 30 HD patients. First, we assessed the direct impact of HD on the peptides of interest by comparing plasma concentrations before and after treatment. Then, we compared cardiovascular peptide profiles between patients with and without FO as defined by bioimpedance analysis (BIA). The plasma concentration of selected candidate biomarkers for FO was determined by enzyme-linked immunosorbent assay (ELISA) and correlated with previously described FO-related clinical and laboratory parameters. For validation, results were confirmed in an independent cohort of 144 HD patients.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We found seven peptides positively [NT-proBNP, B-type natriuretic peptide (BNP), vascular endothelial growth factor D (VEGFD), tumour necrosis factor-related apoptosis-inducing ligand receptor 2, growth differentiation factor 15, tumour necrosis factor ligand superfamily member 13B, chitinase-3-like protein 1] and five negatively (leptin, renin, epidermal growth factor receptor, interleukin-1 receptor antagonist, myeloblastin) correlated to FO. In addition to natriuretic peptides, VEGFD emerged as third peptide highly correlated with BIA (\u03c1\u2009=\u20090.619, P\u2009&amp;lt;\u20090.0001). In line with this, VEGFD concentration verified by ELISA correlated with BIA, BNP and soluble CD146 but not with vascular endothelial growth factor C (VEGFC). Notably, levels of VEGFD were unrelated to cardiac systolic function (P\u2009=\u20090.63), contrary to BNP (P\u2009=\u20090.0003). Finally, we observed that 1-year all-cause mortality was higher in patients with high BNP (P\u2009=\u20090.0002), FO (defined by BIA, P\u2009=\u20090.04) and high VEGFD (P\u2009=\u20090.02), but not with high VEGFC (P\u2009=\u20090.48).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>VEGFD is a novel FO-related biomarker with unique diagnostic and prognostic properties.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ndt\/gfz281","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T21:06:11Z","timestamp":1575320771000},"page":"529-536","source":"Crossref","is-referenced-by-count":8,"title":["Vascular endothelial growth factor D is a biomarker of fluid overload in haemodialysis patients"],"prefix":"10.1093","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1050-2800","authenticated-orcid":false,"given":"Seraina","family":"von Moos","sequence":"first","affiliation":[{"name":"Department of Nephrology, University Hospital Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Segerer","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Kantonsspital Aarau, Aarau, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Davenport","sequence":"additional","affiliation":[{"name":"UCL Centre for Nephrology, Royal Free Hospital, University College London Medical School, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malha","family":"Sadoune","sequence":"additional","affiliation":[{"name":"INSERM UMR-S 942, MASCOT, Universit\u00e9 de Paris, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerem","family":"Gerritsen","sequence":"additional","affiliation":[{"name":"Department of Nephrology, University Hospital Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Pottecher","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology and Intensive Care, H\u00f4pitaux Universitaires de Strasbourg, H\u00f4pital de Hautepierre, EA3072, F\u00e9d\u00e9ration de M\u00e9decine Translationnelle de Strasbourg (FMTS), Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Ruschitzka","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University Hospital Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Mebazaa","sequence":"additional","affiliation":[{"name":"INSERM UMR-S 942, MASCOT, Universit\u00e9 de Paris, Paris, France"},{"name":"Department of Anesthesiology and Critical Care Medicine, St Louis and Lariboisi\u00e8re University Hospitals, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4028-2869","authenticated-orcid":false,"given":"Mattia","family":"Arrigo","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University Hospital Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro E","family":"Cipp\u00e0","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Regional Hospital of Lugano, Lugano, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,1,10]]},"reference":[{"key":"2021022209000515600_gfz281-B1","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S0140-6736(16)30389-0","article-title":"Controversies and problems of volume control and hypertension in haemodialysis","volume":"388","author":"Ok","year":"2016","journal-title":"Lancet"},{"key":"2021022209000515600_gfz281-B2","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/S0140-6736(16)30508-6","article-title":"The heart and vascular system in dialysis","volume":"388","author":"Wanner","year":"2016","journal-title":"Lancet"},{"key":"2021022209000515600_gfz281-B3","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.1093\/ndt\/gfn707","article-title":"The mortality risk of overhydration in haemodialysis patients","volume":"24","author":"Wizemann","year":"2009","journal-title":"Nephrol Dial Transplant"},{"key":"2021022209000515600_gfz281-B4","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.2215\/CJN.01760210","article-title":"Dry-weight: a concept revisited in an effort to avoid medication-directed approaches for blood pressure control in hemodialysis patients","volume":"5","author":"Agarwal","year":"2010","journal-title":"Clin J Am Soc Nephrol"},{"key":"2021022209000515600_gfz281-B5","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1111\/j.1525-139X.2009.00641.x","article-title":"Can chronic volume overload be recognized and prevented in hemodialysis patients? 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