{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T02:12:52Z","timestamp":1793758372006,"version":"4.6.1"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NEUROSURGERY","NEUROS"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>BACKGROUND:<\/jats:bold>\n                    Axon degeneration leads to cytoskeletal disassembly, metabolism imbalance, and mitochondrial dysfunction during neurodegeneration or nerve injury.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>OBJECTIVE:<\/jats:bold>\n                    In this study, we assess the possibility of mitigating axon degeneration by local injection of mitochondria in a crushed sciatic nerve.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>METHODS:<\/jats:bold>\n                    Sciatic nerve explants cocultured with mitochondria were assessed for the optimal dosage in local injection and nerve regeneration potential. The left sciatic nerve was crushed in Sprague-Dawley rats and then local injection of mitochondria into the distal end of the injured nerve was conducted for further assessment.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>RESULTS:<\/jats:bold>\n                    Mitochondrial coculture attenuated cytoskeletal loss and oxidative stress in isolated nerve explants. In Vivo analyses also showed that mitochondrial transplantation improved animal neurobehaviors, electrophysiology of nerve conduction, and muscle activities. Mitochondria injection significantly attenuated the oxidative stress and increased the expression of neurotrophic factors both in injured nerves and denervated muscles, as well as restored muscular integrity, and increased the pool of muscular progenitor cells and total muscle weight.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>CONCLUSION:<\/jats:bold>\n                    Mitochondria injection can protect injured nerves from axonal degeneration both in Vitro and in Vivo. This improvement was accompanied with the expression of neurotrophic factors as well as the reduction of oxidative stress, which may account for the functional recovery of both injured nerves and denervated muscles.\n                  <\/jats:p>","DOI":"10.1093\/neuros\/nyw090","type":"journal-article","created":{"date-parts":[[2018,2,6]],"date-time":"2018-02-06T11:17:03Z","timestamp":1517915823000},"page":"475-488","source":"Crossref","is-referenced-by-count":51,"title":["Prevention of Axonal Degeneration by Perineurium Injection of Mitochondria in a Sciatic Nerve Crush Injury Model"],"prefix":"10.1227","volume":"80","author":[{"given":"Chi-Chung","family":"Kuo","sequence":"first","affiliation":[{"name":"Department of Life Sciences"},{"name":"Department of Neurology, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung, Taiwan"},{"name":"School of Medicine, Tzu Chi University, Hualien, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Lin","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Life Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu-Lin","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Life Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chien-Yi","family":"Chiang","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Agriculture Biotechnology Center, National Chung-Hsing University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meei-Ling","family":"Sheu","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Agriculture Biotechnology Center, National Chung-Hsing University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu-Chou","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Medical Research"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Jung","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Research"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Sheehan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, University of Virginia, Charlottesville, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hung-Chuan","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Taichung Veterans General Hospital, Taichung, Taiwan"},{"name":"Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2017,3]]},"reference":[{"issue":"(1)","key":"bib1-20231011","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1083\/jcb.201108111","article-title":"Axon degeneration: molecular mechanisms of a self-destruction pathway","volume":"196","author":"Wang","year":"2012","journal-title":"J Cell Biol"},{"issue":"(1)","key":"bib2-20231011","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0143-4160(88)90036-X","article-title":"Proteolysis of tubulin and microtubule-associated proteins 1 and 2 by calpain I and II. 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