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However, achieving high photoresponsivity in tBLG-based sensors typically requires intense illumination. We present an ultrasensitive optoelectronic biosensor integrating tBLG superlattices with Au nanodisks and clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a via DNA origami. By aligning the 9.4\u00b0 tBLG's VHS absorption spectrum with Au nanodisks\u2019 plasmonic resonance at 60\u00a0\u03bcW, we achieve a 7-fold photocurrent enhancement over pristine tBLG. CRISPR-Cas12a-mediated trans-cleavage dynamically modulates the local dielectric environment, enabling sub-femtomolar (44.63\u00a0attomolar, aM) nucleic acid detection without external amplification. Clinical validation using lung cancer samples shows high concordance with quantitative polymerase chain reaction (qPCR), demonstrating real-time, label-free detection of microRNA (miRNA). This hybrid platform combines moir\u00e9-engineered optoelectronics with programmable bio-nanoarrays, offering a scalable solution for precision diagnostics with ultralow detection limits and rapid response times.<\/jats:p>","DOI":"10.1093\/nsr\/nwaf357","type":"journal-article","created":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T11:35:30Z","timestamp":1755948930000},"source":"Crossref","is-referenced-by-count":151,"title":["Ultrasensitive optoelectronic biosensor arrays based on twisted bilayer graphene superlattice"],"prefix":"10.1093","volume":"12","author":[{"given":"Bowen","family":"Du","sequence":"first","affiliation":[{"name":"College of Physics and Optoelectronic Engineering, Interdisciplinary Ctr High Magnet Field Phys, Shenzhen University , Shenzhen 518060"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xilin","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Physics and Optoelectronic Engineering, Interdisciplinary Ctr High Magnet Field Phys, Shenzhen University , Shenzhen 518060"},{"name":"Intensive Care Unit, Shenzhen Key Laboratory of Microbiology in Genomic Modification & Editing and Application, Shenzhen Institute of Translational Medicine, Shenzhen University Medical School, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University Department of Biomedical Engineering, , Shenzhen 518000"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Physics and Optoelectronic Engineering, Interdisciplinary Ctr High Magnet Field Phys, Shenzhen University , Shenzhen 518060"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanqi","family":"Ge","sequence":"additional","affiliation":[{"name":"College of Physics and Optoelectronic Engineering, Interdisciplinary Ctr High Magnet Field Phys, Shenzhen University , Shenzhen 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Medicine, Shenzhen University Medical School, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University Department of Biomedical Engineering, , Shenzhen 518000"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Physics and Optoelectronic Engineering, Interdisciplinary Ctr High Magnet Field Phys, Shenzhen University , Shenzhen 518060"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,8,23]]},"reference":[{"key":"2025100306181524400_bib1","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1038\/s41586-020-2459-6","article-title":"Untying the insulating and superconducting orders in magic-angle 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